Effect of different doses of 2aEuroaminoethoxydiphenyl borate on intestinal ischemia-reperfusion injury

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dc.contributor.authorBasbug, Murat
dc.contributor.authorYildar, Murat
dc.contributor.authorYaman, Ismail
dc.contributor.authorCavdar, Faruk
dc.contributor.authorOzkan, Omer Faruk
dc.contributor.authorAksit, Hasan
dc.contributor.authorOzyigit, Musa Ozgur
dc.date.accessioned2025-01-27T20:44:19Z
dc.date.available2025-01-27T20:44:19Z
dc.date.issued2017
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBackground Acute mesenteric ischemia is a life-threatening clinical entity. 2-Aminoethoxydiphenyl borate (2-APB) is a membrane-permeable modulator of intracellular inositol triphosphate-induced calcium release. We investigated the effects of different 2-APB doses on intestinal ischemia-reperfusion injury in an experimental rat model. Methods We divided 24 Wistar albino rats into four groups: sham, control, ischemia-reperfusion +2 mg/kg 2-APB, and ischemia-reperfusion +4 mg/kg 2-APB. The sham group only underwent laparotomy for 1 h 30 min. A 30-min period of mesenteric ischemia was induced in the control and two treatment groups, followed by 1 h of reperfusion. Before the laparotomy, 2 mg/kg and 4 mg/kg 2-APB was administered i.v. in the treatments groups, and blood samples were collected after reperfusion. Serum levels of malondialdehyde, superoxide dismutase, glutathione, total antioxidant capacity, tumor necrosis factor (TNF)-alpha, and interleukin-6 were analyzed. Intestinal tissues were taken for histopathological, DNA fragmentation, and terminal deoxynucleotidyl transferase dUTP nick end labeling analyses to determine the proportion of apoptotic cells. Results 2-APB reduced serum malondialdehyde, TNF-alpha, and interleukin-6 levels. However, superoxide dismutase and total antioxidant capacity levels increased significantly in the 4-mg/kg 2-APB group (p < 0.05). The intestinal histopathological injury scores were significantly higher in the control group; these injuries were prevented in the 4-mg/kg 2-APB dose group. DNA damage after ischemia-perfusion decreased significantly in the 4-mg/kg 2-APB group compared with the control group. Conclusion 2-APB decreases oxidative stress and cell injury. Administering 4 mg/kg 2-APB prevented ischemia-perfusion injury by diminishing histological damage.
dc.identifier.doi10.1007/s10353-016-0452-y
dc.identifier.endpage37
dc.identifier.issn1682-8631
dc.identifier.issn1682-4016
dc.identifier.issue1
dc.identifier.startpage32
dc.identifier.urihttps://doi.org/10.1007/s10353-016-0452-y
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24538
dc.identifier.volume49
dc.identifier.wosWOS:000393820300007
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherSpringer Wien
dc.relation.ispartofEuropean Surgery-Acta Chirurgica Austriaca
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectIntestinal ischemia
dc.subject2-APB
dc.subjectIschemia-reperfusion injury
dc.titleEffect of different doses of 2aEuroaminoethoxydiphenyl borate on intestinal ischemia-reperfusion injury
dc.typeArticle

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