Journal of Advanced Research in Natural and Applied Sciences (2015-Halen)

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https://hdl.handle.net/20.500.12428/33356

Listeleniyor 1 - 9 / 9

Effects of Pleurotus Eryngii (DC.) Quél. Mushroom Extracts on Cell Proliferation in Breast and Colon Cancer Cell Lines
(Çanakkale Onsekiz Mart University, 2024) Cömert Önder, Ferah; Davutlar, Gülce; Önder, Alper; Allı, Hakan; Ay, Mehmet
This study was aimed to collect Pleurotus eryngii (DC.) Quél. edible/medicinal mushroom from Çanakkale/Kumkale and to evaluate the antiproliferative effects of alcoholic extracts on breast and colon cancer cell lines. For this purpose, the Soxhlet method prepared methanol and ethanol:H2O (70:30) extracts by gradually increasing solvent polarities. Then, breast (MDA-MB-231, BT-549, BT-20, MCF-7) and colon (HT-29) cancer cell lines were treated with the extracts in increasing concentrations (0.05-0.5 mg/mL) for 48 h. In conclusion, methanol and aqueous ethanol extracts exhibited significant antiproliferative effects in cancer cell lines, according to the MTT assay. The cell viability in the triple-negative breast cancer (TNBC) MDA-MB-231 cell line was highly decreased by methanol extract at a very low concentration (0.1 mg/mL). Furthermore, methanol extract at 0.3 mg/mL reduced the percentage of cell viability in the HT-29 cell line. Aqueous ethanol extract showed antiproliferative activity in breast and colon cancer cell lines at 0.25 mg/mL concentration for 48 h applications. In addition, some bioactive components such as 4-hydroxy flavone, chrysin, and tannic acid of aqueous ethanol extract have been determined by High-Performance Liquid Chromatography (HPLC) analysis. As a result, this study may increase interest in the studies on the anti-cancer activities and the mechanisms of P. eryngii mushroom.
Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System
(Çanakkale Onsekiz Mart University, 2024) Yaşlı, Pelin Kolçak; Savranoğlu Kulabaş, Seda; Engin, Evren Doruk
Chinese hamster ovary (CHO) mammalian cell lines are widely used as cell platforms in biopharmaceutical productions. Different transfection systems are employed for the integration of the target gene cassette into the cell genome and have limitations, such as (i) the integration region in the genome, (ii) the size of the target cassette, and (iii) long selection periods for stable expression. Transposon systems can be utilized to overcome the limitations mentioned in the efficient production of commercially significant recombinant proteins. This study aims to demonstrate the differences in production potential and selection periods by using a specially designed vector system for random genome integration in CHODG44 DHFR -/- cells and the Sleeping Beauty (SB) transposon system. In this context, the optimal transfer ratio between the donor and the helper plasmid was determined for the most efficient co-transfection in the SB transposon system. According to the results, the pools obtained using the SB transposon system had titers ranging from 1300 to 2600 mg/L in 13-day fed-batch studies, while the pool obtained using the random transfer system had a titer of 0.056 mg/L. Additionally, stable cell pools obtained using the transposon system underwent selection in a short period of 52 days, compared to over 100 days for the pool obtained through random transfer. Considering all these results together, it is demonstrated that stable CHO pools obtained using the optimal SB transposon system can achieve high-efficiency monoclonal antibody production in a short period, making it an optimal production platform in the biopharmaceutical field.
Optimization of Low-Calorific Coal Application at Different Loads in 600 MW Supercritical Thermal Power Plant with the PROMETHEE-GAIA Method
(Çanakkale Onsekiz Mart University, 2024) Emir, Aykut; Motorcu, Ali Rıza; Demirören, Hülya
This study examined the 600 MW supercritical unit of a 1200 MW imported coal-fired thermal power plant in Çanakkale, Türkiye. Coal blends consisting of low-calorific domestic coal (4087 kcal/kg) and high-calorific imported coal (5954 kcal/kg) were combusted at the single mill and burner level to analyze unit parameters at different loads. Initially, input parameters, levels affecting unit parameters, and output parameters influenced by different coal types were identified and prioritized. Using criteria weights determined by the entropy method, the optimal load and domestic-imported coal blend ratio were determined using the Preference Ranking Organization Method for Enrichment Evaluation-Geometrical Analysis for Interactive Aid (PROMETHEE-GAIA) multicriteria decision-making method. The optimization study concluded that a 450 MW load with a 14.6% domestic coal feed rate is the most suitable alternative.
Seismic Risk Evaluation of Existing Reinforced Concrete Buildings: A Case Study for Çanakkale-Türkiye
(Çanakkale Onsekiz Mart University, 2024) Benek, Muhammed; Aktan, Selen
Türkiye is in an important earthquake zone, and most of the population is under seismic hazard. The high loss of lives in the past earthquakes indicates that our existing building stock is vulnerable. In this study, a field survey was carried out to determine the existing earthquake risks of reinforced concrete buildings in İsmetpaşa, Barbaros, Cevatpaşa, Kemalpaşa, Namıkkemal, Esenler, and Fevzipaşa Neighborhoods of Çanakkale city center. This study was conducted to specify the regional distribution of risky buildings to be examined by the street survey method called \"Simplified methods that can be used to determine the regional risk distribution of buildings\" in the implementation regulation of Law No. 6306. For this purpose, 585 reinforced concrete buildings were examined with this method, and the earthquake performance scores of these buildings were determined. The performance scores calculated as a result of reflecting the number of stories, earthquake hazard zone, type of structural system, and structural irregularities of the buildings were evaluated based on different parameters. It was seen that soft stories with values ranging from 50% to 100% and heavy overhangs with values ranging from 37% to 63% were the most common irregularities in existing buildings in Çanakkale.
Betonarme Çerçeve ve Kolonlarda İtme Analizi
(Çanakkale Onsekiz Mart University, 2024) Özkarabulut, Aydın; Köksal, Hasan Orhun
Bu makale, 1950'lerden bu yana betonarme çerçeve ve kolonların karmaşık davranışlarının modellenmesine yönelik devam eden deneysel ve analitik çalışmaların, günümüz yönetmeliklerindeki statik itme analizlerine olan etkileri üzerine odaklanmıştır. Bu noktada yapı elemanlarının moment eğrilik ilişkilerinin analizlere gerçekçi bir şekilde yansıtılması, davranışı belirleyici nitelik taşımaktadır. Bu çalışmada, Koksal-Erdogan tarafından önerilen çok eksenli basınç altındaki beton davranış modeli kullanılarak geliştirilen bir program aracılığıyla elde edilen moment-eğrilik ilişkileri, analizlerde kullanılmıştır. Geliştirilen yapısal analiz programı ise, tek açıklıklı ve tek katlı boş betonarme çerçeve ile ankastre tekil kolonların deneysel olarak belirlenmiş davranışlarını tahmin etmekte kullanılmıştır. Yapılan analizlerin sonucunda, deney çerçeve ve kolonlarına ait yatay deplasmanlar ve kuvvetlerin, Koksal-Erdogan modeli ile birlikte karşılaştırmalı bir şekilde Mander modelinden elde edilen moment-eğrilik diyagramları kullanılarak başarıyla tahmin edilebildiği belirlenmiştir. Makalede ayrıca, Mander modeli ile Koksal-Erdogan modeli arasında gerilme-şekildeğiştirme ilişkileri bakımından da karşılaştırmalar yapılmış ve sonuçların oldukça uyumlu olduğu ancak Koksal-Erdogan modelinin biraz daha düşük gerilme değerleri verdiği belirtilmiştir. Geliştirilen moment-eğrilik programının geçerliliğini göstermek amacıyla, literatürde bulunan SEMAp programıyla bazı karşılaştırmalar yapılmış ve iki program arasında benzer sonuçlar elde edildiği belirlenmiştir. Koksal ve Mander modellerine göre incelenen kolonlar ve çerçeve için elde edilen iç kuvvet şekil değiştirme tahminlerinin, deney sonuçlarıyla uyumlu olduğu gözlenmiştir.
Anticancer, Antioxidant, Antimicrobial Activities, and HPLC Analysis of Alcoholic Extracts of Parthenocissus quinquefolia L. Plant Collected from Çanakkale
(Çanakkale Onsekiz Mart University, 2024) Önder, Ferah Cömert; Kalın, Sevil; Maraba, Özlem; Önder, Alper; Ilgın, Pınar; Karabacak, Ersin
This study aimed to contribute to revealing the health effects of the Parthenocissus quinquefolia L. (PQ) plant by examining the biological activities of various extracts of the plant. An ethnobotanical important medicinal plant, PQ belongs to the Vine family (Vitaceae) and is known for its medicinal uses. Herein, fruit and leaf alcoholic extracts of the plant exhibited anticancer activity on triple negative breast cancer (TNBC) cell lines, estrogen receptor positive (ER+) (MCF-7), and human colon adenocarcinoma (HT-29) cancer cell lines. Furthermore, fruit methanol extracts displayed strong radical scavenging activity with low IC50 values such as 0.51 mg/mL, 0.80 mg/mL, 0.84 mg/mL, and 1.45 mg/mL. The highest TEAC values (18.72 mM Trolox/mg, 9.90 mM Trolox/mg, and 8.41 mM Trolox/mg) were calculated for the extracts of red leaves. The antifungal activity was determined against Candida albicans as fungal pathogen. The cell apoptosis with methanol extract was obtained between 20 µg/mL and 70 µg/mL for 48 h and 72 h treatments by Hoechst 33258 staining of nuclei under a fluorescence microscopy. HPLC analysis was performed for methanol extracts to determine major bioactive components. Consequently, this plant extracts may guide to new strategies and may be uses in medicinal applications for further investigations.
Disposable Voltammetric Determination of Celestine Blue at a Pencil Graphite Electrode
(Çanakkale Onsekiz Mart University, 2024) Güneş, Mehmet
The proposed study describes a novel and disposable voltammetric sensor that designed for the sensitive determination of celestine blue (CelsB) using a pencil graphite electrode (PGE). The electrochemical characterization study of the designed sensor was performed by recording the cyclic voltammograms (CVs) and electrochemical impedance (EI) curves in 5.0 mM of Fe(CN)63-/4- including 0.10 M of KCl, and compared with the other carbon-based electrodes such as carbon paste (CPE) and glassy carbon (GCE) electrodes. The electrochemical behaviour of CelsB was examined at different carbon-based electrodes including PGE, CPE, and GCE by the cyclic voltammetric (CV) method. The recorded CVs showed that the remarkable response obtained at PGE toward oxidation of CelsB. Moreover, the PGE shows a wide linear range (4.0 − 150 μM) and detects the CelsB with a notable limit of detection (1.21 μM). In addition, the results from the interference studies proved that the PGE enables selective voltammetric determination of CelsB in presence of various species. A feasibility study for CelsB sensor was also tested on tap water and cherry apple juice samples and the recovery values obtained between 96.2 − 103 % with high precision and accuracy indicated that the PGE shows an acceptable and good applicability to real samples.
Optical and Electrical Characterization of Crystallized M:WO3 (Cu, Fe, Ni) Films in Acidic Medium
(Çanakkale Onsekiz Mart Üniversitesi, 2024) Yakar, Emin; Karaduman Er, Irmak; Sarf, Fatma
Pure and metal (M) doped [copper (Cu), nickel (Ni) and iron (Fe)] WO3 films have been produced on In:SnO2 (ITO) slides by using facile chemical bath deposition and then annealed at 500 oC for 2 h. Structural, morphological, electrical and optical properties of the produced WO3-based films were examined. Monoclinic WO3 phase were observed in all the samples, and the peak intensities were decreased by metal inclusion with heterogeneous film growth on ITO substrate. Slight shifts from defect related emission peaks (blue and green) were observed in metal-substituted WO3 samples from PL study. An optical band gap was observed to decrease in M:WO3 samples. The surface resistance values were significantly reduced by metal additives compared to its pure counterpart, especially by the inclusion of nickel ions in WO3. The results indicated that nucleus growth and thereby impurity/defect-related surfaces had a serious effect on the optical and electrical properties of M:WO3 films.
Changes in Plant Nutrient Concentration of Olive (Olea europaea L.) Leaves after Different Rates of Humic Acid Application
(Çanakkale Onsekiz Mart University, 2024) Uyanık, Sertaç; Sümer, Ali
This research was a one-year study to investigate how increasing rates of humic acid affect the nutrient elements [Nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), zinc (Zn) and copper (Cu)] of olive trees (Olea europaea) of 14 years old found in a private cultivation land located in Bozköy of Geyikli, Çanakkale (Latitude 35 S 431667, Longitude 4409876) in 2018. The humic acid used was a suspension of 15% humic and fulvic acid, which was a commercial product (Blackjak SC, pH 4-6). Different concentrations of humic acid [0 (control), 50, 100, 200, 400 mL tree-1] were applied directly to soil of 20-30 cm depth under canopy projection of trees after dissolving in 10 L of water in April. A randomized block design with four replicates and five doses was adopted on 20 trees. 200 leaf samples were taken in December from annual shoot tips as reciprocal leaf couples and nutrient element analyses were carried out. The macronutrient elements N and P significantly increased upon all humic acid applications. However, the changes in the elements K, Ca, Mg, Fe, Zn, and Mn were insignificant in all applications. Humic acid at all concentrations decreased copper in leaves. As a result, addition to routine farmer fertilization, humic acid application at the rate of 50 ml tree-1 was determined to be the sufficient dose for olive trees. Moreover, this mentioned dose is considered to be the most economical dose besides being the most suitable one.

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