Investigation of Isozyme Polymorphism in Open-Pollinated Sweet Cherry and 'Mahaleb' Seedlings
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Tarih
2008
Yazarlar
Seker, M.
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
International Society Horticultural Science
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
Traditionally, sweet cherry (Prunus avium L.) cultivars are propagated on seedling rootstocks in Turkey. For this purpose, wild cherries (P. avium) and 'Mahaleb' (P. mahaleb L.) have been used extensively. The use of clonal (vegetatively propagated) rootstocks for sweet cherries is low in Turkey due to the high cost of nursery plants. Seeds for rootstock propagation usually are collected by nurserymen from trees of unknown origin. The objectives of this study were to compare the seedlings of two Prunus species for morphological characteristics and to detect any isozyme polymorphisms in seedling populations. During the study, variations in germination rate, leaf area and seedling size were evaluated. Isozyme variation was surveyed for five enzymes including alcohol dehydrogenase (ADH, EC 1.1.1), isocitrate dehydrogenase (IDH, EC 1.1.1.42), malate dehydrogenase (MDH, EC 1.11.1.37), peroxidase (PRX, EC 1.11.1.7) and phosphoglucomutase (PGM, EC 2.7.5.1) in the populations. Wild cherry (P. avium) seedlings exhibited greater variation than P. mahaleb. Cherry seedlings had leaf areas ranging from 25.4 to 60.3 cm(2) lsozyme polymorphism was observed in seedling populations of both species at 7 enzyme loci with 35 alleles in total (19 alleles in cherries and 16 alleles in 'Mahaleb'). Isozyme variability was higher in sweet cherries than 'Mahaleb' due to high level of heterozygosity. The study demonstrates the value of clonal rootstocks for eliminating the genetic variability within seedling rootstocks.
Açıklama
5th International Cherry Symposium -- JUN 06-10, 2005 -- Bursa, TURKEY
Anahtar Kelimeler
Prunus avium, P. mahaleb, propagation, rootstock, genotypic variation
Kaynak
Proceedings of The Vth International Cherry Symposium, Vols 1 and 2
WoS Q Değeri
N/A
Scopus Q Değeri
Cilt
Sayı
795
