The diagnostic accuracy of non-invasive fetal RhD genotyping by using cell-free fetal DNA in maternal plasma

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dc.contributor.authorAkurut, Cisem
dc.contributor.authorSılan, Fatma
dc.contributor.authorYalçıntepe, Sinem
dc.contributor.authorÖzdemir, Öztürk
dc.date.accessioned2025-01-27T20:01:29Z
dc.date.available2025-01-27T20:01:29Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIntroduction: The non-invasive prenatal diagnosis of the fetus RhD genotype in RhD incompatibility has a crucial role in the prevention ofincreased anti-D immunoglobulin therapy for haemolytic diseases in pregnant women carrying RhD negative fetus. It was aimed to detect fetalRhD genotyping by using maternal circulating cell-free DNA in the current study.Methods: Maternal blood samples were collected in different trimester of pregnancies (12-40 weeks) in 12 D-negative mothers. Cell-free fetalDNA was extracted from 2 ml of maternal plasma by an conventional DNA isolation technique (Qiagen, Hilden, Germany) and real-time PCRwas performed for genotyping target RhD exons 7 and 10 and GLO genes. Postnatal serological evaluations were performed and the results wereconfirmed.Results: 6 cases (50 %) were determined D positive and 6 cases (50 %) were determined D negative. All results were also confirmed after birthserologically.Conclusions: In conclusion, the current results showed us the non-invasive target RhD genotyping from cell free fetal DNA from maternalplasma samples have a diagnostic accuracy in RhD incompatibility pregnancies.
dc.identifier.endpage6
dc.identifier.issn2458-8865
dc.identifier.issn2459-1505
dc.identifier.issue1
dc.identifier.startpage1
dc.identifier.trdizinid372010
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/372010
dc.identifier.urihttps://hdl.handle.net/20.500.12428/20107
dc.identifier.volume4
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofAile Hekimliği ve Palyatif Bakım
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectKadın Hastalıkları ve Doğum
dc.subjectHalk ve Çevre Sağlığı
dc.titleThe diagnostic accuracy of non-invasive fetal RhD genotyping by using cell-free fetal DNA in maternal plasma
dc.typeArticle

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