The effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1?) expression in rabbit cornea

Basit Öğe Kaydı
dc.contributor.authorErtekin, Yusuf Haydar
dc.contributor.authorArıkan, Sedat
dc.contributor.authorKaraboğa, İhsan
dc.date.accessioned2025-01-27T20:01:31Z
dc.date.available2025-01-27T20:01:31Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIntroduction: To investigate the effect of ozone (O3) therapy on cornea subjected to systemically used high dose methylprednisolone (MP) ina rabbit model.Methods: Twenty-four New Zealand White adult male rabbits were randomly divided into three equal groups as containing eight animals. Thefirst group (n = 8) was used as the control group and nothing was applied to them, whereas the other 2 groups named as steroid groups weresubjected to IM methylprednisolone injection at a dose of 20 mg/kg/day for three days. After three days of MP administration, only the third groupwas treated with 50-µg/mL O3 (20 mL O3) through the rectal insufflation for 14 sessions. The histopathological examination of corneas of threegroups were made, and they were also assessed regarding the expression of hypoxia-inducible factor-1 ? (HIF-1?).Results: It was determined that systemically administered high dose MP caused erosion and necrosis in corneal epithelium and stromaldisintegrations in corneal stroma in steroid groups (Group 2 and Group 3). In the MP + O3 group (Group 3), the histopathological findings weremild. The expression of HIF1-? in the cornea of Group1 (control group), Group 2 (MP), and Group 3 (MP-O3) was measured as, 17.9±9.6%,3.1±1.0% and 6.4±1.9% respectively.Conclusions: MP and MP-O3 therapy decreased HIF-1a expression in rabbit cornea in both intervention groups. Between these two groups,HIF-1? expression remained relatively high in the MP-O3 group than in the MP group alone.
dc.identifier.endpage129
dc.identifier.issn2458-8865
dc.identifier.issn2459-1505
dc.identifier.issue3
dc.identifier.startpage126
dc.identifier.trdizinid372064
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/372064
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20121
dc.identifier.volume4
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofAile Hekimliği ve Palyatif Bakım
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectGenel ve Dahili Tıp
dc.subjectBiyokimya ve Moleküler Biyoloji
dc.titleThe effect of ozone (O3) therapy in addition to high dose methylprednisolone on hypoxia inducible factor-1 alpha (HIF-1?) expression in rabbit cornea
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu