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    Öğe
    Determination of isozyme polymorphism in open-pollinated olive (Olea europaea L.) seedlings used as rootstock
    (International Society Horticultural Science, 2008) Seker, M.; Dulger, S.; Kaynas, N.; Yucel, Z.
    Some important olive cultivars like 'Domat' and 'Tavsan Yuregi' are commercially grafted on seedlings which are obtained from open-pollinated seeds of 'Uslu' olive cultivar, which usually produces morphologically uniform populations. In this study, seedling populations of 'Uslu' cultivar were evaluated for their isozyme genotypes. Starch gel electrophoresis for six isozyme systems including malate dehydrogenase (MDH), alcohol deshydrogenase (ADH), isocitrate deshydrogenase (IDH), phosphogluco mutase (PGM), phosphogluco isomerase (PGI) and peroxsidase (PRX) were investigated. Total loci and number of alleles were determined in 50 seedlings. Total 18 alleles were determined at 7 isoenzymatic loci. The seedlings were classified into 9 groups according to their isozyme profiles. ADH and MDH systems were monomorphic among the seedlings. However, PGI and IDH systems were polymorphic due to high level of heterozygosities. PGM and PRX systems exhibited only two isozyme genotypes among the seedlings. This study demonstrates that clonal rootstocks need to be used instead of seedlings for olive cultivars, which have to be grafted for propagation.
  • [ X ]
    Öğe
    Investigation of genetic diversity in olive (Olea europaea L.) cultivars and types using isozyme analysis
    (Int Soc Horticultural Science, 2008) Seker, M.; Dulger, S.; Kaynas, N.
    Olive (Olea europaea L.) shoot tip extracts were analyzed by starch gel electrophoresis for isozymes of Malate dehydrogenase (MDH), Alcohol deshydrogenase (ADH), Isocitrate deshydrogenase (IDH), Phosphogluco mutase (PGM), Phosphogluco isomerase (PGI) and Peroxidase (PRX). Total loci, number of alleles and genetical distances were determined in 43 olive genotypes (29 local and introduced cultivars, 5 'Gemlik' types, 3 'Ayvalik types, 1 'Memecik' type and 5 olive seedlings of 'Uslu' cultivar). High level isoenzymatic variation was detected in olive genotypes and total 23 alleles were determined at 7 loci which belong to the isozyme systems evaluated. The genotypes divided into 7 groups for MDH, 2 groups for ADH, 7 groups for IDH, 5 groups for PGM, 7 groups for PGI and 3 groups for PRX profiles. The genetic distance was the highest between 'Egriburun' and 'Manzanilia' while it was the lowest between 'Tavsan Yuregi' and 'Memecik' cultivars.