THE EFFECTS OF VITAMIN E ON PERIODIC ACID SCHIFF REACTION, QUANTITY OF ARGYROPHILIC NUCLEOLAR ORGANIZER REGIONS AND INDUCIBLE NITRIC OXIDE SYNTHASE EXPRESSION IN PROSTATE OF RATS EXPOSED TO BISPHENOL A

The aim of the study is to determine the protective role of vitamin E on the prostate of rats exposed to bisphenol A (BPA). A total of 40 adult male Wistar Albino rats were used. The rats were randomly divided into five groups as follows: control, sham, vitamin E, BPA, and vitamin E+BPA. The applications of vitamin E (300 IU/kg/day) and BPA (10 mg/kg/day) were proceeded for three weeks. Periodic Acid Schiff (PAS) and Silver-Staining for Nucleolar Organizer Region (AgNOR) methods were applied to prostate sections for histochemical examinations. Also, inducible nitric oxide synthase (iNOS) expression was examined immunohistochemically. The number of AgNOR positive dots and intensity of iNOS expression in glandular epithelial cells of prostate increased in BPA group compared to control group. Also, iNOS expression decreased in vitamin E+BPA group compared to BPA group. On the other hand, a significant difference wasn't found between the groups in terms of PAS reaction. As a conclusion, our findings demonstrate that BPA might stimulate cell proliferation and oxidative changes in prostate. The usage of vitamin E might contribute to reducing oxidative damage induced by BPA in prostate.