Yazar "Bahceci, Dilek Senol" seçeneğine göre listele

Listeleniyor 1 - 4 / 4
  • [ X ]
    Öğe
    Biological Activity and Optical Sensor Properties of Green Synthesis Polymer
    (Wiley-V C H Verlag Gmbh, 2022) Bahceci, Dilek Senol; Demir, Neslihan; Kocaeren, Aysel Aydin
    In this study, 4-amino-3-hydroxy-1-naphthalene sulfonic acid (Monomer) was converted to its enzyme-catalyzed oxidative polymerization (Poly-enz) in dioxane solvent by horseradish peroxidase enzyme (HRP) in the presence of hydrogen peroxide as oxidant. For the analysis of the monomer and its synthesized Poly-enz, H-1-NMR, C-13-NMR, FT-IR, TGA, and SEM measurements were determined. In addition, the optical ion sensor properties of Poly-enz against metals such as Ag+, Cd2+, Co2+, Hg2+, Ni2+, Pb2+, Zn2+, Cu2+ were examined with UV-Vis spectrophotometer. It was observed that Cu metal showed sensory properties. DNA cleavage and DNA binding activities of monomer and Poly-enz were done using by agarose gel electrophoresis and UV-Vis methods, respectively. It was seen that the Poly-enz interacted with DNA electrostatically. This enzymatic polymer was found to cleave pBR322 plasmid DNA both hydrolytically and oxidatively as well. The antioxidant activities of the compounds were examined by three different methods (DPPH, ABTS, FRAP) and it was found that the Poly-enz showed high antioxidant activity compared to its monomer. Antimicrobial activities were studied against various bacterial and yeast cultures by the minimum inhibition concentration (MIC) method. It was observed that the antimicrobial activities of the enzymatic polymer in different microorganisms increased depending on the concentration.
  • [ X ]
    Öğe
    From Wastewater to Worth: Eco-Friendly Nanoparticle Synthesis Based on Microalgae Cultivated in Industrial Wastewater
    (Wiley-V C H Verlag Gmbh, 2025) Kayan, Iremsu; Bahceci, Dilek Senol; Gulec, Neslihan; Dalgic, Busra; Oz, Nilgun Ayman
    Silver nanoparticles (AgNPs) were synthesized in this study through the utilization of microalgae (MA). The bioactive compounds included in microalgae make them natural antibacterial and antifungal agents. However, cultivating microalgae typically necessitates specialized medium, hence elevating expenses. Consequently, to lower costs, this study utilized industrial wastewater, specifically cheese whey wastewater (CWW). Therefore, a form of wastewater recycling has been accomplished, and a sustainable production process has been implemented. Silver nanoparticles were synthesized using Nannochloropsis sp., a type of microalgae, by the green synthesis process. Silver nanoparticles (MA + AgNPs, CWW + MA + AgNPs) derived from microalgae cultivated in both pure and cheese whey media have been synthesized. Transmission electron microscopy (TEM) examination and Fourier transform infrared spectroscopy (FT-IR) measurement have been conducted to observe the production of silver nanoparticles. Their biological actions have been compared. The results indicate that silver nanoparticles derived from microalgae exhibit significant antibacterial activity (inhibition zones up to 17 mm and minimum inhibition concentration [MIC] values down to 31.25 mu g/mL). The characteristics of silver nanoparticles derived from microalgae that influence antioxidant activity and DNA damage have been effectively demonstrated. This study reveals the biotechnological potential of microalgae and the environmental advantages of utilizing wastewater. These in vitro findings show that microalgae-derived nanoparticles display wide-spectrum antimicrobial activity even at very low concentrations and when this is considered in terms of sustainable production process, the mentioned nanoparticles may be promising materials that can be used in future medical and industrial application.
  • [ X ]
    Öğe
    Investigation of Synthesis, Characterization, and Biological Activities of Water-Soluble Polymer with Horseradish Peroxidase Enzyme
    (Maik Nauka/Interperiodica/Springer, 2022) Bahceci, Dilek Senol; Demir, Neslihan
    7-Amino-4-hydroxy-2-naphthalene sulfonic acid (AHNSA) was converted into p(AHNSA-HRP) by enzyme-catalyzed oxidative polymerization in the presence of horseradish peroxidase enzyme using hydrogen peroxide in dioxane solvent. The structural analysis of AHNSA and its enzyme-catalyzed oxidative polymer, were taken by H-1 NMR, C-13 NMR, FTIR measurements. The optical features by UV-Vis spectrophotometry, the decomposition temperatures by TGA and the morphological properties by SEM were determined. For biological activities of AHNSA and synthesized enzymatic polymer, DNA cleavage and DNA binding activities were done by agarose gel electrophoresis and by UV-Vis spectrophotometer, respectively. It was observed that the polymer interacted with DNA electrostatically in its DNA binding activity. As a result of the agarose gel electrophoresis studies, it was seen that the polymer cleaved the DNA both hydrolytically and oxidatively. Antioxidant properties, 2,2-diphenyl-1-picryl-hydrazyl, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), and ferric reducing antioxidant power detection were studied by three different methods. Antimicrobial activities were investigated against various bacterial and yeast cultures using the minimum inhibition concentration method. It was observed that both antioxidant and antimicrobial properties of the new enzyme-catalyzed oxidative polymer were higher than that of AHNSA.
  • [ X ]
    Öğe
    Optical Sensor Applications of Carbazole Derivative Polymers Containing Different Aliphatic Groups Against Fe3+ Metal Ion
    (Maik Nauka/Interperiodica/Springer, 2022) Kocaeren, Aysel Aydin; Bahceci, Dilek Senol; Dogan, Fatih
    The carbazole derivatives containing aromatic rings with long alkyl groups such as C-6, C-8, C-10 and 3,4-bis(4-iodophenoxy) thiophene are synthesized. The products of carbazole derivatives and 3,4-bis(4-iodophenoxy) thiophene interaction were polymerized using FeCl3 oxidant. The structural characteristics of the synthesized compounds were obtained by FT-IR and NMR spectroscopy. UV measurements are performed to determine the optical changes of the copolymers in the presence of metal ions such as Ag+, Cd2+, Co2+, Cu2+, Fe2+, Fe3+, Hg2+, Li+, Ni2+, Pb2+, Zn2+. The selectivity of Fe3+ is investigated by recording of the absorbance changes of Fe3+ solutions prepared at different concentrations. The effect of pH is also investigated in optical sensor studies. According to UV measurements, the materials containing thiophene and carbazole units can be considered as candidates for sensor production due to their selectivity towards Fe3+ ions.