Yazar "Özcan, Burcu" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim
    Öğe
    A novel and disposable GP- based impedimetric biosensor using electropolymerization process with PGA for highly sensitive determination of leptin: Early diagnosis of childhood obesity
    (Elsevier, 2021) Özcan, Burcu; Sezgintürk, Mustafa Kemal
    This study presents a novel, single-use electrochemical biosensor for the leptin biomarker, which may have potential use for early diagnosis of childhood obesity. The graphite paper working electrode was used for the first time in impedimetric biosensors. All immobilization procedure, investigation of the optimal parameters and characterization of biosensors were followed and evaluated using Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV). The Scanning Electron Microscope (SEM) was utilized to visualize the morphology of the electrode surface during the immobilization steps of the immunosensor. Moreover, the characterization of the interactions between anti-leptin and leptin was investigated by using Single Frequency Technique (SFI). The applicability of the designed biosensor for real serum samples was tested for clinical use. It was observed that the biosensor allows high sensitivity in the analyte detection (leptin) in real serum samples. Moreover, it was suggested that the developed biosensor presents advantages such as long shelf life (5% loss of activity after 8 weeks and 60% loss after 10 weeks), ability to determine analyte concentrations at picogram level (0.2 pg mL−1 -20 pg mL−1), low limit of detection (0.00813 pg mL− 1), reproducibility, reusability (12 times) and high sensitivity.
  • [ X ]
    Öğe
    A Practical and Single-Use ITO-Pet Based Immunosensing Platform for Detection of Tumor Necrosis Factor-Alpha Biomarker
    (2023) Özcan, Burcu
    This investigation displays a novel, practical indium tin oxide- polyethylene terephthalate (ITO-PET) based electrochemical biosensor for the Tumor Necrosis Factor-alpha (TNF?) biomarker determination. The ITO-PET electrode is a very ad - vantageous preferred semiconductive electrode material. It has a lot of great features such as easy to prepare, cheapness, flexibility, stability. It also allows determining an analyte at very low concentrations and provides a great wide concentration range for analyte analysis. Electrochemical Impedance Spectroscopy (EIS) and Cyclic Voltammetry (CV) were used for the evaluation of biosensors, including the immobilization procedure, the investigation of the optimum conditions, and the characterization of biosensors. The immunosensor's electrode surface morphology during the immobilization process was observed using a Scanning Electron Microscope (SEM). In addition, Impedance measurement at a single frequency was used to characterize anti-TNF? and TNF? interactions (SFI). The clinical effectiveness of the developed biosensor was investiga - ted by testing it with real human serum samples. Moreover, the fabricated immunosensor presents long shelf life, analysis of the antigen concentrations at picogram level (0.02 pg mL-1 -2.56 pg mL-1), reproducibility, reusability and high sensitivity
  • Küçük Resim
    Öğe
    Highly Sensitive and Single-Use Biosensing System Based on a GP Electrode for Analysis of Adiponectin, an Obesity Biomarker
    (Amer Chemical Soc, 2021) Özcan, Burcu; Sezgintürk, Mustafa Kemal
    This study presents a disposable, novel, and sensitive biosensing system to determine adiponectin, an obesity biomarker, in real human serum. The graphite paper (GP) working electrode is a new material for impedimetric biosensors. In the literature, there is no study in which this electrode is used in impedance-based biosensors for adiponectin detection. Sensitive and useful techniques, such as electrochemical impedance spectroscopy and cyclic voltammetry, were utilized for investigation of the modification of the GP electrode surface and optimization and characterization of the constructed biosensor. The single frequency impedance technique was used to study the interactions between antiadiponectin and adiponectin. The morphology of the electrode surface for each immobilization step was examined with scanning electron microscopy. All experimental parameters were optimized to fabricate a rapid and sensitive biosensing system. The designed biosensor presents excellent performance with a wide detection range (0.05-25 pg mL-1) and a low limit of detection (0.0033 pg mL-1) for adiponectin determination. Also, it has been demonstrated that the biosensor sensitively allows for the detection of adiponectin in human serum. The affinity of the designed immunosensor toward other proteins and components was examined in the presence of the target protein (adiponectin), leptin (100 pg mL-1), creatine kinase (50 pg mL-1), parathyroid hormone (50 pg mL-1), and d-glucose (0.5 M). The selectivity of the adiponectin biosensor resulted in high capacity to neglect the interference effect. The constructed biosensor showed good linearity, long-Term storage life (10 weeks), high reusability (18 times regenerability), and high ability to detect adiponectin concentrations at picogram levels. ©