Yazar "Ozbey, Nilgun" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • [ X ]
    Öğe
    Epidemiological Evaluation of a Rapidly-Prevented Tularemia Outbreak in Canakkale Province, Turkey
    (Ankara Microbiology Soc, 2011) Otkun, Muserref Tatman; Alper Akçalı; Karadenizli, Aynur; Ozbey, Nilgun; Gazel, Deniz; Sener, Alper; Guclu, Oguz
    Tularemia is a disease caused by Francisella tularensis and widely seen at northern hemisphere of the world. In Turkey, oropharyngeal infections caused by a less virulent serotype F.tularensis subsp. holarctica are more prevalent. The aim of this study was to present the results of an epidemiological research performed after the detection of tularemia cases from Biga county of Canakkale province, Turkey, in December 2009. Following the report of two tularemia suspected cases from two villages (Baliklicesme and Sinekci) of Biga, an epidemiological investigation was undertaken to inspect the situation in this area. Water samples, clinical samples as throat swabs, wound swabs and serum samples were collected. Samples were cultured on heart agar supplemented with sheep blood, cysteine and antibiotics. Cultures were incubated at 37 degrees C in 5% CO(2) and followed for 10 days. Suspected colonies were identified by slide agglutination test using F.tularensis antisera. F.tularensis antibodies were investigated by standard tube agglutination method. Positive results obtained with agglutination test were also checked for a probable cross-reaction with Brucella antibodies by Rose-Bengal test. Water and wound samples were investigated using real-time polymerase chain reaction (RT Taqman PCR; Quantica, Techne Inc, UK) with probe and primers specific for ISFtu2 gene. All of the cultures yielded negative results, however eight of 16 water samples, one lymph node aspirate and one throat sample were found positive in F.tularensis TaqMan RT-PCR test. In tube agglutination test positive antibody titers between 1:20-1:1280 were detected in 36 of 115 serum samples. Two cases with antibody titers of 1:1280 and accompanying acute clinical findings, were diagnosed as tularemia and treated accordingly. Lymphatic drainage fluid samples obtained from one of these patients yielded positive result in PCR, however clinical sample could not be obtained from the other patient. The only epidemiological linkage between these acute cases (n= 2) and the other seropositive subjects (n= 34) was the use of local water supply system. It was learned that water obtained through reverse osmosis system had been used as drinking water at Baliklicesme village. Pre- and post-reverse osmosis system water samples from Baliklicesme village and samples from water supply of Sinekci village revealed positive results for F.tularensis by PCR. Since the only epidemiological relation between these two villages was using local water supply, tularemia cases encountered in this area were attributed to a water-borne epidemic and an automatic chlorination system was set up at each water reservoir in these villages. The establishment of these preventive measures curbed the growth of the epidemic. The cases presenting with throat sore, fever, lymphadenopathy (more than 2 cm), non-responsive to beta-lactam antibiotics, should be further investigated for tularemia. This work emphasizes that systematic setup and control of water disinfection systems are crucial to prevent tularemia outbreaks. Community and related authorities should be educated about the importance of water sanitation and chlorination.
  • [ X ]
    Öğe
    Evaluation of tuberculosis laboratory results in çanakkale onsekiz mart university research and education hospital for 2009-2011
    (2012) Ozbey, Nilgun; Alper Akçalı; Tatman-Otkun, Muserref
    Objective: Tuberculosis microbiological laboratory diagnosis was firstly started in year 2009, in Microbiology Laboratory of Onsekiz Mart University Research and Education Hospital in Çanakkale. We aimed at this study to present our laboratory data and to evaluate the methods which were used for the diagnosis of micobacteria. Method: Samples sent to our laboratory for tuberculosis culture were stained by Ehrlich-Ziehl-Neelsen (EZN) method and evaluated microscopically. After processing of samples, each sample was inoculated to Löwenstein-Jensen medium (LJ) and BACTEC MGIT 960 (Mycobacteria Growth Indicator Tube, Becton Dickinson, USA) liquid based medium. If suspected growth was detected, Mycobacterium tuberculosis complex (MTBC) typing was made and if requested antituberculosis drug susceptibility for streptomycin (STR), isoniazid (INH), rifampicin (RF) and ethambutol (ETM) tested. Samples from normally sterile body sites cultured directly, others were firstly decontaminated and concentrated. Results: During the study period 1.048 samples from 667 patient has been processed. Seventy eight samples (7.44%) from 54 patients were found positive by BACTEC MGIT system: 71 of them MTBC and seven of them were mycobacteria other than tuberculosis (MOTT). By LJ medium 64 MTBC and 4 MOTT strain, totally 68 mycobacterium were isolated. Mean time for detecting positive culture by MGIT 960 was 11.8 days (± 7.45 SD). With EZN stain, 49 samples were detected as acido resistant bacilli and only 42 (86%) of them were positive by culture. Antituberculosis drug susceptibilty was evaluated at isolates of 25 from 54 patients. A resistance to at least one of the drugs were detected in six isolates. It is found that five isolates were resistant to STR, three were resistant to INH and one was resistant to ETM. Three isolates were resistant to both STR and INH. Rifampicin resistance was not detected in MTBC. Conclusion: With this study we presented first tuberculosis laboratory findings from our province, Çanakkale. Tuberculosis microbiological culture and antituberculosis susceptibility tests can be made using Bactec MGIT 960 system which is easier and faster than solid media culture. In tuberculosis diagnosis sensitivity of culture is higher than microscopical evaluation. It was concluded that although microscopic examination has low sensitivity, for early detection of tuberculosis both culture and staining should be used together for routine detection of tuberculosis.
  • [ X ]
    Öğe
    What Should be the Antibiotic Preference in the Treatment of Bacterial Conjunctivitis?
    (Galenos Publ House, 2010) Eser, Ilker; Alcali, Alper; Comez, Arzu Taskuran; Komur, Bans; Ozbey, Nilgun; Otkun, Muserref Tatman
    Purpose: To investigate the pathogens associated with bacterial conjunctivitis and the in vitro antibiotic sensitivities of these bacteria. Material and Method: Forty-seven (27 female, 20 male) patients with a mean age of 43.7 26.4 (range: 1-84) years, who referred to our out-patient clinic with complaints of burning sensation, stringy discharge and hyperemia between December 2008 and March 2010, who were diagnosed with acute bacterial conjunctivitis, and had no history of any systemic or topical antibiotic use were induded in the study. Samples were taken from both eyes using cotton swabs, cultured onto chocolate and blood agar, and prepared for Gram staining. The identification of organisms was performed by Vitek2 compact system (bioMerieux, France). Antibiograms were evaluated according to the Clinical and Laboratory Standards Institute (CLSI) criteria by disc diffusion method. Results: Twenty-nine of 47 (61.7%) samples were culture-positive. The most common isolated bacteria were coagulasenegative staphylococci (16 cases, 55%). For them, the most sensitive antibiotics, given in decreasing order, were as follows: vancomycin (100%, 21/21), netilmicin (95.7%, 22/23), chloramphenicol (92.6%, 25/27) and tobramycin (91.3%, 21/23). Fluoroquinolones were relatively less sensitive: ofloxacin (75%, 21/28), moxifloxadn (75%, 18/24), dprofloxacin (73.1 %, 19/26). Discussion: Netilmidn, chloramphenicol and tobramydn were found to be more sensitive compared to fluoroquinolones in the treatment of bacterial conjunctivitis. Using these antibiotics as an empirical treatment, taking conjunctival culture of particular cases prior to treatment, and antibiotic switching according to antibiogram will be the most reasonable approach in case of no response to treatment.