Investigation of Isozyme Polymorphism and Nuclear DNA Content Variation of Free-Pollinated Peach (Prunus persica L.) Seedlings

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dc.contributor.authorSeker, M.
dc.date.accessioned2025-01-27T20:31:21Z
dc.date.available2025-01-27T20:31:21Z
dc.date.issued2012
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description7th International Peach Symposium -- JUN 08-11, 2009 -- Lleida, SPAIN
dc.description.abstractMost of peach and nectarine cultivars are propagated traditionally on seedling rootstocks in Turkey. For this purpose, 'Uzunoglu' peach [Prunus persica L. (Batsch)] has been used extensively for long years. The fruits of 'Uzunoglu' peach are having no commercial value except good rootstock characteristics. The objectives of this study were to compare the isozyme polymorphism and nuclear DNA content variation in free-pollinated peach seedling populations. During the study, isozyme variation was surveyed for five enzymes including alcohol dehydrogenase (ADH, EC 1.1.1), isocitrate dehydrogenase (IDH, EC 1.1.1.42), malate dehydrogenase (MDH, EC 1.11.1.37), peroxidase (PRX, EC 1.11.1.7) and phosphogluco mutase (PGM, EC 2.7.5.1) in the seedlings. Moreover, genome size (C-value) was measured in peach seedling rootstocks. Cell nuclei were isolated from leaf tissues of the seedlings and then fluorescence intensities were measured on propidium iodide stained nuclei by using 'Tahiti' lime [Citrus latifolia Tan.] and mungbean [Vigna radiata 'Berken'] as internal reference standard by flow cytometry. According to the obtained results, isozyme variation was detected in 5 isozyme systems resulting 7 loci and 13 alleles. Isozyme polymorphism has only been observed in malate dehydrogenase profiles. Results obtained from flow cytometric analysis indicated that all analyzed seedlings were found to be diploid genomes with 0.574 pg/2C value in average. The study demonstrates the value of clonal rootstocks for eliminating of genetic variability within seedling rootstocks.
dc.description.sponsorshipInt Soc Hort Sci (ISHS)
dc.identifier.endpage183
dc.identifier.isbn978-90-66056-15-2
dc.identifier.issn0567-7572
dc.identifier.issn2406-6168
dc.identifier.startpage177
dc.identifier.urihttps://hdl.handle.net/20.500.12428/23110
dc.identifier.volume962
dc.identifier.wosWOS:000323610100026
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherInt Soc Horticultural Science
dc.relation.ispartofVii International Peach Symposium
dc.relation.publicationcategoryKonferans Öğesi - Uluslararası - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectpeach
dc.subjectseedling
dc.subjectrootstock
dc.subjectisozyme
dc.subjectflow cytometry
dc.subjectC-value
dc.subjectvariation
dc.titleInvestigation of Isozyme Polymorphism and Nuclear DNA Content Variation of Free-Pollinated Peach (Prunus persica L.) Seedlings
dc.typeConference Object

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