DETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS

Basit Öğe Kaydı
dc.contributor.authorHürkan, Kaan
dc.contributor.authorYüksel, Merve Ballı
dc.contributor.authorHürkan, Yasemin Kemeç
dc.contributor.authorManav, Neslihan Demir
dc.date.accessioned2025-01-27T19:22:35Z
dc.date.available2025-01-27T19:22:35Z
dc.date.issued2019
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractIn this study we evaluated the secondary metabolites, total phenolic (TPC) and flavonoid contents (TFC), antioxidant andantimicrobial activities of salep orchids, Anacamptis morio, Anacamptis pyramidalis, Neotinea tridentata, Ophrys mammosa,Ophrys lutea, and Ophrys speculum. DPPH free radical scavenging assay was used to determine the antioxidant activities ofn-hexane, chloroform, methanol and water extracts of the plants. The antimicrobial activities were also determined by the Brothmicro-dilution method. The extracts were studied for antimicrobial activity by the Minimum Inhibitory Concentration (MIC)approach against seven clinical pathogenic bacteria and two fungi. Phytochemical screening revealed that the presences ofcoumarins, flavonoids, flavanones, cardiac glycosides, proteins and quinones. The extracts had variable TPC and TFC, withvalues of 4.46 ± 0.19–45.83 ± 1.86 mg gallic acid equivalent/g dry weight and 0.67 ± 0.04–8.64 ± 0.37 mg quercetinequivalent/g dry weight respectively. O. speculum had the highest (35.12%) antioxidant activity, followed by O. mammosa(33.17%). Chloroform extracts of all species showed significant antioxidant and antimicrobial activity. These bioactivities ofthe chloroform extracts were positively associated with the total phenolic and flavonoid contents. The MIC concentrationsranged from 0.156–20 mg/mL. The present investigation shows that the extracts of these species, especially chloroformextracts, could be used as potential antioxidant and antimicrobial sources.
dc.identifier.doi10.18036/estubtdc.598852
dc.identifier.endpage202
dc.identifier.issn2667-4203
dc.identifier.issue2
dc.identifier.startpage191
dc.identifier.trdizinid390482
dc.identifier.urihttps://doi.org/10.18036/estubtdc.598852
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/390482
dc.identifier.urihttps://hdl.handle.net/20.500.12428/15130
dc.identifier.volume8
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofEskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online)
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectBiyoloji
dc.subjectKimya
dc.subjectTıbbi
dc.subjectBiyoteknoloji ve Uygulamalı Mikrobiyoloji
dc.titleDETERMINATION OF TOTAL PHENOLIC AND FLAVONOID CONTENTS, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF SOME IMPORTANT SALEP ORCHIDS
dc.typeArticle

Dosyalar

Koleksiyon

TR-Dizin İndeksli Yayınlar Koleksiyonu