GENETIC DIVERSITY OF TURKISH CITRUS CHLOROTIC DWARF-ASSOCIATED VIRUS ISOLATES BASED ON COAT PROTEIN GENE

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dc.contributor.authorKaranfil, Ali
dc.contributor.authorKorkmaz, Savaş
dc.date.accessioned2025-01-27T20:45:42Z
dc.date.available2025-01-27T20:45:42Z
dc.date.issued2018
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractCitrus chlorotic dwarf-associated virus (CCDaV) emerged in Turkey in the 1980s. It has just recently been identified and characterized. Studies concerning genetic diversity of CCDaV are very limited. This study was designed to undertake a molecular characterization of Turkish CCDaV isolates. In this study, twenty-three CCDaV isolates obtained from different citrus species within the Eastern Mediterranean Region of Turkey (in which the first CCDaV in the world was discovered) were used for molecular characterization based on coat protein (CP) genes of CCDaV isolates. For this purpose, the CP gene from CCDaV isolates was amplified and cloned. The cloned CP gene of CCDaV isolates was sequenced bidirectionally. The resulting sequences were assembled and deposited in Genbank. Similarity rates and phylogenetic relationships in Turkish CCDaV isolates, including the limited number of CCDaV isolates found in Genbank, were further researched. As a result of the multiple sequence analysis, it was determined that Turkish CCDaV isolates showed over 98% nucleotide- and amino acid-based similarities with each other and other worldwide CCDaV isolates. In addition, as a result of phylogenetic analyses, it was determined that Turkish and Chinese isolates had a tendency to split apart from each other based on geographic origin. However, it was shown that there were no phylogenetically specific clusters in the Turkish CCDaV isolates similar to the host citrus species.
dc.description.sponsorshipScientific and Technological Research Council of Turkey [TUBITAK - TOVAG 117O548]
dc.description.sponsorshipThis research is a part of the first author's Ph.D. thesis and supported by a grant from Scientific and Technological Research Council of Turkey (TUBITAK - TOVAG 117O548). We thank Prof. Dr. Bayram Cevik for his assistance in this study.
dc.identifier.endpage6407
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue9
dc.identifier.startpage6403
dc.identifier.urihttps://hdl.handle.net/20.500.12428/24686
dc.identifier.volume27
dc.identifier.wosWOS:000446644900077
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherParlar Scientific Publications (P S P)
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectPCR
dc.subjectCloning
dc.subjectSimilarity
dc.subjectPhylogenetic
dc.titleGENETIC DIVERSITY OF TURKISH CITRUS CHLOROTIC DWARF-ASSOCIATED VIRUS ISOLATES BASED ON COAT PROTEIN GENE
dc.typeArticle

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