Type II collagen antibody response is enriched in the synovial fluid of rheumatoid joints and directed to the same major epitopes as in collagen induced arthritis in primates and mice

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dc.contributor.authorLindh, Ingrid
dc.contributor.authorSnir, Omri
dc.contributor.authorLönnblom, Erik
dc.contributor.authorUysal, Hüseyin
dc.contributor.authorAndersson, Ida
dc.contributor.authorNandakumar, Kutty S.
dc.contributor.authorVierboom, Michel
dc.date.accessioned2025-01-27T18:56:12Z
dc.date.available2025-01-27T18:56:12Z
dc.date.issued2014
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIntroduction: Antibodies towards type II collagen (CII) are detected in patients with rheumatoid arthritis (RA) and in non-human primates and rodents with collagen induced arthritis (CIA). We have previously shown that antibodies specific for several CII-epitopes are pathogenic using monoclonal antibodies from arthritic mice, although the role of different anti-CII epitopes has not been investigated in detail in other species. We therefore performed an inter-species comparative study of the autoantibody response to CII in patients with RA versus monkeys and mice with CIA.Methods: Analysis of the full epitope repertoire along the disease course of CIA was performed using a library of CII triple-helical peptides. The antibody responses to the major CII epitopes were analyzed in sera and synovial fluid from RA patients, and in sera from rhesus monkeys (Macaca mulatta), common marmosets (Callithrix jacchus) and mice.Results: Many CII epitopes including the major C1, U1, and J1 were associated with established CIA and arginine residues played an important role in the anti-CII antibody interactions. The major epitopes were also recognized in RA patients, both in sera and even more pronounced in synovial fluid: 77% of the patients had antibodies to the U1 epitope. The anti-CII immune response was not restricted to the anti-citrulline protein antibodies (ACPA) positive RA group.Conclusion: CII conformational dependent antibody responses are common in RA and are likely to originate from rheumatoid joints but did not show a correlation with ACPA response. Importantly, the fine specificity of the anti-CII response is similar with CIA in monkeys and rodents where the recognized epitopes are conserved and have a major pathogenic role. Thus, anti-CII antibodies may both contribute to, as well as be the consequence of, local joint inflammation. © 2014 Lindh et al.; licensee BioMed Central Ltd.
dc.description.sponsorshipKA Wallenberg foundation; King Gustav V 80-years foundation; Swedish Science Strategic Foundation; Seventh Framework Programme, FP7, (223404); European Commission, EC, (HEALTH-F2-2008-223404); Stiftelsen för Strategisk Forskning, SSF; Vetenskapsrådet, VR; Reumatikerförbundet
dc.identifier.doi10.1186/ar4605
dc.identifier.issn1478-6354
dc.identifier.issue4
dc.identifier.pmid25005029
dc.identifier.scopus2-s2.0-84903808546
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1186/ar4605
dc.identifier.urihttps://hdl.handle.net/20.500.12428/12901
dc.identifier.volume16
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBioMed Central Ltd.
dc.relation.ispartofArthritis Research and Therapy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_Scopus_20250125
dc.subjectAdult; Aged; Aged, 80 and over; Animals; Antibody Formation; Arthritis, Experimental; Arthritis, Rheumatoid; Autoantibodies; Autoantigens; Callithrix; Collagen Type II; Epitopes, B-Lymphocyte; Female; Humans; Macaca mulatta; Male; Mice; Mice, Inbred BALB C; Middle Aged; Synovial Fluid; Young Adult; arginine; citrulline protein antibody; collagen antibody; collagen type 2; collagen type 2 antibody; epitope; HLA DRB1 antigen; protein antibody; unclassified drug; autoantibody; autoantigen; collagen type 2; epitope; adult; aged; animal experiment; animal tissue; antibody blood level; antibody library; antibody response; antibody specificity; antibody titer; antigen binding; arthritis; Article; Callithrix; comparative study; controlled study; correlation analysis; disease course; epitope mapping; female; human; human tissue; immune response; male; mouse; nonhuman; protein conformation; protein expression; protein interaction; rhesus monkey; rheumatoid arthritis; synovial fluid; synovial fluid level; animal; antibody production; Bagg albino mouse; experimental arthritis; immunology; middle aged; synovial fluid; very elderly; young adult
dc.titleType II collagen antibody response is enriched in the synovial fluid of rheumatoid joints and directed to the same major epitopes as in collagen induced arthritis in primates and mice
dc.typeArticle

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