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Öğe A comparative evaluation of hematological and biochemical parameters between the Italian mullet Mugil cephalus (Linnaeus 1758) and the Turkish mullet Chelon auratus (Risso 1810)(Tubitak Scientific & Technological Research Council Turkey, 2020) Fazio, Francesco; Saoca, Concetta; Acar, Ümit; Tezel, Rifat; Çelik, Murat; Yılmaz, Sevdan; Kesbiç, Osman Sabri; Yiğit, MuratThis study aimed to carry out a comparative evaluation of the hematological profile (erythrocyte count, hemoglobin concentration, hematocrit, and Wintrobe index parameters such as mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration), in addition to certain biochemical parameters (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, lactate dehydrogenase, scrum total protein, albumin, glucose, total cholesterol, and triglycerides), in the Italian mullet Mugil cephalus (Linnaeus 1758) and the Turkish mullet Chelon auratus (Risso 1810). Accordingly, two groups of fish were used in this study: 30 flathead grey mullets, M. cephalus, caught in Lake Faro, Italy, and 30 golden grey mullets, C. auratus, caught in the estuarine channel system of Keycegiz-Dalyan, Turkey. Statistical evaluations (unpaired t-test) revealed that there were no significant differences in weight or total length values between the two mullet groups. However, the differences between all the evaluated blood parameters (except alanine aminotransferase) between the Italian and the Turkish mullet were significant (P < 0.0001). The present study demonstrated that there were significant hematological and biochemical differences between the Italian (M. cephalus) and the Turkish (C. auratus) mullet. Our findings also contribute to expanding the knowledge on the hematology and biochemistry of two different species of mullet originating from two different habitats. Since the evaluation of blood parameters represents an essential tool in examining the effects of environmental conditions on fish physiology and, consequently, on fish health status, further research in this field is encouraged and would be very useful.Öğe Alterations in blood parameters, DNA damage, oxidative stress and antioxidant enzymes and immune-related genes expression in Nile tilapia (Oreochromis niloticus) exposed to glyphosate-based herbicide(Elsevier Inc., 2021) Acar, Ümit; İnanan, Burak Evren; Navruz, Fahriye Zemheri; Yılmaz, SevdanIn this study, effects of different concentrations (0, 5, 10, 20, 30, and 40 mg/L) of glyphosate-based herbicide (GBH) on Nile tilapia (Oreochromis niloticus) were investigated after a 14-day of exposure. After determination of LC50 value, effects of GBH on hematological and serum biochemical parameters in blood samples, DNA damage, lipid peroxidation and catalase activity in liver tissues, expression levels of antioxidant enzyme-related genes (SOD, CAT, GPx, and GST) and immune-related genes (TGF-β, TGF-α and IL1-β) were evaluated. The LC50 value has been found as 44.4 mg/L for GBH. GBH exposure at all concentrations caused alterations in blood parameters. GBH induced oxidative stress in liver and DNA damage in blood. Antioxidant enzyme-related genes were significantly up-regulated to suppress oxidative stress. On the other hand, the expression levels of immune-related genes decreased in fish exposure to particularly ≥20 mg/L GBH.Öğe Comparison of milt and blood parameters including testosterone and gonadotropins levels of sex-reversed female rainbow trout (Oncorhynchus mykiss) by different steroid hormones(John Wiley & Sons Ltd, 2021) İnanan, Burak Evren; Acar, ÜmitAll-female progeny is preferable in the reproduction of rainbow trout. Using sex-reversed (SR) female rainbow trout by steroid hormones as broodstock in the reproduction is the most appropriate way to all-female progeny. This study compared mature SR female rainbow trout by ethisterone (ET), 11β-Hydroxyandrostenedione (HD) and 17α-Methyltestosterone (MT) in terms of spermatological parameters and health status, for the first time. Spermatozoa concentration, motility characteristics, seminal plasma pH, testosterone and gonadotropins in seminal plasma and blood serum, blood parameters of SR females were evaluated, comparing with testicular and stripped milt, and blood from normal males (NM). The SR females by HD had greater gonadosomatic index and better motility characteristics compared with other groups. Also, blood parameters of this group were more similar to those in NM. The SR females by ET had the lowest haematocrit and the highest glucose and activities of the enzymes compared with the other groups. The measured hormones were significantly the lowest in both seminal plasma and serum of the ET group. These findings suggested that using of HD for obtaining SR female should be preferred due to having the better quality spermatozoa and health status.Öğe CONSTRUCTION, ASSEMBLY AND SYSTEM DEPLOYMENT OF A FISH CAGE WITH COPPER ALLOY MESH PEN: CHALLENGING WORK LOAD AND ESTIMATION OF MAN-POWER(2018) Yiğit, Murat; Osienski, Michael; Decew, Judson; Çelikkol, Barbaros; Kesbiç, Osman Sabri; Karga, Mustafa; Acar, ÜmitIn the present study, a 150 cubic m net pen was designed as part of a collaborative research effort betweenthe International Copper Association (ICA-USA), the University of New Hampshire (UNH-USA) and Cannakale Onsekiz Mart University (COMU-Turkey) in August 2011. The fish cage was developed to supportthe creation of a small scale demonstration farm, located in the Strait of Canakkale, off the coast of Guzelyalitown in Turkey. The surface gravity-type, octagonal shaped fish cage was designed to have a diameter of 6m and a copper alloy mesh chamber depth of 5 m. The present study details the cage construction and systemdeployment of one fish cage utilized a chain link mesh net chamber with a copper alloy developed by Wieland-Werke in Germany, with reference to work load challenge and estimation of man-power necessary forthe partial and total work efforts. As a conclusion, one cage equipped with copper-alloy mesh pen was broughtto a final shape with the net chamber assembled and attached to the cage frame in 3 days and 90 man-hours.The HDPE (high density polythylene) cage frame was assembled by an outside company, therefore detail ofthe main cage frame is not discussed in this paper.Öğe Dietary effect of grape (Vitis vinifera) seed extract mitigates hepatic disorders caused by oxidized fish oil in rainbow trout (Oncorhynchus mykiss)(Springer Science and Business Media B.V., 2023) Terzi, Funda; Demirci, Beste; Acar, Ümit; Yüksel, Süleyman; Salum, Çagatay; Erol, Hüseyin Serkan; Kesbiç, Osman SabriThe major goal of this study was to determine the effect of grape seed extract (GSE) on liver damage in rainbow trout (Oncorhynchus mykiss) that was caused by the consumption of dietary oxidized fish oil (OFO). Rainbow trout were fed six different experimental diets coded OX-GSE 0 (OFO diet), OX-GSE 1 (OFO and 0.1% GSE), OX-GSE 3 (OFO and 0.3% GSE), GSE 0 (fresh fish oil and 0.0% GSE), GSE 1 (fresh fish oil and 0.1% GSE), and GSE 3 (fresh fish oil and 0.3% GSE) for 30 days. The lowest % hepatosomatic index (HSI) result was calculated in fish fed with OX-GSE 0 and the highest HSI was determined in fish fed with GSE 1 diets (p < 0.05). Histopathologically, hydropic degeneration in hepatocytes significantly increased OX-GSE 0 and GSE 3 compared to GSE 1 diets (p < 0.05). Deposition of lipid droplets in hepatocytes was significantly increased in OX-GSE 0 and OX-GSE 3 groups than others (p < 0.05). Liver biochemistry parameters such as superoxide dismutase (SOD), glutathione (GSH), and malondialdehyde (MDA) were significantly affected by OX and GSE treatments (p < 0.05). There were significant differences in alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) among the liver enzymes analyzed in serum in OX and GSE (p < 0.05), meanwhile no difference was observed in lactate dehydrogenase (LDH) values between groups (p > 0.05). In conclusion, liver biochemistry and histopathology of rainbow trout consuming diets containing oxidized fish oil were negatively affected. However, it was determined that the supplementation of 0.1% GSE to the diet had a significant ameliorative role in these adverse effects.Öğe Does dietary incorporation level of pea protein isolate influence the digestive system morphology in rainbow trout (Oncorhynchus mykiss)?(Wiley, 2021) Demirci, Beste; Terzi, Funda; Kesbiç, Osman Sabri; Acar, Ümit; Yılmaz, Sevdan; Kesbiç, Fevziye IşılIn the present study, fish meal (FM) was replaced by pea (Pisum sativum) protein (PP) in diet for Rainbow trout (Oncorhynchus mykiss) at levels of 0% (PP0), 25% (PP25), 50% (PP50), 75% (PP75) and 100% (PP100), and the effect of dietary PP level on the digestive system tracts and liver was investigated by micromorphological and histopathological evaluations. Morphometric measurements (mm 100g fish(-1)) of the liver width and stomach length in rainbow trout were found to be significantly larger (p <0.05) in fish with high-level pea protein as the main protein source (PP75, PP100) compared to the low-level PP replacement group (PP25). No significant differences were found in morphometric measurements for pyloric caecum and intestines among treatment groups, whereas the number of the caecum of fish fed the PP25 diets significantly increased over the control (PP0) (p<0.05). In the histological examination of the liver, mild hydropic and vacuolar degeneration was observed in all experimental groups except PP0 and PP25. The measurements of pyloric caecum fold height, enterocyte length and width of tunica muscularis of the high-level pea protein groups of PP75 and PP100 were significantly higher (p <0.05) compared to the control group. In conclusion, 25% substitution of PP can be suggested for FM in trout diets, because the findings of the present study provided evidence that the digestive system improved by increasing the number of pyloric caecum at this replacement level.Öğe Effects of dietary Ferula elaeochytris root powder concentrations on haematology, serum biochemical parameters, spermatozoa parameters, and oxidative status in tissues of males goldfish (Carassius auratus)(Elsevier B.V., 2021) İnanan, Burak Evren; Acar, Ümit; İnanan, TüldenThis study evaluates the effects of Ferula elaeochytris, a traditional medicinal herb, root powder (FRP) concentrations (0, 0.5‰, 1‰, 0.5% and 1%) on serum biochemical parameters, hematological profiles, oxidative stress conditions in liver, muscle, gonad, testicular and stripped spermatozoa, and also spermatozoa parameters of adult male goldfish (Carassius auratus). To the best of our knowledge, this is the first time that the effects of FRP on these parameters as being associated with its essential oil contents have been determined. Some important bioactive compounds such as such as 14-β-H-Pregna, α-curcumene, limonene have been determined in FRP. Following a 60-day feeding trial, blood samples were taken and then females were introduced to tanks together with changing photoperiod and water temperature for 10 days to promote spermiation. Results showed that RBC, haemoglobin, and haematocrit levels increased in 1‰ of FRP fed fish compared control group (P < 0.05). Also serum total protein and albumin levels in this group slightly increased (P > 0.05). However, blood parameters were negatively affected in 1% of FRP. Spermatozoa parameters dramatically decreased, even spermiation success could not be achieved in some fish in fish fed with 1% of FRP. Also, 1% of FRP in diet induced oxidative stress conditions in the tissues of this group. These conditions in gonad had a different pattern than those in liver in muscle. The results revealed that dietary 1‰ of FRP could be an advantageous additive while FRP at levels of >0.5% in diet was useless for or has deleterious effects on fish health and reproduction. Based on these results, we conclude that a supplement suitable concentration and analysis of major compounds of medicinal herbs which would use should be taken into consideration when planning to use them in fish diets.Öğe Effects of dietary fish oil replacement by poppy seed oil on growth performance and fillet quality of rainbow trout (Oncorhynchus mykiss)(Wiley, 2021) Örnek, Eda; Acar, Ümit; Öğütcü, MustafaRainbow trout (Oncorhynchus mykiss) were fed replacement of fish oil with diets including poppy seed oil (PSO; 0%, 50% and 100%). At the end of the 90-day feeding period, the water holding capacity of the fillets decreased when the storage time increased. The colour and pH values were affected by both storage and diets. The hardness, springiness, gumminess and chewiness index of the fillet samples decreased as a consequence of increasing storage time, whereas the cohesiveness values of the fillets increased. The addition of PSO increased unsaturated fatty acids and reduced saturated fatty acids in the fatty acid composition of the fillets. These results demonstrated that the fatty acid (FA) composition of the rainbow trout fillets was reflecting the FA composition of dietary treatments and oils sources. The total mesophilic and psychrophilic bacteria count of the fish oil (FO), poppy seed oil/fish oil (PSO/FO) and poppy seed oil (PSO) samples was found within the legal limit on the third day of storage; however, it was found above the legal limits on the fifth day of storage. The sensory evaluation results showed that the fish fed with PSO diet were found to be non-desirable. The results of the study suggested that the blend of 50% fish oil and 50% poppy seed oil could be used as a replacer of fish oil in diets for rainbow trout to obtained the best growth performance, fillet quality and storage conditions.Öğe Effects of dietary tribulus terrestris extract supplementation on growth, feed utilization, hematological, immunological, and biochemical variables of nile tilapia Oreochromis niloticus(Israeli Journal of Aquaculture - Bamidgeh, 2014) Gültepe, Nejdet; Acar, Ümit; Sabri Kesbiç, Osman; Yılmaz, Sevdan; Yildirim, Önder; Türker, AliThe present study was conducted for 88 days to evaluate the effect of dietary Tribulus terrestris supplementation on growth performance, feed utilization, and hematological, immunological, and biochemical indices of Nile tilapia, Oreochromis niloticus. A total of 144 Nile tilapia, average body weight 2.61±0.35 g, were divided into four experimental treatments. There were three replicates of each treatment. Four isonitrogenous (40% CP) and isolipidic (10% CL) experimental diets contained T. terrestris extract at levels of 0, 200, 400 and 600 mg/kg respectively. Growth performance and feed utilization of Nile tilapia were significantly higher (p<0.05) in all treatment groups fed with T.terrestris extract supplemented diets than those fed the control diet. There were no significant differences between body composition of fish (p>0.05). Hematocrit, hemoglobin, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), and myeloperoxidase variables were not significantly affected by herb extract (p>0.05). However, mean corpuscular volume (MCV) value and lysozyme activity increased with increasing T. terrestris extract (p<0.05). Serum albumin, total protein, and triglyceride levels were not affected by T. terrestris extract. Serum globulin and glucose levels increased and cholesterol levels decreased in Nile tilapia fed with herbal supplemented diets. The results of the present study suggest that Nile tilapia fed diets containing at least 400 mg/kg T. terrestris extract enhanced growth performance, feed utilization, hematological, immunological, and biochemical indices.Öğe Effects of Essential Oil Derived from the Bitter Orange (Citrus aurantium) on Growth Performance, Histology and Gene Expression Levels in Common Carp Juveniles (Cyprinus carpio)(MDPI AG, 2021) Acar, Ümit; Kesbic, Osman Sabri; Yılmaz, Sevdan; İnanan, Burak Evren; Zemheri-Navruz, Fahriye; Terzi, Funda; Fazio, Francesco; Parrino, VincenzoThe aim of this study was to detect effects of bitter orange (Citrus aurantium) essential oilcommonly called neroli oil (NO) (0, 0.25, 0.50, 1, and 1.5% referred to as NO0 NO0.25, NO0. 05, NO1 and NO1.5, respectively) on growth performance output and expression levels of some growth-related genes in the muscle tissue and some immune-related genes in the head kidney and pathologicadifferences in digestive system organs of common carp Cyprinus carpio. The NO0.25 group had a large improvement in growth efficiency at the end of the 60-day feeding cycle. Real-time PCR (Bio RAD, USA) system was used to detect variations in gene expression levels. Furthermore, NO supplementation of up to 0.25% in muscle tissue controlled the release of growth hormone (GH) and insulin-like growth factor I (IGF-I). Furthermore, in the NO0.25 treatment category, immune response gene levels TNF-α, IL-8 and IL-1ß increased in head kidney tissue. In the histological examination of the liver and intestine, there were significant differences between fish fed with N1 and N1.5 dietsThis study confirms that dietary supplementation of NO up to 0.25% can improve common carp growth efficiency and increase the expression of genes (GH and IGF-I) related to muscle growthTNF-α, IL-8 and IL-1ß genes related to immune status, and liver and intestine histological status ocommon carp.Öğe Effects of Tank Color on Growth Performance and Nitrogen Excretion of European Seabass (Dicentrarchus labrax) Juveniles(Springer India, 2016) Kesbiç, Osman Sabri; Yiğit, Murat; Acar, ÜmitThe present study investigated feed efficiency and growth performance of juvenile Seabass (Dicentrarchus labrax) cultured in tanks with different colors. Triplicate groups of experimental fish were distributed into 12 tanks, which showed four different tank colors, i.e. red, blue, green, and yellow. All the fish in the trial received the same diet for a period of 60 days. Forty fish with an average weight of 44 g were stocked in the tank. All experimental tanks were supplied with seawater of 22 ppt salinity with temperature ranging between 17–20 °C during the course of the experiment. Tank water volume in the experimental facility of the recirculating aquaculture system (RAS) was set to 40 L. The RAS system consisted of bio-filtration, UV filtration and gravel-mechanic filtration units. Results in the present study demonstrated that growth performance and feed utilization were affected by the tank colors tested in the trial. It is concluded that the selection of appropriate tank color might affect economic benefits in aquaculture facilities. © 2014, The National Academy of Sciences, India.Öğe Erratum to: New record and rare occurrence of European eel (Anguilla anguilla) from freshwater bodies in Karaburun Peninsula (İzmir, Türkiye): Anthropogenic pressures on the fish movements(İstanbul Üniversitesi, 2022) Ağdamar, Sevan; Acar, ÜmitAfter publication of the original article [1], it came to the authors’ attention that there was an inadvertently missed information in Acknowledgement section.Öğe Evaluation of an innovative and sustainable pre-commercial compound as replacement of fish meal in diets for rainbow trout during pre-fattening phase: Effects on growth performances, haematological parameters and fillet quality traits(MDPI, 2021) Acar, Ümit; Giannetto, Alessia; Giannetto, Daniela; Kesbiç, Osman Sabri; Yılmaz, Sevdan; Romano, Alessandro; Tezel, RifatThe aim of the study was to determine the potential and sustainable use of pre-commercial product ITTINSECT™ APS V1 as a major protein source in rainbow trout (Oncorhynchus mykiss) diets. A 60-day feeding experiment was conducted to potentially use ITTINSECT as fish meal replacement in the diets of rainbow trout. Five isonitrogenous in dry matter (38% crude protein) and isolipidic (15% crude lipid) diets were produced: a control diet (fishmeal-based) (ITT0) and four experimental diets replacing fishmeal by 25 (ITT25), 50 (ITT50), 75 (ITT75) and 100 (ITT100) %, with ITTINSECT™ APS V1. Triplicate tanks, containing 15 fish each (65.81 ± 1.26 g), were hand-fed to apparent satiation twice every day during the experiment. At the end of the feeding trial, significantly higher growth performance was observed in the group fed ITTM25 and ITTM50 diets. This performance was supported by growth-related gene expressions analyzed in muscle; significantly higher GH and IGF-I genes expression levels were determined in ITT25 and ITT50 when compared to control (ITT0) (p < 0.05). While no significant differences were found between the hematology values (p > 0.05), serum total protein, globulins and glucose levels were significantly different between experimental groups (p < 0.05). In addition to this, the immune-related genes such as TNF-α, IL8 and IL1-β expression levels were determined to be significantly different (p < 0.05). In conclusion, in order to achieve the best growth performance in rainbow trout and enhance sustainable aquaculture practices, replacement of fish meal with up to 50% ITTINSECT™APS V1 in diets for rainbow trout is suggested.Öğe Evaluation of Citrus limon peels essential oil on growth performance, immune response of Mozambique tilapia Oreochromis mossambicus challenged with Edwardsiella tarda(Elsevier, 2016) Baba, Esin; Acar, Ümit; Ontaş, Canan; Kesbiç, Osman Sabri; Yılmaz, SevdanThe present study was carried on to determine the effects of Citrus limon peel essential oil as feed supplement on non-specific immune system, disease resistance against Edwardsiella tarda and growth performance in Mozambique tilapia (Oreochromis mossambicus). Fish were randomly distributed at 25 fish per 50 L aquariums and fed with commercial diets supplemented with different ratios (0% for the control group and then 0.5%, 0.75% and 1%) of limon essential oil for 60 days. At the end of the feeding trial, immunological, biochemical and biometrical parameters were assessed. Also following 60 days of feeding, the fish were injected with E. tarda and mortalities were recorded. Results showed that C. limon peel essential oil supplementation diet enhanced the Nitro blue tetrazolium (NBT) positive cell count and total white blood cell (WBC) in tilapia. At the same time a significant change was found in the lysozyme activity and myeloperoxidase activity in serum. Serum glucose, cholesterol and triglycerides decreased while total protein increased in the groups fed with C. limon essential oil supplemented diets. All C. limon peel essential oil supplemented groups showed reduced mortalities following E. tarda infection compared with the control group. The results indicated that C. limon peel essential oil supplementation could improve non-specific immune parameters and growth performance and decrease mortality rate of cultured tilapia. Statement of relevance: This study determined the effects of an Citrus limon peel essential oil on immune system and resistance to disease in tilapia. The using of essential oil in fish diet prevented tilapia partially from edwardsiellosis disease and increase survival rate also enhance the immune response in fish. (C) 2016 Elsevier B.V. All rights reserved.Öğe Evaluation of metabolic stress status in common carp (Cyprinus carpio) exposed to the fungicide folpet(Elsevier Inc., 2023) Acar, Ümit; Erden, Yavuz; İnanan, Burak Evren; Kesbiç, Osman Sabri; Yılmaz, Sevdan; Çelik, Ekrem ŞanverAmong pesticides, fungicides are the most extensively used ones in agriculture and their effects on fish health and indirectly human health are needed to evaluate. Folpet has been used for over 50 years as a fungicide across the world. The mechanism of action of folpet on non-target aquatic organisms is so poorly understood and there is no available information about potential acute toxicity of folpet and its mechanism of action in non-target aquatic organisms. With this motivation, two successive experiments were set up: first, 96 h-LC50 value of folpet for common carp (Cyprinus carpio) was determined; and second, effects of different sublethal concentrations (0, 0.025, 0.50, 0.1 and 0.15 mg/L) of folpet on hematological serum biochemical blood parameters, DNA damages, expression levels of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), and cortisol receptor (HSP70) genes were evaluated in this study. It was calculated that 0.199 mg/L of folpet was the 96 h-LC50 value. After the folpet exposure for 14 days, significant decreases in red blood cells, hemoglobin, hematocrit, serum total protein, and GST gene expression levels were observed while serum glucose, liver enzymes activities, and expression levels of CAT, SOD, GPx, HSP70 genes increased. Also, folpet induced a significant genotoxic effect on the blood cells regarding to DNA damages. Consequently, the results have shown the toxic effects of folpet even at the lowest concentration on common carp.Öğe Evaluation of Sugar Beet Leave Extracts in Goldfish (Carassius auratus) Diets: Effects on Blood and Semen Parameters(2019) İnanan, Burak Evren; Acar, ÜmitSugar beet (Beta vulgaris L.) is one of the common agricultural crops in the world. After harvesting of its roots, sugar beetleaves (SBL) become waste in the field. SBL could cause some inflammation problems in the digestive tract of cattle anddecrease tasty of feed. This study was carried out to determine the effects of three different extracts from SBL on hematologicaland blood serum biochemical parameters, and semen quality of goldfish Carassius auratus. These extracts contained proteins,essential oils and carbohydrates from SBL were added to fish feed at ratios of 5 and 20 ‰. The serum total protein, glucoseand albumin values increased while cholesterol and liver enzymes activities decreased in all SBL extract groups. Also, thehighest hemoglobin values were found in the groups fed with the supplementations of the essential oils extract. Moreover, thelowest lipid peroxidation and the highest catalase activity levels of seminal plasma were found in these groups. Thesupplementations of the essential oils extract which contains phytol and vitamin E improved blood parameters and increasedthe motility parameters as well.Öğe Extract of Moringa oleifera Leaves: Possible Useful Additives to Enhance Reproductive and Growth Performance of Male Oreochromis niloticus(Central Fisheries Research Institute, 2025) Naz, Saira; Habib, Syed Sikandar; Acar, Ümit; Majeed, Saima; Kesbiç, Osman Sabri; Ullah, Mujeeb; Khayyam, KhayyamThe current study aimed to test Moringa oleifera leaves extract on the growth and reproductive performance of male Nile tilapia (Oreochromis niloticus). We purchased 420 healthy Nile tilapia (60.32±3.13 g) from the local fish farm for the feeding experiment. The fish were divided in triplicates into seven groups, including the control group, with each group consisting of 60 fish species (20 per glass aquarium). They were maintained in glass aquariums measuring 60×40×35 cm throughout the 60-day feeding trial. Experimental diets, incorporating various concentrations of M. oleifera extracts (0% 2%, 4%, 6%, 8%, 10%, and 12%), were administered to the fish at 3% of their body weight. The results revealed that experimental diets significantly (P<0.05) increased the growth and reproductive performance of the Nile tilapia compared to the control group. M. oleifera significantly (P<0.05) enhanced the levels of testosterone and luteinizing hormones. Furthermore, there was a progressive increase in the overall fertility rate with increasing concentrations of the plant extract. However, the overall performance of the fish decreased with increasing concentrations beyond 12%. The current study concluded that M. oleifera extract can be used to increase the fecundity and growth rate of the fish. © 2025, Central Fisheries Research Institute. All rights reserved.Öğe Glifosat Bazlı Herbisitin Erkek Nil Tilapiyası (Oreochromis niloticus) Üreme Dokuları ve Sperm Hücreleri Üzerine Etkileri(Mevlüt AKÇURA, 2022) Acar, Ümit; İnanan, Burak Evren; Navruz, Fahriye Zemheri; Yılmaz, SevdanGlifosat bazlı formülasyonlar, dünyada en yaygın kullanılan herbisit tarım ilaçlarındandır. Bu çalışmada, su kaynaklı glifosatın erkek Nil Tilapyası (Oreochromis niloticus) testis, sperm kanalı, testiküler ve sağım yolu ile elde edilmiş spermatozoa örnekleri üzerine etkileri belirlenmiştir. Bu amaçla, balıklar 14 gün boyunca 0, 5, 10, 20, 30 ve 40 mg/L glifosat içeren deneme akvaryumlarında tutulmuşlardır. >10 mg/L glifosatın, sağım yoluyla ve testislerden alınmış olan spermatozoa örneklerinin motilite, canlılık süresi ve vitalite değerlerini azaltıcı etkisi gözlemlenmiştir. 40 mg/L glifosat grubunda hem testiküler hem de sağım yoluyla elde edilmiş spermatozoa örneklerinde en düşük vitalite değerleri saptanmıştır. >10 mg/L üzerindeki glifosat maruziyetinin, spermatozoa hücrelerinin oksidatif dengesinde değişimlere yol açtığı belirlenmiştir. Ayrıca, testis ve sperm kanalı dokularında glifosat etkisi ile oluşan lipid peroksidasyon seviyelerindeki azalmaların, kontrol grubuna alınan testis örneği haricinde istatistiki açıdan önemli farklar göstermediği saptanmıştır. Katalaz aktivitesi ise kontrol grubuna kıyasla >5mg/L glifosat gruplarında özellikle testis dokularında artış göstermektedir. Sperm kanalı dokusundaki oksidatif cevap, testis dokusundan farklı olduğu belirlenmiştir. Sonuç olarak, bu çalışma ile glifosat bazlı yaygın olarak kullanılan ticari bir herbisitin, erkek Nil Tilapyası balığı üreme sistemi üzerine etkileri gösterilmiştir. Ortamda bulunan özellikle 5 mg/L’den fazla olan glifosat konsantrasyonun spermatozoa parametrelerine olumsuz yansıdığı saptanmıştır. Bununla birlikte daha yüksek konsantrasyonların bu herbisitin üreme sistemi dokularındaki oksidatif stres koşullarını etkileyebildiği gösterilmiştir.Öğe Gökkuşağı alabalığı (Oncorhynchus mykiss) Yemlerinde Balık Unu Yerine Kullanılan Acı Bakla Ununun (Lupinus albus) Bazı İmmünolojik Parametreler ve Gen Ekspresyon Seviyeleri Üzerine Etkisi(Çanakkale Onsekiz Mart Üniversitesi, 2018-07-17) Acar, Ümit; Karabayır, Ali; Kesbiç, Osman Sabri; Yılmaz, Sevdan; Zemheri, FahriyeBu çalışmada, gökkuşağı alabalığı (Oncorhynchus mykiss) yavru yemlerinde balık unu yerine farklı oranlarda acı bakla (Lupinus albus) unu ikamesinin (% 0(ABU0), 15(ABU15), 30(ABU30), 45(ABU45) ve 60(ABU60)) gökkuşağı alabalığı TNF-?, IL-1ß ve IL-8 genlerinin ekspresyon seviyelerindeki değişim ile bazı immünolojik parametreler üzerine etkileri belirlenmiştir. Besleme denemesinde kullanılan yemler temel protein kaynağı balık unu ve acı bakla unu olmak üzere ortalama %41,5 ham protein ve %18,1 yağ içerecek şekilde hazırlanmıştır. Besleme denemesinde kullanılan yemlerin esansiyel amino asit içeriklerinin tamamı alabalık gereksiniminden yüksek olduğu gözlenmiştir. 60 gün süren deneme sonunda her grupta 15 balıktan kan ve karaciğer doku örnekleri alınmıştır. Alınan kan örneklerinden lizozim (LİZ), myeloperoksidaz (MPO) ve respiratöri burst (RBT) aktivitesi analizleri karaciğer doku örneklerinden gökkuşağı alabalığına özgü ß-aktin, TNF-?, IL-1ß ve IL-8 genleri üzerinden moleküler tetkikler yapılmıştır. Elde edilen bulgulara göre gökkuşağı alabalığı yavru yemlerinde acı bakla unu kullanımına bağlı olarak TNF-?, IL-1ß ve IL-8 genleri ekspresyon seviyelerinin ve LİZ ve RBT aktivitelerinin artış gösterdiği (p<0,05), MPO aktivitesi üzerine etkisi olmadığı gözlenmiştir. Sonuç olarak acı bakla ununun gökkuşağı alabalığı yemlerinde %60 oranında balık unu yerine ikame edilmesinin gökkuşağı alabalıklarında bağışıklık sistemini olumlu yönde etkilediği sonucuna varılmıştır.Öğe New record and rare occurrence of European eel (Anguilla anguilla) from freshwater bodies in Karaburun Peninsula (İzmir, Türkiye): Anthropogenic pressures on the fish movements(İstanbul Üniversitesi, 2022) Ağdamar, Sevan; Acar, ÜmitObjective: Fish can be used as bio-indicators to determine whether freshwater habitat has been altered. As habitat discontinuity occurs due to the presence of mechanical barriers, species composition can be altered upstream and downstream. Since the beginning of the 1980s, the amount of European eel Anguilla anguilla entering inland waters in Türkiye and European countries has decreased significantly. Our study describes for the first time the presence of A. anguilla from the freshwater bodies in the Karaburun Peninsula. Materials and Methods: From November 2020 to August 2021, fish specimens were collected through electrofishing in one lotic system and three transitional waters of the study area. Clove oil was used to anesthetize live fish specimens after capture. Total length (TL) was measured for each sample to the nearest 1.0 mm. With a digital balance that was accurate to 0.01 g, we weighed the total body weight (W). Results: We collected four adult specimens of A. anguilla from the study area. Total lengths and body weights of the specimens were 311 mm and 62.4 g for Balıklıova Stream; 360 mm and 95.3 g for Küçükbahçe Stream; 325 mm 56.3 g for Salman Reservoir and 280 mm and 34.7 g for Yelkentaş Stream, respectively. Conclusion: We could not discuss our findings since no study has been done in the study area before, but it is thought that the low number of individuals caught may be related to the habitat degradation and different types of constructions at the stream mouths. The construction of fishways suitable for the study area can be a start to solving the distribution problem of this species.