Internal transcribed spacer (ITS) sequence-based identification of yeast biota on pomegranate surface and determination of extracellular enzyme profile

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dc.authoridGUNAY, Melih/0000-0003-0336-3010
dc.contributor.authorGenc, Tulay Turgut
dc.contributor.authorGunay, Melih
dc.date.accessioned2025-01-27T20:56:02Z
dc.date.available2025-01-27T20:56:02Z
dc.date.issued2020
dc.departmentÇanakkale Onsekiz Mart Üniversitesi
dc.description.abstractYeasts are the most significant organisms to produce fermented products from different types of fruits such as grape, strawberry and pomegranate. The native yeasts on these fruits contribute to beverages' quality and aroma during fermentation. Pomegranate is used in fruit juice and wine production because of high antioxidant characteristic. In order to determine yeast microbiota on the pomegranate fruits collected from Gallipoli (Gelibolu), Canakkale-Turkey, ITS-5.8S rDNA gene region have been utilized. Also, phylogenetic relationships among identified yeast species were assigned by using sequences of ITS-5.8S rDNA gene region. In addition, extracellular enzyme activity of identified yeast strains was detected by using API-ZYM. Kluyveromyces lactis, Aureobasidium pullulans, Hanseniaspora uvarum, Candida zeylanoides, Kwoniella sp., and Metschnikowia pulcherrima and Metschnikowia ziziphicola yeast species were identified on pomegranate surface. Phylogenetic analysis, carried out in ITS-5.8S rDNA gene region of identified yeast strains, revealed the presence of five clades. Kwoniella sp., H. uvarum, M. pulcherrima, and Kl. lactis yeast strains revealed high leucine arylamidase activity. Also valine arylamidase activity was determined in M. pulcherrima and Kl. lactis yeast species. Acid phosphatase activity was determined in H. uvarum and K. lactis yeast species. Uncultured Kwoniella sp. and H. uvarum yeast species displayed high beta-galactosidase and beta-glucosidase activities, respectively.
dc.description.sponsorshipCanakkale Onsekiz Mart University, Turkey trought The Scientific Research Coordination Unit [FYL-2014-110]
dc.description.sponsorshipThis work was supported by Canakkale Onsekiz Mart University, Turkey trought The Scientific Research Coordination Unit, Project number: FYL-2014-110. The authors declare that they have no conflict of interests.
dc.identifier.doi10.13057/nusbiosci/n120111
dc.identifier.endpage67
dc.identifier.issn2087-3948
dc.identifier.issn2087-3956
dc.identifier.issue1
dc.identifier.startpage59
dc.identifier.urihttps://doi.org/10.13057/nusbiosci/n120111
dc.identifier.urihttps://hdl.handle.net/20.500.12428/26283
dc.identifier.volume12
dc.identifier.wosWOS:000560662800011
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherUniv Sebelas Maret
dc.relation.ispartofNusantara Bioscience
dc.relation.publicationcategoryinfo:eu-repo/semantics/openAccess
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WoS_20250125
dc.subjectAPI-ZYM
dc.subjectITS
dc.subjectPCR-RFLP
dc.subjectpomegranate
dc.subjectyeast microbiota
dc.titleInternal transcribed spacer (ITS) sequence-based identification of yeast biota on pomegranate surface and determination of extracellular enzyme profile
dc.typeArticle

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