Yazar "Sandikci, Mustafa" seçeneğine göre listele

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    Changes in rat ovary with experimentally induced diabetes and the effects of lycopene on those changes
    (Editura Acad Romane, 2016) Yıldız, Mustafa; Sandikci, Mustafa
    Free radicals increase in the presence of diabetes. Lycopene is a powerful antioxidant. The goal of the present study was to determine the effect of diabetes on rat ovaries and the protective role of lycopene in that context. Experimental diabetes was induced with 50 mg/kg streptozotocin. Rats were randomly separated into four groups, as follows: control + corn oil, control + lycopene, diabetes + corn oil and diabetes + lycopene. The histological and histometric evaluations were performed using Crossman's triple staining method. The immunohistochemical connexin-43 expression was identified and the apoptotic cell density was determined using the terminal deoxynucleotidyl transferase dUTP nick-end labeling method, while the malondialdehyde levels were measured using the enzyme-linked immunosorbent assay technique in the ovaries. Vacuolization of the corpus luteum, hydropic degeneration in the interstitial regions, and the number of corpora lutea increased in the ovary as effects of diabetes while the diameter of the corpora lutea decreased. The intensity of connexin-43 expression decreased in the primordial and atretic follicles, interstitial cells and luteal cells of the corpora lutea in the diabetes + corn oil group. The ovarian malondialdehyde levels and the number of apoptotic cells in the granulose layers of the large antral follicles increased in the presence of diabetes. Lycopene increased the expression of connexin-43 in the primordial, secondary and large antral follicles in the ovaries of diabetic animals. The changes caused by diabetes in the ovaries and the protective role of lycopene in some but not all parameters was revealed.
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    Changes in the Pancreas in Experimental Diabetes and the Effect of Lycopene on These Changes: Proliferating, Apoptotic, and Estrogen Receptor a Positive Cells
    (Wiley, 2017) Sandikci, Mustafa; Karagenc, Levent; Yıldız, Mustafa
    This study aimed to investigate the changes occurring in estrogen receptor ( ER) alpha-positive cells, proliferating cells, apoptotic cells and malondialdehyde ( MDA) expression in the pancreas of experimentally induced adult diabetic rats and to determine the effect of orally administered lycopene on these changes. Experimental diabetes was induced using a single dose of 50 mg/kg streptozotocin ( STZ). Following the administration of STZ, four groups of animals were established: Control1corn oil, control1lycopene, diabetic1corn oil and diabetic1lycopene. The expressions of ER a, Ki-67, and MDA were determined through immunohistochemistry in sections taken from pancreas tissue samples at the end of the experiment. Apoptotic cells were determined through the TUNEL method. In the diabetic groups, the densities of ER a expression in islets and ER alpha-positive cells in exocrine parts increased. Whereas the number of proliferating Ki-67 positive cells was higher in the diabetic groups, no significant difference was observed in terms of apoptotic cell number between the control and diabetic groups. Lycopene in general did not have any effect on any of the parameters analyzed in the study. The presence of ER alpha-positive cells around the islets was demonstrated for the first time in diabetic groups. Based on these observations, demonstrating the antioxidant effects of lycopene in the pancreas of diabetic rats may be possible by increasing the dose and/or the duration of lycopene. Anat Rec, 300:2000-2007, 2017. (C) 2017 Wiley Periodicals, Inc.
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    CHANGES IN THE UTERUS AND VAGINA OF RATS WITH EXPERIMENTALLY INDUCED DIABETES AND THE EFFECT OF LYCOPENE ON THE CHANGES
    (Univ Ljubljana, Veterinary Faculty, 2016) Yıldız, Mustafa; Sandikci, Mustafa
    The aims of this study were to identify the changes that occurred in the uterus and vagina of rats with experimental diabetes, and the effects of orally administered lycopene on these changes. For this research, 42 four-month-old female Wistar Albino rats were used. Experimental diabetes was induced using a single dose of 50 mg/kg streptozotocin (STZ). The control and diabetic rats were randomly separated into four groups as follows: control+corn oil, control+ lycopene, diabetes+corn oil, and diabetes+lycopene. Crossman's triple staining and the Periodic Acid Schiff (PAS) methods were applied to the uterine and vaginal slides. Additionally, the uterus was stained with Alcian Blue (AB) pH: 2.5. While the number of glands, the thickness of the endometrium, and the PAS-positive reaction in the glandular epithelium were lower, the AB positive reaction in the glandular epithelium was higher in the diabetes+corn oil group in comparison with the control+ corn oil group in the uterus. In contrast, while the height of the lamina epithelialis and the thickness of the tunica muscularis were smaller in the diabetes+corn oil group, in comparison with the control+ corn oil group, the thickness of the tunica muscularis was larger in the diabetes+lycopene group, in comparison with the vagina of the diabetes+corn oil group. The changes caused by diabetes in the uterus and vagina, as well as the existence of the protective qualities of lycopene were revealed for the first time, to the best of our knowledge.
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    Öğe
    Changes in the uterus and vagina of rats with experimentally induced diabetes and the effect of lycopene on the changes
    (University of Ljubljana - Veterinary Faculty, 2016) Yıldız, Mustafa; Sandikci, Mustafa
    The aims of this study were to identify the changes that occurred in the uterus and vagina of rats with experimental diabetes, and the effects of orally administered lycopene on these changes. For this research, 42 four-month-old female Wistar Albino rats were used. Experimental diabetes was induced using a single dose of 50 mg/kg streptozotocin (STZ). The control and diabetic rats were randomly separated into four groups as follows: control+corn oil, control+lycopene, diabetes+corn oil, and diabetes+lycopene. Crossman's triple staining and the Periodic Acid Schiff (PAS) methods were applied to the uterine and vaginal slides. Additionally, the uterus was stained with Alcian Blue (AB) pH: 2.5. While the number of glands, the thickness of the endometrium, and the PAS-positive reaction in the glandular epithelium were lower, the AB positive reaction in the glandular epithelium was higher in the diabetes+corn oil group in comparison with the control+corn oil group in the uterus. In contrast, while the height of the lamina epithelialis and the thickness of the tunica muscularis were smaller in the diabetes+corn oil group, in comparison with the control+corn oil group, the thickness of the tunica muscularis was larger in the diabetes+lycopene group, in comparison with the vagina of the diabetes+corn oil group. The changes caused by diabetes in the uterus and vagina, as well as the existence of the protective qualities of lycopene were revealed for the first time, to the best of our knowledge.
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    Changes on the Pancreas in Experimental Diabetes and the Effect of Lycopene on These Changes: Pdx-1, Ngn-3, and Nestin Expressions
    (Wiley, 2017) Sandikci, Mustafa; Karagenc, Levent; Yıldız, Mustafa
    The aim of the present study was to investigate changes occurring in the number of beta cells, as well as the expressions of Ngn-3, nestin and Pdx-1 of pancreatic progenitor cells in the pancreas of experimentally-induced adult diabetic rats and to determine the effect of orally-administered lycopene on these changes. Following the administration of 50 mg/kg streptozotocin to rats, four groups of animals were established: control+corn oil, control+lycopene, diabetic+corn oil and diabetic+lycopene. The animals in the control+lycopene and diabetic+lycopene groups received 4 mg/kg lycopene for a period of four weeks. The expressions of insulin, Ngn-3, nestin, and Pdx-1 were determined through immunohistochemistry in sections taken from pancreas tissue samples at the end of the experiment. The number of insulin-positive cells was found to be significantly low in the diabetic groups compared to the control groups. In addition, the presence of Ngn-3 and nestin-positive cells within the exocrine pancreas surrounding the islands was noted in the diabetic groups. Lycopene, in general did not have any effect in any of the parameters analyzed in the present study. It is suggested that these cells would function as stem cells to replace the lost beta-cell population. It is also suggested that it is possible to demonstrate the antioxidant effects of lycopene in the pancreas of diabetic rats by increasing the dose and duration of lycopene administration. Anat Rec, 300:2200-2207, 2017. (c) 2017 Wiley Periodicals, Inc.
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    The effects of Vitamin E on periodic acid Schiff reaction, qfantity of argyrophilic Nfcleolar organizer regions and indfcible nitric oxide synthase expression in prostate of rats exposed to bisphenol A
    (Parlar Scientific Publications, 2019) Yıldız, Mustafa; Gules, Ozay; Sandikci, Mustafa; Kum, Sadiye
    The aim of the study is to determine the protective role of Vitamin E on the prostate of rats exposed to bisphenol A (BPA). A total of 40 adult male Wistar Albino rats were used. The rats were randomly divided into five groups as follows: control, sham, Vitamin E, BPA, and Vitamin E+BPA. The applications of Vitamin E (300 IU/kg/day) and BPA (10 mg/kg/day) were proceeded for three weeks. Periodic Acid Schiff (PAS) and Silver-Staining for Nucleolar Organizer Region (AgNOR) methods were applied to prostate sections for histochemical examinations. Also, inducible nitric oxide synthase (iNOS) expression was examined immunohistochemically. The number of AgNOR positive dots and intensity of iNOS expression in glandular epithelial cells of prostate increased in BPA group compared to control group. Also, iNOS expression decreased in Vitamin E+BPA group compared to BPA group. On the other hand, a significant difference wasn’t found between the groups in terms of PAS reaction. As a conclusion, our findings demonstrate that BPA might stimulate cell proliferation and oxidative changes in prostate. The usage of Vitamin E might contribute to reducing oxidative damage induced by BPA in prostate. © by PSP
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    THE EFFECTS OF VITAMIN E ON PERIODIC ACID SCHIFF REACTION, QUANTITY OF ARGYROPHILIC NUCLEOLAR ORGANIZER REGIONS AND INDUCIBLE NITRIC OXIDE SYNTHASE EXPRESSION IN PROSTATE OF RATS EXPOSED TO BISPHENOL A
    (Parlar Scientific Publications (P S P), 2019) Yıldız, Mustafa; Gules, Ozay; Sandikci, Mustafa; Kum, Sadiye
    The aim of the study is to determine the protective role of vitamin E on the prostate of rats exposed to bisphenol A (BPA). A total of 40 adult male Wistar Albino rats were used. The rats were randomly divided into five groups as follows: control, sham, vitamin E, BPA, and vitamin E+BPA. The applications of vitamin E (300 IU/kg/day) and BPA (10 mg/kg/day) were proceeded for three weeks. Periodic Acid Schiff (PAS) and Silver-Staining for Nucleolar Organizer Region (AgNOR) methods were applied to prostate sections for histochemical examinations. Also, inducible nitric oxide synthase (iNOS) expression was examined immunohistochemically. The number of AgNOR positive dots and intensity of iNOS expression in glandular epithelial cells of prostate increased in BPA group compared to control group. Also, iNOS expression decreased in vitamin E+BPA group compared to BPA group. On the other hand, a significant difference wasn't found between the groups in terms of PAS reaction. As a conclusion, our findings demonstrate that BPA might stimulate cell proliferation and oxidative changes in prostate. The usage of vitamin E might contribute to reducing oxidative damage induced by BPA in prostate.