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Öğe 99mTc Radionuclide-labeled and hydrogel-coated BaTiO3 nanocomposites(Elsevier, 2024) Ekici, Sema; Ozdemir, Semra; Puren, Busra AydurThe current developments such as improving of radionuclides and drug carriers in nuclear medicine has been utilized for cancer diagnosis, prevention and treatment, in other words in theranostics. In radionuclide-based imaging, radionuclides can be conjugated to the nanoparticle and directed to the targeted tissue. Inorganic nanoparticles are capable of imaging and treatment of tumors and have demonstrated unique physicochemical and biological properties. In present study, m-BaTiO3 nanocomposites were prepared by coating biocompatible and nanosized BaTiO3 particles (tra-BaTiO3) with poly (3-acrylamidopropyl) trimethyl-ammonium chloride (pAPTMACl) ionic hydrogel. The prepared m-BaTiO3 nanocomposites were then modified with (TcO4-)-Tc-99m selected as radionuclide and 8-hydroxy-7-iodo-5-quinoline sulfonic acid (SQ) which is a drug used in the treatment of cancer, to form m-BaTiO3-(TcO4-)-Tc-99m and m-BaTiO3-SQ radiopharmaceutical nanocomposites, respectively. The characterizations of prepared nanoparticles were carried out with ATR-linked FT-IR and Raman spectroscopy, DLS, zeta potential, TEM, SEM, SEM-Element Mapping analyzes. Radiolabeling studies of m-BaTiO3 nano- composites were monitored with a radionuclide dose calibrator. The suspension stability of tra-BaTiO3 nano-particles was increased after the conjugation of (TcO4-)-Tc-99m and SQ molecules. Particle size values ranking found as; m-BaTiO3-(TcO4-)-Tc-99m < m-BaTiO3-SQ < m-BaTiO3 < tra-BaTiO3. Zeta potential values were measured as -9.80 +/- 1.12 mV,-7.54 +/- 4.46 mV, -21.30 +/- 0.32 mV, -25.60 +/- 0.35 mV for tra-BaTiO3 , m-BaTiO3 , m-BaTiO3-(TcO4-)-Tc-99m, m-BaTiO3-SQ, respectively. The amount of (99m) TcO4- attached to the tra-BaTiO3 nanoparticles increased threefold, after coating with the hydrogel. It was observed that 76 % of SQ drug molecules loaded onto m-BaTiO3-SQ nanocomposites was released within 10 h. Herein, we highlighted the design of BaTiO3 nano-particles with biocompatible and flexible pAPTMACl hydrogel, (99m) Tc radionuclide, and SQ cancer drug for using of radiopharmaceuticals in cancer imaging and radiotherapy in order to be a pioneer study for our researches in the future.Öğe Colloidal drug carries from (sub)micron hyaluronic acid hydrogel particles with tunable properties for biomedical applications(Elsevier Sci Ltd, 2010) Ilgin, Pinar; Avci, Gulden; Sılan, Coşkun; Ekici, Sema; Aktas, Nahit; Ayyala, Ramesh S.; John, Vijay T.Hyaluronic acid (HA) hydrogel particles were synthesized in a single step employing water-in-oil microemulsion system. The HA particles were formed in the micro-environments of aqueous HA solution in oil by chemical crosslinking with divinyl sulfone (DVS). To produce magnetic field responsive HA-composite particles, iron magnetic nanoparticles were introduced into microemulsion system during synthesis to obtain HA-magnetic composites. For this purpose, iron nanoparticles were separately synthesized and mixed with linear HA followed by chemical crosslinking of linear HA with DVS in the micro-environments to envelope magnetic metal nanoparticles in the emulsion system. Scanning electron microscopy (SEM), dynamic light scattering (DLS) studies, and zeta potentials measurement were performed for particle size, charge and morphological characterization. Additionally. HA particles were chemically modified to induce desired functional groups on the particle surface and utilized for potential drug delivery vehicles. Trimethoprim (TMP) a bacteriostatic antibiotic drug were used as a model drug for the release studies in phosphate buffer solution (PBS) at pH 7.4 from bare HA, magnetic HA-composite and modified HA hydrogel particles. (C) 2010 Elsevier Ltd. All rights reserved.Öğe Development of polyampholyte hydrogels based on laponite for electrically stimulated drug release(Wiley, 2015) Ekici, Sema; Tetik, AycaPolyampholyte hydrogels (PAHs) composed of laponite, polyacrylamide and poly(3-acrylamidopropyl)trimethylammonium chloride crosslinked with ethylene glycol dimethacrylate were synthesized and characterized for their sensitivity to external conditions and their ability to control the release of the active drug agent paracetamol. Three PAHs were synthesized by taking the weight ratio of laponite to total monomer as 7, 15 and 29%. PAHs were characterized using Fourier transform infrared spectroscopy, thermogravimetric analysis and swelling measurements carried out in various media. Equilibrium and pulsatile swelling studies were performed on all hydrogels to determine to what extent the hydrogels would respond to changes in environmental pH, ionic strength and electric field, and how fast that response would be. Paracetamol was loaded into the hydrogels, and was released into buffered solutions as a function of pulsatile changes in pH and electric field. (c) 2014 Society of Chemical IndustryÖğe Heterogeneous and homogeneous structure dextran-poly(methacrylic acid) interpenetrating network hydrogels: synthesis and an application study(Wiley, 2012) Ekici, SemapH-sensitive dextran-poly(methacrylic acid) (Dext-pMeAc) full interpenetrating network hydrogels (INHs) were prepared by simultaneous radical polymerization of methacrylic acid monomer (MeAc) and Dext polymer chains in the presence of N,N-methylenebisacrylamide (MBA) as crosslinker in aqueous solution. These hydrogels were investigated as a drug carrier. The influence of MeAc and MBA contents in the network hydrogels on the swelling behaviour and mechanical strength of prepared Dext-pMeAc INHs was evaluated. Dext-pMeAc INHs were characterized by Fourier transform IR spectroscopy, and kinetic swelling measurements were carried out in deionized water and in simulated gastric fluids (pH 1.1 and pH 7.4). Dext-pMeAc/1-1, Dext-pMeAc/3-1 and Dext-pMeAc/5-1 hydrogels with molar ratios of n(Dext)/n(MeAc) = 10 and n(MBA)/n(Dext) = 10, 30 and 50 respectively showed a core-shell structure when they swelled. This phenomenon was not observed in Dext-pMeAc/5-2, Dext-pMeAc/5-3 and Dext-pMeAc/5-5 hydrogels containing a higher amount of Dext in the gels. The swelling data proved the formation of INHs with pH-sensitive behaviour. A drug release study was performed using Rhodamine 6G fluorescent dye as a model hydrophilic bioactive molecule. The in vitro release rate of Rhodamine 6G from Dext-pMeAc/5-3 hydrogel was dependent on the pH of the release medium. (C) 2012 Society of Chemical IndustryÖğe Hyaluronic acid hydrogel particles with tunable charges as potential drug delivery devices(Elsevier Sci Ltd, 2011) Ekici, Sema; Ilgın, Pınar; Bütün, Sultan; Şahiner, NurettinWe report the synthesis of hyaluronic acid (HA) particles with positive and negative charges on their surfaces. HA-based particles were prepared using an aqueous solution of linear HA in a sodium bis(2-ethylhexyl) sulfosuccinate (AOT)-isooctane microemulsion system. The prepared HA particles were post modified, i.e., oxidized to aldehyde (HA-O) by NaIO4 treatment and then these HA-O particles were reacted with cysteamine (CYs) to obtain thiol groups on the surface of the HA particles. The thiolated HA particles (HA-CYs) were further exposed to radical polymerization with an anionic monomer, 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), and with a cationic monomer, 3-acrylamidopropyl-trimethylammonium chloride (APTMACl), to generate HA-based ionic hydrogel particles, HA-CYs-AMPS and HA-CYs-APTMACl, respectively. The prepared HA-based anionic and cationic particles illustrated strong pH dependent size variations. We demonstrated that HA-CYs-AMPS and HA-CYs-APTMACl particles can be used as drug delivery vehicles. Trimethoprim (TMP) and naproxen (NN) were used as model drugs in the drug delivery experiments. (C) 2011 Elsevier Ltd. All rights reserved.Öğe Intelligent poly(N-isopropylacrylamide)-carboxymethyl cellulose full interpenetrating polymeric networks for protein adsorption studies(Springer, 2011) Ekici, SemaPoly(N-isopropyl acrylamide) (PNIPAm)-carboxymethyl cellulose (CMC) full interpenetrating polymeric networks (IPNs), based on PNIPAm and CMC, were prepared and investigated for adsorption of biomolecules utilizing a model protein, bovine serum albumin (BSA). N-isopropyl acrylamide monomers were polymerized in the presence of a natural polymer, e.g., carboxymethyl cellulose sodium salt. N,N'-methylenebisacrylamide (CL) was used to crosslink PNIPAm and CMC chains and IPN formed simultaneously. Spectroscopic and thermal characterization of the hydrogels were done with IR spectroscopy and thermogravimetric analysis. The swelling properties of PNIPAm and PNIPAm-CMC hydrogels were investigated as functions of the medium pH, temperature, ionic strength, and BSA. It was observed that the adsorption of protein molecules onto the hydrogels was mainly dependent on temperature and pH of the environment during the experiments. The maximum adsorption capacity (X) was observed at pH 4.7 which is the isoelectric point of BSA and at 40 A degrees C for both hydrogels; and introducing CMC to PNIPAm increased the protein adsorption of the hydrogel. Adsorbed amounts of BSA were 26.70 mg g(-1) (4 A degrees C) and 38.70 mg g(-1) (40 A degrees C) for PNIPAm-CMC full IPN hydrogels. Adsorbed BSA (up to 80%) was eluted in the elution medium containing 0.1 mol dm(-3) NaSCN at pH 8.0. Synthesized cylindrically shaped PNIPAm-CMC full IPN hydrogels can be used for adsorption studies related to the removal of proteins in pH- and temperature-sensitive biotechnological areas.Öğe Interpenetrating polymeric network hydrogels for potential gastrointestinal drug release(John Wiley & Sons Ltd, 2007) Ekici, Sema; Saraydin, DursunNew interpenetrating polymeric network (IPN) hydrogels based on chitosan (C), poly(N-vinyl pyrrolidone) (PVP) and poly(acrylic acid) (PAAc), crosslinked with glutaraldchyde (G) and N,N'-methylenebisacrylamide (MBA), were prepared and investigated for potential gastrointestinal drug delivery vehicles utilizing a model drug, amoxicillin. IPN hydrogels were synthesized by simultaneous polymerization/crosslinking of acrylic acid monomer in the presence of another polymer (C) and crosslinker (G, MBA). Three different concentrations of glutaraldehyde were used (0.5, 1.0 and 2.0 w/w) to control the overall porosity of the hydrogels, named C-P-AAc/0.5, C-P-AAc/1.0 and C-P-AAc/2.0, respectively. Spectroscopic and thermal analyses such as Fourier transform infrared spectroscopy, thermogravimetric analysis and thermornechanical analysis were performed for IPN characterization. Equilibrium swelling studies were conducted for pH and temperature response behavior. Swelling studies were also carried out in simulated gastric fluid of pH = 1.1 and simulated intestinal fluid of pH = 7.4 to investigate possible site-specific drug delivery. It was found that the release behavior of the drug from these IPN hydrogels was dependent on the pH of the medium and the proportion of crosslinker in the IPN. It was observed that amoxicillin release at pH = 7.4 was higher than at pH = 1.1. The analysis of the drug release showed that amoxicillin was released from these hydrogels through a non-Fickian diffusion mechanism. (c) 2007 Society of Chemical Industry.Öğe Investigation of the effect of laponite and crosslinkers on thermal, mechanical, swelling and conductive properties of the phtagel composite films(Iop Publishing Ltd, 2017) Ekici, Sema; Gungor, GizemPhytagel (PHG)-laponite (L) composites were prepared in the form of films by using PHG known to be biocompatible with the body, L clay which has electrical conductivity, and weak and strong crosslinkers, i.e., 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (E) and Cu2+, respectively. Composites were obtained by using laponite in two different ratios and only one concentration of Cu2+. Bare PHG and PHG films crosslinked with E were also prepared simultaneously and characterization of all the films are presented comparatively. Cu2+ ions are more effective crosslinkers than E molecules for PHG chains and increased the mechanical strength of composites as revealed in swelling studies and differential scanning calorimetry analysis. It was determined that laponite and Cu2+ ions increased the conductivity of PHG hydrogel and conductivity decreased when the amount of laponite is increased. Wet film samples had conductivity between 0.95 x 10(-8) and 5.94 x 10(-8) S cm(-1) values, while dry films were not conductive. Wet PHG, PHG-E, PHG-E-L1, and PHG-E-L2 films showed bending deformation towards the anode under electric field of 25 V in magnitude, while wet PHG-Cu-L2 films tended to bend towards the cathode side. It was concluded that these composites are intelligent hydrogel composites which can respond to stimuli such as environmental pH and electric field changes and can be considered for use in studies involving low electrical conductivity.Öğe Kitosan temelli hidrojellerin hazırlanması ve 5-Florourasil salımı davranışlarının incelenmesi(2016) Ekici, SemaKitosan (CH), poli(2-akrilamido-2-metilpropansülfonik asit) (pAMPS) ve poliakrilamid (pAAm) hidrojelleri içeren, üçlü, iç içe geçmiş ağ yapılı hidrojeller (IPNHs) ve sadece kitosan hidrojelleri sentezlendi ve model ilaç olarak seçilen 5-Florourasil (5-FLU) biyoetken moleküllerinin denetimli salımı için araştırıldı. IPNHs; çapraz bağlayıcı olarak seçilen glutaraldehit (GLU) ve N,N'-metilenbisakrilamid (MBA) varlığında monomerlerin radikalik polimerleşmesi ile hazırlandı. Hidrojellerin spektroskopik analizleri; Fourier dönüşümlü infrared spektroskopisi ile yapıldı. IPNH'lerin kinetik ve dinamik şişme çalışmaları 37 oC'de; mide sıvısına (pH=1.1) ve ince bağırsak sıvısına (pH=7.4) benzetilmiş tampon çözeltilerde gastrointestinal ilaç salımı çalışmaları için in vitro olarak araştırıldı. Şişme ve ilaç salım deneysel verileri; şişme ve salım proseslerinin ikinci dereceden kinetiğe uyduğunu gösterdi. Tutuklanan biyoetken türlerin IPNH'lerden ve kitosan hidrojellerinden salımının; hidrojel bileşimi ve ortamın pH'sına bağlı olduğu anlaşıldı. Sonuç olarak; kitosan temelli IPNH'lerin; oral gastrointestinal salım sistemlerindeki formülasyonlar için ümit verici adaylar olabileceği söylenebilirÖğe P(4-vinyl pyridine) hydrogel use for the removal of UO22+ and Th4+ from aqueous environments(Academic Press Ltd- Elsevier Science Ltd, 2011) Özay, Özgür; Ekici, Sema; Aktaş, Nahit; Şahiner, Nurettin4-vinyl pyridine (4-VP) based hydrogels with 2-hydroxyethylmetacrylate (HEMA) and magnetic composites were prepared and tested for use in the removal of UO22+. and Th4+ ions from aqueous environments. It was found that the absorption of these metal ions from aqueous environments decreased with an increase in the amount of HEMA contained within p(4-VP-co-HEMA) hydrogels between 0.498 mmol for pure p(4-VP) and 0.027 mmol for pure p(HEMA). The characterization of the hydrogels was determined by swelling experiments, FT-IR and thermal analysis. The effects of initial metal ion concentration, hydrogel amount and the temperature of the medium on absorption of the ions were investigated. Langmuir and Freundlich isotherms were constructed for the absorption of UO22+ and Th4+. Both isotherms demonstrated that these metal ions complied with monolayer absorption kinetics. (C) 2011 Elsevier Ltd. All rights reserved.Öğe PMSE 489-Poly(4-vinylpyridine) based nanoparticle syntheses and their applications to functional materials(Amer Chemical Soc, 2008) Özay, Özgür; Aydin, Fatma; Ekici, Sema; Yıldız, Mustafa; Baran, Yakup; Tan, Grace; John, Vijay T.[Anstract Not Available]Öğe Poly(acrylamide-sepiolite) composite hydrogels: Preparation, swelling and dye adsorption properties(Springer, 2006) Ekici, Sema; Isikver, Yasemin; Saraydin, DursunPolymer-clay hydrogel composite was prepared on the basis of polyacrylamide (PAAm) gel containing the clay mineral sepiolite. The properties of swelling and dye adsorption of poly(acrylamide-sepiolite) (AAm/Sep) composite hydrogel were investigated. The parameters of swelling and diffusion in water and dye solutions were calculated for the AAm and AAm/Sep hydrogels. It was found that the equilibrium swelling degree of obtained composite higher than that of AAm gel. Spectroscopic analysis of composite and composite-dye systems was done with FT-IR method. Adsorption of monovalent cationic dyes such as Basic Blue 12 (BB-12) Basic Blue 9 (BB-9), and Basic Violet 1 (BV-1), was studied on the composite. In the adsorption experiments, S (Sigmoidal) type for composite gel adsorption isotherms in the Giles classification system was found. Adsorption studies indicated that the amounts of adsorbed dyes on the AAm/Sep composite hydrogel were increased with following order; BB-12 > BB-9 > BV-1. The composite hydrogel may be considered as good candidate for environmental application to retain more water and dyes.Öğe Polyacrylamide-polyaniline composites: the effect of crosslinking on thermal, swelling, porosity, crystallinity, and conductivity properties(Springer, 2019) Celik, Mehmet Umur; Ekici, SemaWhen the synthesis of hydrogels, which are a hydrophilic group of polymers, occurs below the freezing point of water, the hydrogels gain a perfect property: porosity. Hydrogels with this property and resulting sponge-like structure are called cryogels (CRYs). The porosity of cryogels is affected by the amount of crosslinker used. In this study, how the amount of crosslinker affected the porosity initially was investigated, along with thermal, swelling, and crystallinity properties of polyacrylamide (PAAM) cryogels. Additionally, a series of composites (COMs) were synthesized with polyaniline (PAN) in the pore spaces of CRYs and again the effect of varying pore size on the electrical conductivity of COMs was researched. In the cryogel series, as the amount of N,N '-methylenbisacrylamide (MBA) increased, the pore size increased. The S, P, P-s, and V-p parameters increased in general with the MBA increase. The conductivity values of composites were determined in the interval of 1.6 x 10(-3)-4.5 x 10(-3) S cm(-1).Öğe Removal of toxic metal ions with magnetic hydrogels(Pergamon-Elsevier Science Ltd, 2009) Özay, Özgür; Ekici, Sema; Baran, Yakup; Aktaş, Nahit; Şahiner, NurettinHydrogels, based on 2-acrylamido-2-methyl-1-propansulfonic acid (AMPS) were synthesized via photopolymerization technique and used for the preparation of magnetic responsive composite hydrogels. These composite hydrogels with magnetic properties were further utilized for the removal of toxic metal ions such as Cd(II), Co(II), Fe(II), Pb(II), Ni(II), Cu(II) and Cr(III) from aqueous environments. It was revealed that hydrogel networks with magnetic properties can effectively be utilized in the removal of pollutants. The results verified that magnetic iron particle containing p(AMPS) hydrogel networks provide advantageous over conventional techniques. Langmuir and Freundlich adsorption isotherms were applied for toxic metal removal and both isotherms were fit reasonably well for the metal ion absorptions. (c) 2009 Elsevier Ltd. All rights reserved.Öğe Temperature and magnetic field responsive hyaluronic acid particles with tunable physical and chemical properties(Elsevier, 2011) Ekici, Sema; Ilgın, Pınar; Yılmaz, Selahattin; Aktaş, Nahit; Şahiner, NurettinWe report the preparation and characterization of thiolated-temperature-responsive hyaluronic acid-cysteamine-N-isopropyl acrylamide (HA-CYs-NIPAm) particles and thiolated-magnetic-responsive hyaluronic acid (HA-Fe-CYs) particles. Linear hyaluronic acid (HA) crosslinked with divinyl sulfone as HA particles was prepared using a water-in-oil micro emulsion system which were then oxidized HA-O with NaIO4 to develop aldehyde groups on the particle surface. HA-O hydrogel particles were then reacted with cysteamine (CYs) which interacted with aldehydes on the HA surface to form HA particles with cysteamine (HA-CYs) functionality on the surface. HA-CYs particles were further exposed to radical polymerization with NIPAm to obtain temperature responsive HA-CYs-NIPAm hydrogel particles. To acquire magnetic field responsive HA composites, magnetic iron particles were included in HA to form HA-Fe during HA particle preparation. HA-Fe hydrogel particles were also chemically modified. The prepared HA-CYs-NIPAm demonstrated temperature dependent size variations and phase transition temperature. HA-CYs-NIPAm and HA-Fe-CYs particles can be used as drug delivery vehicles. Sulfamethoxazole (SMZ), an antibacterial drug, was used as a model drug for temperature-induced release studies from these particles. (C) 2010 Elsevier B.V. All rights reserved.Öğe The Removal of Textile Dyes with Cross-Linked Chitosan-Poly(acrylamide) Adsorbent Hydrogels(Taylor & Francis Inc, 2011) Ekici, Sema; Guntekin, Gamze; Saraydin, DursunThis study examined the behaviors and mechanisms of chitosan (CS)-poly(acrylamide) (PAAM) full interpenetrating polymeric network (IPN) hydrogels as an adsorbent to remove EY-4GL and S-Blue textile dyes from an aqueous solution. CS-PAAM IPN hydrogels were prepared by acrylamide monomer polymerization in the presence of a natural polymer, e. g., chitosan. N,N'-methylenebisacrylamide (MBAM) and glutaraldehyde (GLA) were selected to cross-link PAAM and CS chains and a full-IPN structure formed simultaneously. Kinetic swelling studies of CS-PAAM IPNs were carried out with deionized water and aqueous dye solutions. The experimental data clearly suggested that the swelling process obeys second-order kinetics. Network and diffusion parameters for CS-PAAM and PAAM hydrogels were calculated and it was observed that these IPN hydrogels have high cross-linking efficiencies in comparison to PAAM hydrogels. Adsorption of textile dyes onto hydrogels was studied by a batch adsorption technique at 23 degrees C and 40 degrees C, and it was seen that the higher temperature increased the dye adsorption onto the hydrogels. L type (Lan gmuir) adsorption isotherms, according to Giles classification system, were established at the end of adsorption experiments. The prepared IPN hydrogels show good ability to uptake textile dyes from wastewater.Öğe Utilization of magnetic hydrogels in the separation of toxic metal ions from aqueous environments(Elsevier, 2010) Özay, Özgür; Ekici, Sema; Baran, Yakup; Kubilay, Şenol; Aktaş, Nahit; Şahiner, NurettinWe report the synthesis of poly(2-acrylamido-2-methyl-1-propansulfonic acid-co-vinylimidazole), p(AMPS-c-VI) hydrogels from various amounts of 2-acrylamido-2-methyl-1-propansulfonic acid (AMPS) and N-vinylimidazole (VI) monomers by photo-polymerization technique. Hydrogel composites with magnetic properties were synthesized in situ by incorporating Fe(II) and Fe(III) ions into p(AMPS-c-VI) hydrogels network and then reducing them with alkaline solution. Hydrogels swellings were performed for both bare and magnetic hydrogels. The selective removal capability of toxic metal ions, Cu(II), Cd(II), Fe(II), and Pb(II) by these hydrogels was studied in aqueous medium. Desorption studies were also performed in weakly acidic media to observe whether these hydrogel composites can be utilized as reusable tool for the toxic metal ion removal. The effects of the gel amount, contact time with metal ions, metal species and metal ion concentrations on absorption phenomena were evaluated. The equilibrium removal process of metal ions by the composite hydrogels complies well with the Langmuir adsorption isotherm model. (C) 2010 Elsevier B.V. All rights reserved.Öğe Utilization of polyampholyte hydrogels for simultaneous removal of textile dyes from aqueous solutions(Taylor & Francis Inc, 2018) Ekici, Sema; Guntekin, GamzeThe study aimed to create hydrogel structures with greater functionality, otherwise known as polyampholytes (PAHs), and to use these in simultaneous removal studies for remazol-type dyes found in environmental waste waters. Adsorption studies were accomplished at 20 degrees C and 35 degrees C, indicated that adsorption process was exothermic in nature. The Q(m) values for the PAH-dye systems were in the interval 111-122mg dye/g PAH with Ads% from 94% to 98%. The ability of PAH hydrogels to adsorb two different species of dye molecules at the same time is an advantage of PAH adsorbents compared to other hydrogel adsorbents prepared in this area.
