Yazar "Cobanoglu, Hayal" seçeneğine göre listele
Listeleniyor 1 - 9 / 9
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Assessment of the genotoxic potential of a migraine-specific drug by comet and cytokinesis-block micronucleus assays(Taylor & Francis Ltd, 2020) Cayir, Akin; Cobanoglu, Hayal; Coskun, MunevverBackground: Eletriptan is a migraine-specific drug-containing the triptan group. In terms of drug safety, the present study aimed to investigate the genotoxic potential of eletriptan. Research design & methods: We conducted our study by using the cytokinesis-block micronucleus cytome (CBMN) assay, a comprehensive method for measuring micronucleus formation, and a sensitive method for detecting DNA-strand breaks. In the assay, cytokinesis-block proliferation index and the frequency of micronuclei were evaluated in lymphocytes treated with three different concentrations (1, 10 and 25 mu g/ml) of eletriptan for 48 hours. In comet assays, DNA damage was evaluated in leucocytes treated with three different concentrations (1, 10 and 25 mu g/ml) of eletriptan for an hour. Results: Eletriptan did not induce cytotoxicity nor any increased micronuclei frequencies. While the comet parameters % DNA in tail, tail moment, and the olive moment was found to be significantly increased at 10 and 25 mu g/ml, the cytokinesis-block proliferation index values were not. Conclusion: These findings suggest that eletriptan is non-cytotoxic but potentially weakly genotoxic at higher concentrations (10 and 25 mu g/ml).Öğe Comet assay for assessment of DNA damage in greenhouse workers exposed to pesticides(Taylor & Francis Ltd, 2019) Cayir, Akin; Coskun, Mahmut; Coskun, Munevver; Cobanoglu, HayalPurpose: The main goal of the present study was to determine DNA damage in pesticide-exposed greenhouse workers and pesticides non-exposed controls. Materials and methods: The DNA damage was measured by alkaline comet assay method (pH > 13) in 41 greenhouse workers and 45 non-exposed individuals as the control. Pesticide exposure was assessed by duration of working in the greenhouse and pesticide application in the greenhouse time. DNA damage was estimated by arbitrary unit and damage frequency. Results: Arbitrary unit and damage frequency were consistently significantly higher in greenhouse workers than those of the controls (p = 0.001). In terms of gender in greenhouse, DNA damage of female workers was significantly higher than those in male workers (p < 0.05). We found significant correlation between DNA damage and working hours spent. Multiple linear regression analysis showed that working hours in the greenhouse as an indication of pesticide exposure were significantly associated with the DNA damage, which can be attributed to the genotoxic potential of the pesticide mixture. Conclusions: The comet assay is sensitive to detect the damage exposed to chronic effect of pesticides in greenhouse workers. Significant DNA damage was obtained for the exposed group, which was associated with the pesticide exposure.Öğe Comparison of buccal micronucleus cytome assay parameters in the pesticide-exposed greenhouse workers with the controls(Elsevier Ireland Ltd, 2017) Cayir, Akin; Coskun, Munevver; Cobanoglu, Hayal; Coskun, Mahmut[Anstract Not Available]Öğe Different working conditions shift the genetic damage levels of pesticide-exposed agriculture workers(Springer Heidelberg, 2020) Cobanoglu, Hayal; Coskun, Munevver; Coskun, Mahmut; Cayir, AkinIn the current study, we had two main purposes. Firstly, we aimed to compare genetic damages in the agricultural workers of two different types of environmental conditions including the greenhouse and open fields. Secondly, we aimed to compare genetic damages in the total agricultural workers as the exposed group (greenhouse and open field workers) (n = 114) and the non-exposed control group (n = 98) living in the same area in Canakkale, Turkey. For these purposes, we investigated the incidence of micronucleus (MN), nucleoplasmic bridges (NPBs), and nuclear buds (NBUDs) in peripheral blood lymphocytes. We observed that the frequencies of MN, NPB, and NBUD obtained for the greenhouse workers were statistically significantly higher than those obtained for the open field workers. When the results of the control group were compared with those of the total workers, there were statistically significant differences in terms of MN and NBUD frequencies. We found that age and MN were correlated at a significant level in both the agricultural workers and the control group. The MN frequency of the female workers was 1.5 times greater than that of the male workers, and it was a significant level in the agricultural workers.Öğe DNA damage and circulating cell free DNA in greenhouse workers exposed to pesticides(Wiley, 2018) Cayir, Akin; Coskun, Mahmut; Coskun, Munevver; Cobanoglu, HayalPesticides widely used in agriculture and other applications have been linked to cancer and other diseases through several potential mechanisms. The goals of this study were to assess DNA damage in lymphocytes by the cytokinesis-block micronucleus assay (CBMN), and to measure circulating cell free DNA (ccf-DNA) in the blood of pesticide-exposed greenhouse workers and matched controls living in the same area. CBMN was applied to peripheral blood lymphocyte samples taken at different times (spring and autumn) for each individual. We measured plasma ccf-DNA levels using a Qubit((R)) fluorometer. The results indicated that the MNL, BNMN, and NBUDs frequencies of pesticide-exposed individuals were significantly higher than non-exposed individuals. Apart from MNL, BNMN and CBPI, a season-related effect was found for the NPB and NBUD frequencies. With MNL and BNMN as the dependent variables, multiple regression analysis showed that age and gender affected MN formation. The ccf-DNA level in the pesticide-exposed group was significantly higher than the control group. There was no seasonal variation regarding the free DNA amount. Ccf-DNA in males was found to be higher than females. The MNL, BNMN, NPB, and CBPI did not correlate with the ccf-DNA amount. It can be concluded that pesticide exposure can modulate DNA integrity via different mechanisms. Also, elevated levels of ccf-DNA could be recommended as a biomarker of pesticide exposure. Environ. Mol. Mutagen. 59:161-169, 2018. (c) 2017 Wiley Periodicals, Inc.Öğe Evaluation of genotoxic potential of pemetrexed by using in vitro alkaline comet assay(Marmara Univ, Fac Pharmacy, 2017) Cobanoglu, Hayal; Coskun, Munevver; Coskun, Mahmut; Cayir, AkinPemetrexed is a chemotherapeutic drug, approved for the treatment of mesothelioma and non-small cell lung cancer and variety of neoplasm. Chemotherapeutic drugs don't target only cancer cells in treatment. During treatment these drugs encounter with normal cells as well. Therefore, in this in vitro study, it was aimed to investigate the whether genotoxic effect or not of Pemetrexed on normal leukocytes. In vitro alkaline comet assay was used in this study. Leukocytes from human peripheral blood samples from two volunteer donors were used in the study. In the present study, leukocytes were treated with four different concentrations (25, 50, 75, and 100 mu g/ml) of the drug for 1 hour. At concentration of 75 mu g/ml and 100 mu g/ml, there were statistically significant increases in DNA damage compared to the negative control (p<0.05, and p<0.01 respectively). The obtained result shows that Pemetrexed may have genotoxic potential on normal leucocytes.Öğe In vitro genotoxic and cytotoxic effects of doxepin and escitalopram on human peripheral lymphocytes(Taylor & Francis Ltd, 2018) Cobanoglu, Hayal; Coskun, Mahmut; Cayir, Akin; Coskun, MunevverAntidepressants are drugs used for the treatment of many psychiatric conditions including depression. There are findings suggesting that these drugs might have genotoxic, carcinogenic, and/or mutagenic effects. Therefore, the present in vitro study is intended to investigate potential genotoxic and cytotoxic effects of the antidepressants escitalopram (selective serotonin reuptake inhibitor) and doxepin (Tricyclic antidepressant) on human peripheral lymphocytes cytokinesis-block micronucleus (CBMN), sister chromatid exchange (SCE), and single cell gel electrophoresis (alkaline comet assay) were used for the purpose of the study. In the study, four different concentrations of both drugs (1, 2.5, 5, and 10 mu g/mL) were administered to human peripheral lymphocytes for 24h. The tested concentrations of both drugs were found to exhibit no cytotoxic and mitotic inhibitory effects. SCE increase caused by 5 and 10 mu g/mL of escitalopram was found statistically significant, while no statistically significant increase was observed in DNA damage and micronucleus (MN) formation. Moreover, the increase caused by doxepin in MN formation was not found statistically significant. Besides, 10 mu g/mL of doxepin was demonstrated to significantly increase arbitrary unit and SCE formation. These findings suggest that the investigated concentrations of escitalopram and doxepin were non-cytotoxic but potentially genotoxic at higher concentrations.Öğe Micronucleus, nucleoplasmic bridge and nuclear bud frequencies in patients with laryngeal carcinoma(Pacini Editore, 2020) Yazici, Ibrahim; Caglar, Ozge; Guclu, Oguz; Cobanoglu, Hayal; Coskun, Mahmut; Coskun, Munevver; Kilic, AytacThe aim of the study is to determine and compare micronucleus (MN), nucleoplasmic bridge (NPB) and nuclear bud (NBUD) frequencies in patients with laryngeal carcinoma and healthy controls. The study was conducted in the School of Medicine of Onsekiz Mart University. A total of 102 volunteers, 51 of whom had laryngeal carcinoma and 51 of whom were healthy control subjects, participated in this study. The Cytokinesis-Block Micronucleus Assay (CBMN) was applied to peripheral blood lymphocytes taken from patients and controls. We evaluated MN, NPBs and NBUDs frequencies in patients with laryngeal carcinoma and compared the results with those in the control group. The frequencies of MN, NPBs and NBUDs of patients with laryngeal carcinoma were found significantly higher than those in the control group (P = 0.01, P = 0.004, P = 0.01, respectively). MN, NPB and NBUD frequencies were also compared in the patients with and without pesticide exposure, and the means of all frequencies was higher in patients with pesticide exposure (P = 0.001, P = 0.02 respectively). The MN, NPBs and NBUDs frequencies of the patients with laryngeal cancer were significantly higher than those of the control group, and pesticide exposure might be a risk factor that increases genomic instability and risk of laryngeal cancer.Öğe Results of buccal micronucleus cytome assay in pesticide-exposed and non-exposed group(Springer Heidelberg, 2019) Cobanoglu, Hayal; Coskun, Munevver; Coskun, Mahmut; Cayir, AkinSince many different pesticides have been used occupationally, there have been inconsistent results regarding DNA damages among greenhouse workers. Thus, the aim of the study is to evaluate DNA damages, cell death, and chromosomal instability by using the buccal micronucleus cytome (BMcyt) assay in greenhouse workers and to compare those with a non-exposed group. The BMcyt assay was applied to the exfoliated buccal cell samples collected from 66 pesticide-exposed and 50 non-exposed individuals. We evaluated the frequency of micronucleus (MN), nuclear bud (NBUD), binucleated (BN) cells, and karyolitic (KL), pyknotic (PY), and karyorrhectic (KH) cells. The results showed that the MN, BN, PY, and KH frequencies of the pesticide-exposed group were significantly higher than those of the controls (P?0.05, P?0.05, P?0.01, and P?0.05, respectively). We observed that the MN, BN, PY, and KH frequencies in the autumn were statistically different compared with those in the control group (P=0.037 for MN, P=0.001 for BN, P=0.016 for PY, and P=0.033 for KH). The same comparison was done in the spring for the control, and there was a statistically significant difference for MN (P=0.046) and PY (P=0.014). We can conclude that pesticide exposure in greenhouse workers was one of the factors that altered DNA damages, cell death, and chromosomal instability in oral mucosa cells.
