Investigation of extracellular protease activity of two different extremely halophilic archaea isolated from raw hide
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Tarih
2017
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
INCDTP: Division Leather Footwear Research Institute
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
In this study, it was aimed to investigate extracellular protease activity of two different extremely halophilic archaea and to identify them by using phenotypic and molecular methods. The haloarchaea were isolated from salted raw hide. The isolates were screened for production of protease enzyme and two isolates which have extracellular protease activity were selected. To identify the halophilic archaea, Gram staining procedure, antibiotic sensitivity tests and molecular methods were used. According to the 16S r-RNA molecular identifi cation results, isolate 1 identifi ed as Halococcus morrhuae JCM 8876 with ti99 sequence similarities and isolate 2 identifi ed as Natrinema pallidum 153 with %99 sequence similarities. Azocasein was used as a substrate for measuring their proteases activities daily and their optimum activities were determined. H. morrhuae and N. pallidum showed daily maximum proteases activity on 6 and 4 days, respectively. According to the results of the study, it was found that proteases activity was related with different reaction conditions. These conditions were detected as different pH (4-11), temperature (4-70°C) and NaCl concentration (1-5 M) values. H. morrhuae showed the maximum activity at pH 9.5, 2M NaCl concentration and 50°C temperature and N. pallidum displayed the optimum activity at pH 9.0, 2M NaCl concentration and 40°C temperature. © 2017 by the author(s).
Açıklama
Anahtar Kelimeler
16S r-RNA sequence analysis; Extracellular protease enzyme; Haloarchaea; Salted hide
Kaynak
Leather and Footwear Journal
WoS Q Değeri
Scopus Q Değeri
Q4
Cilt
17
Sayı
4
