An application on fish bones by chemical modification of histidine as amino acid

In this study, fish bones as a waste in fish processing was investigated whether they could be used as multi-functional materials by chemical modification. Histidine was used and modified on the surface of bone particle by esterification method. The reaction was carried out in water. The results showed that the surface modification of bone particles was performed successfully. Histidine bonded on the surface of bone was calculated as 110.83 µmol/g. The point of zero charge (PZC) of bone apatite (H) and modified bone (HA4) was investigated and determined as 7.25 and 6.98, respectively. SEM-EDX spectrums showed that nitrogen element of histidine on the surface of HA4 could be observed in EDS spectrum clearly. According to EDS spectrum analysis, nitrogen amount of HA4 which was formed to the one of histidine composition was detected as 16.2 %., nitrogen amount of Hand HA5 was detected as 8.037% and 8.565 %, respectively.