Olive Leaf Extract-Induced Changes in Phenoloxidase Activity of Galleria mellonella (Lepidoptera: Pyralidae) Hemolmyph

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dc.contributor.authorKaya, Serhat
dc.contributor.authorTürkdoğan, Seranay
dc.date.accessioned2025-01-27T19:30:42Z
dc.date.available2025-01-27T19:30:42Z
dc.date.issued2021
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractSince the fruits and leaves of the Olive (Olea europea) tree, which grows naturally in Mediterraneancountries, are thought to be beneficial for many diseases, it is widely used in folk medicine in these countries.Olive leaf extract has been used in the treatment of many diseases such as malaria, high fever, heart diseasesand cancer. The greater wax moth Galleria mellonella (Lepidoptera: Pyralidae) is frequently used ininvertebrate immunity studies due to its similarity to the mammalian natural immune system. Melanizationoccurs when quinone precursors are converted to melanin and inactive profenoloxidase to phenoloxidaseenzyme. In this study, the phenoloxidase activity of G. mellonella hemolymph larvae injected with olive leafextract in different doses was determined by ELISA microplate reader at 492 nm absorbance. The findingsobtained from this study showed that olive leaf extract increased phenoloxidase activity at a dose of 0.001 mg$mL^{-1}$ . Lower doses of 0.001 mg $mL^{-1}$ did not cause changes in phenoloxidase activity compared to the untreatedand Dimethyl Sulfoxide (DMSO) groups. The highest olive leaf extract dose of our study is 0.01 mg $mL^{-1}$ and thelarvae did not survive for 24 hours over those doses. The results of our study show that olive leaf supportshumoral immune responses when used in low doses.
dc.identifier.doi10.30910/turkjans.897167
dc.identifier.endpage939
dc.identifier.issn2148-3647
dc.identifier.issue4
dc.identifier.startpage934
dc.identifier.trdizinid489274
dc.identifier.urihttps://doi.org/10.30910/turkjans.897167
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/489274
dc.identifier.urihttps://hdl.handle.net/20.500.12428/16227
dc.identifier.volume8
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.relation.ispartofTürk Tarım ve Doğa Bilimleri Dergisi
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_TRD_20250125
dc.subjectBiyoloji
dc.subjectEntomoloji
dc.titleOlive Leaf Extract-Induced Changes in Phenoloxidase Activity of Galleria mellonella (Lepidoptera: Pyralidae) Hemolmyph
dc.typeArticle

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