Purification of buchnericin LB produced by Lactobacillus buchneri LB

Basit Öğe Kaydı
dc.contributor.authorYıldırım, Metin
dc.date.accessioned2025-01-27T19:57:49Z
dc.date.available2025-01-27T19:57:49Z
dc.date.issued2001
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractBuchnericin LB produced by Lactobacillus buchneri LB was purified to homogeneity by a rapid and simple three-step purification procedure including freeze drying, silicic acid adsorption/desorption and cation-exchange chromatography. After the silicic acid and cation-exchange chromatography steps, the activity of buchnericin LB was recovered by 85 and 25%, and its purity increased about 111 and 2,500 fold, respectively. It was determined that the adsorption of buchnericin LB to silicic acid and cation-exchange chromatography was dependent on the pH of the suspending environment. The molecular weight of buchnericin LB was determined to be about 4.0 kDa by tricine SDS-PAGE.
dc.identifier.endpage65
dc.identifier.issn1300-0152
dc.identifier.issn1303-6092
dc.identifier.issue1
dc.identifier.startpage59
dc.identifier.trdizinid27779
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/27779
dc.identifier.urihttps://hdl.handle.net/20.500.12428/19750
dc.identifier.volume25
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTurkish Journal of Biology
dc.relation.publicationcategoryDiğer
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectBiyoloji
dc.subjectMikrobiyoloji
dc.titlePurification of buchnericin LB produced by Lactobacillus buchneri LB
dc.typeOther

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu