Diversity Analysis of Common Vetch (Vicia Sativa L.) Lines and Cultivars Using Pairwise Combinations of Universal Rice Primers

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dc.contributor.authorTopu, Mustafa
dc.contributor.authorTiryaki, İskender
dc.date.accessioned2025-05-29T05:25:30Z
dc.date.available2025-05-29T05:25:30Z
dc.date.issued2022
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.descriptionKSU, BAP Grant #: 2009/2-3
dc.description.abstractThis study has been conducted to determine genetic diversity of the common vetch lines and cultivars by using pairwise combinations of universal rice primers (URPs). A total number of 37 URP marker pairs were tested and twenty of those provided amplicons in the common vetch genome. The pairs of amplified URP markers provided a total of 83 bands and 62 of them were determined as polymorphic and were scattered to the whole genome. The average polymorphism rate of the primers was calculated as 73.5% while the polymorphism information content (PIC) values have ranged from 0.11 to 0.47 with an average of 0.24. The phylogenetic tree constructed based on UPGMA analysis provided three main clades. Two-dimensional plot of PCA and the UPGMA analysis showed that the URP markers successfully distinguished the genetic material based on their genetic origin. In conclusion, this study revealed that the use of pairwise combinations of URP markers could have a better power to reveal the level of polymorphism in plant genome.
dc.description.sponsorshipKahramanmaras Sutcu Imam University
dc.identifier.doi10.38001/ijlsb.1122987
dc.identifier.endpage518
dc.identifier.issn2651-4621
dc.identifier.issue3
dc.identifier.startpage504
dc.identifier.urihttps://doi.org/10.38001/ijlsb.1122987
dc.identifier.urihttps://hdl.handle.net/20.500.12428/31317
dc.identifier.volume5
dc.language.isoen
dc.publisherInternational Society of Academicians
dc.relation.ispartofInternational Journal of Life Sciences and Biotechnology
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_DergiPark_20250529
dc.subjectCommon vetch
dc.subjectgenetic diversity
dc.subjectmolecular characterization
dc.subjectURP
dc.titleDiversity Analysis of Common Vetch (Vicia Sativa L.) Lines and Cultivars Using Pairwise Combinations of Universal Rice Primers
dc.typeResearch Article

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