Double and Triple Gel Stocks Method in Pulsed Field Gel Electrophoresis

Although pulsed-field gel electrophoresis (PFGE) is a very valuable and gold standard method in molecular typing of bacteria-based epidemic diseases, its main disadvantages are as follows; it is time-consuming, it enables simultaneous working on a very limited samples and it requires experienced personnel. This study aims to work on 2-3 times more samples during the single gel system protocol simultaneously with the application of double and triple gel system to current PFGE system. In order to ensure the perfection of DNA patterns observed in gels to be assessed as those in single system and to prevent the contamination of these DNA patterns; intermediate lamellas manufactured from 13x14 cm polystyrene (PS), acrylonitrile-butadiene-styrene (ABS) and acetyl cellulose were used. The results obtained from the double gel system including polystyrene (PS), acrylonitrile-butadiene-styrene(ABS) are similar with the original gel system without sacrificing quality. However the desired results could not be obtained from the triple gel system in which the intermediate lamella made of acetyl cellulose was used.