Molecular identification of adenoviral conjunctivitis in Turkey

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dc.contributor.authorYa?ci, Ramazan
dc.contributor.authorAkçali, Alper
dc.contributor.authorYa?ci, Server
dc.contributor.authorKonno, Tsunetada
dc.contributor.authorIshiko, Hiroaki
dc.contributor.authorDuman, Sunay
dc.contributor.authorÖzkaya, Etem
dc.date.accessioned2025-01-27T18:56:12Z
dc.date.available2025-01-27T18:56:12Z
dc.date.issued2010
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractPURPOSE. The aim of the study was isolation of adenoviruses by cell culture and identification using polymerase chain reaction (PCR) and phylogenetic analyses in patients clinically diagnosed with viral conjunctivitis in Ankara, Turkey. METHODS. Conjunctival swabs from 34 patients with acute conjunctivitis were tested using cell culture isolation and PCR for adenovirus detection. PCR-positive samples were sequenced and typed. RESULTS. The positive results of adenovirus were 26.5% (9 of 34) by the PCR method and 20.6% by culture isolation. Nine samples positive at PCR were identified by phylogenetic analyses as human adenovirus 8 (HAdV-8) (4 of 9), HAdV-3 (3 of 9), HAdV-4 (1 of 9), and HAdV-B (1 of 9). CONCLUSIONS. Our study showed types of adenoviruses in patients with ocular infection that occurred in this region of Turkey for the first time. Furthermore, sequence-based typing method is an efficient, accurate, and rapid means of diagnosis and typing of the adenovirus and has significant clinical and epidemiologic implications. HAdV-8 was major type for acute conjunctivitis in Ankara, Turkey. Further studies are required to reveal the major types of HAdVs that cause ocular diseases in this region of the world. © 2010 Wichtig Editore.
dc.identifier.doi10.1177/112067211002000404
dc.identifier.endpage674
dc.identifier.issn1120-6721
dc.identifier.issue4
dc.identifier.pmid20037907
dc.identifier.scopus2-s2.0-77955836005
dc.identifier.scopusqualityQ2
dc.identifier.startpage669
dc.identifier.urihttps://doi.org/10.1177/112067211002000404
dc.identifier.urihttps://hdl.handle.net/20.500.12428/12898
dc.identifier.volume20
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWichtig Publishing Srl
dc.relation.ispartofEuropean Journal of Ophthalmology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_Scopus_20250125
dc.subjectAdenovirus; Conjunctivitis; Molecular identification; Turkey
dc.titleMolecular identification of adenoviral conjunctivitis in Turkey
dc.typeArticle

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