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    Half-Sandwich Ru(II) Complexes Bearing 2-(2¢-quinoly)benzimidazoles with Anticancer Activity
    (AMG Transcend Association, 2024) Erdem, Ahmet; Mutlu, Dogukan; Kilincarslan, Rafet; Dayan, Osman; Arslan, Sevki
    In this article, cytotoxic and apoptotic properties of previously synthesized and characterized four different half?sandwich ruthenium (II) complexes (C1-C4) with 2-(2¢-quinoly) benzimidazole frameworks (L1-L5) were described. These Ru (II) complexes (C1-C4) had strong cytotoxic activity towards the human glioblastoma (U373) cancer cell line with low toxicity to the non-cancerous human embryonic kidney (HEK293) cell line. Mechanistic studies revealed that all complexes caused apoptosis induction by activating caspases with upregulation of Bax and downregulation of Bcl-2. Our results indicate that ruthenium (II) complexes with 2-(2¢-quinoly) benzimidazole frameworks, especially C1 and C4, had a higher cytotoxic and apoptotic activity in human glioblastoma cells, and they should be further evaluated in detail for its anticancer properties as a new therapeutical strategy for glioblastoma. © 2024 by the authors.
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    Methylene blue treatment with zero-valent iron/pyrite/H2O2 system under static and continuous flow conditions: Reaction mechanism and toxicity evaluation
    (Elsevier Science Inc, 2024) Oral, Ozlem; Arslan, Sevki; Dogan, Nazime Mercan; Yildiz, Ilker; Kantar, Cetin; Abdelsalam, Amine Hafis; Kuzucu, Volkan
    Laboratory batch and continuous flow studies, coupled with surface and toxicity analysis, were performed to evaluate methylene blue treatment by heterogeneous Fenton process using zero-valent iron (ZVI) and pyrite as co-catalyst. The use of pyrite in batch reactors significantly enhanced methylene blue treatment by the ZVI/H2O2 system because of improved iron redox cycling. The continuous-flow experiments revealed that the reactor performance increased in the order of: ZVI/H2O2 < pyrite/H2O2 < ZVI/pyrite/H2O2 under dynamic flow conditions. The methylene blue treatment by the ternary ZVI/pyrite/H2O2 system was described by an initial degradation of methylene blue with *OH radicals, followed by the adsorption and/or co-precipitation of degradation intermediates with some spherical particles. The surface analysis showed that these spherical particles formed, even at pH less than 4. The genotoxicity and cytotoxicity tests performed on mouse embryonic fibroblast (3 T3-L1) and human embryonic kidney (HEK293) cell lines showed that the Fenton treatment of methylene blue using the ZVI/pyrite/H2O2 system resulted in the formation of degradation species with much lower toxicity levels relative to methylene blue. Moreover, the Fenton degradation species of methylene blue significantly enhanced the metabolic activity of several bacterial strains, including E. coli ATCC 8739 and P. aeruginosa PAO1.
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    Modulatory role of GSTM1 null genotype on the frequency of micronuclei in pesticide-exposed agricultural workers
    (Sage Publications Inc, 2016) Tumer, Tugba Boyunegmez; Savranoglu, Seda; Atmaca, Pelin; Terzioglu, Gulsurn; Sen, Alaattin; Arslan, Sevki
    In this study, we aimed to investigate the extent of genotoxic risk and the association between null GSTM1/GSTT1 and GSTP1 Ile105Val variants and cellular DNA damage, as measured by micronucleus (MN) assay in a group of agricultural workers from Denizli, Turkey. Peripheral blood samples were collected from 116 subjects, including 58 workers who were occupationally exposed to pesticides and 58 healthy unexposed controls. The MN frequencies of each individual were assessed by cytokinesis-blocked micronuclei assays on lymphocytes. Genotypes for different GST variants were determined using polymerase chain reaction-based methods. A significant 3.4-fold increase in MN frequency was observed in workers compared with the controls (p < 0.001). Among the GST genotypes, only the GSTM1 null genotype was found to be significantly associated with an increased MN frequency in workers (p = 0.01). Individuals with a concomitant null GSTM1/GSTT1 genotype demonstrated a significant (p = 0.01) increase in MN frequency compared with those with functional isozymes in the exposed worker group. The association of the GSTM1 null genotype with higher MN frequency suggests that it may be a modifier of genotoxic risk in individuals exposed to pesticides and may thus be a candidate susceptibility biomarker for human biomonitoring studies.