PH-sensitive chitosan films for baker's yeast immobilization

dc.authoridSaraydin, Dursun/0000-0001-7191-510X
dc.contributor.authorÖztop, HN
dc.contributor.authorSaraydin, D
dc.contributor.authorÇetinus, S
dc.date.accessioned2025-01-27T20:16:30Z
dc.date.available2025-01-27T20:16:30Z
dc.date.issued2002
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractDried baker's yeast cells were immobilized on a chitosan film, which is a natural polymer. Prepared chitosan films were treated with glutaraldehyde to facilitate the immobilization of the cells. The effects of the amount of glutaraldehyde, incubation time, pH, and temperature on immobilization were investigated. The amount of glutaraldehyde was chosen to be 0.01% (weight). The highest amount of yeast immobilization was obtained with 5 h incubation. It was determined that optimum temperature for immobilization is 25degreesC, and the optimum pH for immobilization is 6. Immobilized cells were allowed to stand for 3 d in distilled water and buffer solution (pH 6) to investigate the desorption, but no desorption was found. The maximum immobilization capacities were found to be 90 mug protein cm(-2) film in optimum conditions.
dc.identifier.doi10.1385/ABAB:101:3:239
dc.identifier.endpage249
dc.identifier.issn0273-2289
dc.identifier.issue3
dc.identifier.pmid12109819
dc.identifier.startpage239
dc.identifier.urihttps://doi.org/10.1385/ABAB:101:3:239
dc.identifier.urihttps://hdl.handle.net/20.500.12428/21260
dc.identifier.volume101
dc.identifier.wosWOS:000176446500005
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherHumana Press Inc
dc.relation.ispartofApplied Biochemistry and Biotechnology
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WoS_20250125
dc.subjectyeast
dc.subjectcell
dc.subjectimmobilization
dc.subjectchitosan
dc.titlePH-sensitive chitosan films for baker's yeast immobilization
dc.typeArticle

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