Hplc Analysis of Certain Phenolic Compounds and Carotenoid in Two Calendula Species
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Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Calendula officinalis (pot marigold), a medicinal ornamental plant belonging to the Asteraceaefamily, has various medicinal activities such as anticancer, antimicrobial, antioxidant and antitumor.The retention time of quercetin (6.445 min), kaempferol (11.246 min), caffeic acid (2.333 min), betacarotene (9.614 min) standards, which were planned to be analyzed by HPLC, was determined. TheHPLC conditions that are most suitable for these metabolites have been determined. The amounts ofquercetin, kaempferol, caffeic acid and beta carotene in the seedling leaves of these two Calendulaspecies on the 0., 15., 30., 45., 60. days were compared. Caffeic acid and beta carotene have beendetected in the leaves. However, quercetin and kaempferol compounds were not detected. It wasdetermined that the caffeic acid and beta carotene amounts of C. officinalis species were higher thanthat of C. arvensis. The highest caffeic acid in both C. officinalis and C. arvensis seedlings(0.4042±0.0123 µg/g dry weight for C. officinalis, 0.1918±0.0437 µg / g dry weight for C. arvensis)and beta carotene amounts (0.8520±0.0692 µg/g dry weight for C. officinalis, 0.6389±0.0189 µg/gdry weight for C. arvensis) were determined on 60. day. In addition, it was determined that the amountof these metabolites differed according to the seedling development period.
Açıklama
Anahtar Kelimeler
Biyoloji, Kimya, Uygulamalı, Bitki Bilimleri
Kaynak
Türk Tarım - Gıda Bilim ve Teknoloji dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
8
Sayı
12