Investigation of isozyme polymorphism in open-pollinated sweet cherry and 'Mahaleb' seedlings

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Tarih

2008

Yazarlar

Dergi Başlığı

Dergi ISSN

Cilt Başlığı

Yayıncı

International Society for Horticultural Science

Erişim Hakkı

info:eu-repo/semantics/closedAccess

Özet

Traditionally, sweet cherry (Prunus avium L.) cultivars are propagated on seedling rootstocks in Turkey. For this purpose, wild cherries (P. avium) and 'Mahaleb' (P. mahaleb L.) have been used extensively. The use of clonal (vegetatively propagated) rootstocks for sweet cherries is low in Turkey due to the high cost of nursery plants. Seeds for rootstock propagation usually are collected by nurserymen from trees of unknown origin. The objectives of this study were to compare the seedlings of two Prunus species for morphological characteristics and to detect any isozyme polymorphisms in seedling populations. During the study, variations in germination rate, leaf area and seedling size were evaluated. Isozyme variation was surveyed for five enzymes including alcohol dehydrogenase (ADH, EC 1.1.1), isocitrate dehydrogenase (IDH, EC 1.1.1.42), malate dehydrogenase (MDH, EC 1.11.1.37), peroxidase (PRX, EC 1.11.1.7) and phosphoglucomutase (PGM, EC 2.7.5.1) in the populations. Wild cherry (P. avium) seedlings exhibited greater variation than P. mahaleb. Cherry seedlings had leaf areas ranging from 25.4 to 60.3 cm2. Isozyme polymorphism was observed in seedling populations of both species at 7 enzyme loci with 35 alleles in total (19 alleles in cherries and 16 alleles in 'Mahaleb'). Isozyme variability was higher in sweet cherries than 'Mahaleb' due to high level of heterozygosity. The study demonstrates the value of clonal rootstocks for eliminating the genetic variability within seedling rootstocks.

Açıklama

Ataturk Central Horticultural Research Institute of Turkey; International Society for Horticultural Science; Scientific and Technological Research Council of Turkey; Turkish Society for Horticultural Science; Uludag University
5th International Cherry Symposium -- 6 June 2005 through 10 June 2005 -- Bursa --

Anahtar Kelimeler

Genotypic variation; P. mahaleb; Propagation; Prunus avium; Rootstock

Kaynak

Acta Horticulturae

WoS Q Değeri

Scopus Q Değeri

Q4

Cilt

795 PART 1

Sayı

Künye