GENOTOXICITY ASSESSMENT OF HEAVY METALS (Zn, Cr, Pb) ON STRAWBERRY PLANTS USING RAPD ASSAY

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dc.authoridCEYLAN, Yusuf/0000-0001-8186-7252
dc.authoridCEYLAN, Kevser Betul/0000-0002-6006-727X
dc.contributor.authorSurgun-Acar, Yonca
dc.contributor.authorIskil, Rabia
dc.contributor.authorCeylan, Keyser Betul
dc.contributor.authorCeylan, Yusuf
dc.date.accessioned2025-01-27T20:12:20Z
dc.date.available2025-01-27T20:12:20Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractThe aim of the present study is to assess DNA damage in leaves of strawberry (Fragaria x ananassa Duch.) seedlings treated with different heavy metals (zinc, chromium, lead) using random amplified polymorphic DNA (RAPD) assay and analysis of total soluble protein content. For this purpose, strawberry seedlings were treated with 400 and 800 mu M Zn, Pb and Cr for 7 days. Thirty four RAPD primers produced 218 bands at molecular weight ranging from 183 bp to 5180 bp. Compared with control, RAPD patterns of heavy metal exposed groups showed differences in band loss, gain of new bands and increase and decrease of band intensity. The highest polymorphism rate (32.11%) was observed in 800 mu M Pb applied strawberry seedlings. To evaluate the alterations in RAPD profiles qualitatively, genomic template stability (GTS) was performed and the values were 75.08% and 69.59% for 400 and 800 mu M Zn treatments, 74.52% and 68.60% for 400 and 800 mu M Cr treatments, 70.11% and 63.78% for 400 and 800 mu M Pb treatments, respectively. Total soluble protein content in heavy metal-treated groups also showed a similar correlation to GTS values. RAPD analyses are useful biomarker assays to determine the genotoxicity induced by environmental pollutants such as heavy metals in plant model systems.
dc.description.sponsorshipBartin University Scientific Research Projects Unit [2016-FEN-A-015]
dc.description.sponsorshipThe authors would like to thank the Bartin University Scientific Research Projects Unit for their support (Project number: 2016-FEN-A-015). The authors also thank Dr. Hasan Ufuk Celebioglu for his kind contribution in English edition.
dc.identifier.endpage2491
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue4
dc.identifier.startpage2483
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20915
dc.identifier.volume27
dc.identifier.wosWOS:000430372900063
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherParlar Scientific Publications (P S P)
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectBiomarker
dc.subjectDNA damage
dc.subjectgenomic template stability
dc.subjecttotal soluble protein
dc.titleGENOTOXICITY ASSESSMENT OF HEAVY METALS (Zn, Cr, Pb) ON STRAWBERRY PLANTS USING RAPD ASSAY
dc.typeArticle

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