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Öğe ANTIOXIDANT ACTIVITIES AND CHEMICAL COMPOSITON OF DIFFERENT EXTRACTS OF MOSSES GATHERED FROM TURKEY(Univ Agricultural Sciences & Veterinary Medicine Bucharest, 2017) Yayintas, Ozlem Tonguc; Sogut, Ozlem; Konyalioglu, Sibel; Yilmaz, Selehattin; Tepeli, BurcuRecent pharmacological investigations of bryophytes have proven that the active principles present in these plants are quite unique and having potential chemical application and antioxidant capacity. The mosses of Oxytegius tenuirostris, Eurhynchium striatum W. P. Schimper and Rhynchostegium murale (Hedw.) Schimp. were collected from different locations of Turkey, and dried and exracted with different solvents. Volatiles were isolated from the samples by solid phase microextraction. Gas Chromatography- Mass Spectrometry (GC-MS) was used to identify volatile compounds. Antioxidant activities of moss were tested by free radical scavenging activity (DPPH center dot assay), Trolox equivalent (TEAC) and Cupric Reducing Antioxidant Capacity (CUPRAC) methods. Antioxidant activities were found for mossesas 24.67-67.12 mg/g from chloroform extract, 18.83-35.83 mg/g from ethanol extract, 7.78-46.09 mg/g from methanol extract and 12.56-34.13 mg/g from water extract by DPPH center dot assay method. Antioxidan activities were found as 562.07-2060.52 mg/g from chloroform extract, 597.44-1765.77 mg/g from ethanol extract, 2506.23-7454.92 mg/g from methanol extract and 676.41-5631.23 mg/g from water extract by Trolox method. Antioxidant activities were found as 9.78-64.60 mg/g from chloroform extract, 5.62-28.37 mg/g from ethanol extract, 4.23-30.54 mg/g from methanol extract and 4.92-27.77 mg/g from water extract by Cuprac method. On the basis of the results it is suggested that the extract of mosses species determined here could be of use as an easily accessible source of natural antioxidant for the treatment.Öğe Characterization, biological activity, and anticancer effect of green-synthesized gold nanoparticles using Nasturtium officinale L.(BMC, 2024) Yayintas, Ozlem Tonguc; Demir, Neslihan; Canbolat, Fadime; Ayna, Tuelay Kilicaslan; Pehlivan, MelekBackground Nanostructured materials used have unique properties and many uses in nanotechnology. The most striking of these is using herbal compounds for the green synthesis of nanoparticles. Among the nanoparticle types used for green synthesis, gold nanoparticles (AuNPs) are used for cancer therapy due to their stable structure and non-cytotoxic. Lung cancer is the most common and most dangerous cancer worldwide in terms of survival and prognosis. In this study, Nasturtium officinale (L.) extract (NO), which contains biomolecules with antioxidant and anticancer effects, was used to biosynthesize AuNPs, and after their characterization, the effect of the green-synthesized AuNPs against lung cancer was evaluated in vitro. Methods Ultraviolet-visible (UV-Vis) spectrophotometry, scanning electron microscopy (SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), multiple analysis platform (MAP), and Fourier transform infrared (FT-IR) spectroscopy analyses were performed to characterize the AuNPs prepared from the N. officinale plant extract. Moreover, the antioxidant activity, total phenolic and flavonoid contents and DNA interactions were examined. Additionally, A549 lung cancer cells were treated with 2-48 mu g/mL Nasturtium officinale gold nanoparticles (NOAuNPs) for 24 and 48 h to determine the effects on cell viability. The toxicity of the synthesized NOAuNPs to lung cancer cells was determined by the 3-(4,5-dimethylthiazol-2-il)-2,5-diphenyltetrazolium bromide (MTT) assay, and the anticancer effect of the NOAuNPs was evaluated by apoptosis and cell cycle analyses using flow cytometry. Results The average size of the NPs was 56.4 nm. The intensities of the Au peaks from EDS analysis indicated that the AuNPs were synthesized successfully. Moreover, the in vitro antioxidant activities of the NO and NOAuNPs were evaluated; these materials gave values of 31.78 +/- 1.71% and 31.62 +/- 0.46%, respectively, in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay at 200 g/mL and values of 25.89 +/- 1.90% and 33.81 +/- 0.62%, respectively, in the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay. The NO and NOAuNPs gave values of 0.389 +/- 0.027 and 0.308 +/- 0.005, respectively, in the ferrous ion reducing antioxidant capacity (FRAP) assay and values of 0.078 +/- 0.009 and 0.172 +/- 0.027, respectively, in the copper ion reducing antioxidant capacity (CUPRAC) assay. When the DNA cleavage activities of NO and the NOAuNPs were evaluated via hydrolysis, both samples cleaved DNA starting at a concentration of 25 g/mL in the cell culture analysis, while the nanoformulation of the NO components gave greater therapeutic and anticancer effects. We determined that the Au nanoparticles were not toxic to A549 cells. Moreover, after treatment with the half-maximal inhibitory concentration (IC50), determined by the MTT assay with A549 cells, we found that at 24 and 48 h, while the necrosis rates were high in cells treated with NO, the rates of apoptosis were greater in cells treated with NOAuNPs. Notably, for anticancer treatment, activating apoptotic pathways that do not cause inflammation is preferred. We believe that these results will pave the way for the use of NOAuNPs in in vitro studies of other types of cancer. Conclusion In this study, AuNPs were successfully synthesized from N. officinale extract. The biosynthesized AuNPs exhibited toxicity to and apoptotic effects on A549 lung cancer cells. Based on these findings, we suggest that green-synthesized AuNPs are promising new therapeutic agents for lung cancer treatment. However, since this was an in vitro study, further research should be performed in in vivo lung cancer models to support our findings and to explain the mechanism of action at the molecular level.Öğe Chitosan Nanoparticles Loaded with Quercetin and Valproic Acid: A Novel Approach for Enhancing Antioxidant Activity against Oxidative Stress in the SH-SY5Y Human Neuroblastoma Cell Line(Mdpi, 2024) Canbolat, Fadime; Demir, Neslihan; Yayintas, Ozlem Tonguc; Pehlivan, Melek; Eldem, Asli; Ayna, Tulay Kilicaslan; Senel, MehmetBackground: Multiple drug-delivery systems obtained by loading nanoparticles (NPs) with different drugs that have different physicochemical properties present a promising strategy to achieve synergistic effects between drugs or overcome undesired effects. This study aims to develop a new NP by loading quercetin (Que) and valproic acid (VPA) into chitosan. In this context, our study investigated the antioxidant activities of chitosan NPs loaded with single and dual drugs containing Que against oxidative stress. Method: The synthesis of chitosan NPs loaded with a single (Que or VPA) and dual drug (Que and VPA), the characterization of the NPs, the conducting of in vitro antioxidant activity studies, and the analysis of the cytotoxicity and antioxidant activity of the NPs in human neuroblastoma SH-SY5Y cell lines were performed. Result: The NP applications that protected cell viability to the greatest extent against H2O2-induced cell damage were, in order, 96 mu g/mL of Que-loaded chitosan NP (77.30%, 48 h), 2 mu g/mL of VPA-loaded chitosan NP (70.06%, 24 h), 96 mu g/mL of blank chitosan NP (68.31%, 48 h), and 2 mu g/mL of Que- and VPA-loaded chitosan NP (66.03%, 24 h). Conclusion: Our study establishes a successful paradigm for developing drug-loaded NPs with a uniform and homogeneous distribution of drugs into NPs. Chitosan NPs loaded with both single and dual drugs possessing antioxidant activity were successfully developed. The capability of chitosan NPs developed at the nanometer scale to sustain cell viability in SH-SY5Y cell lines implies the potential of intranasal administration of chitosan NPs for future studies, offering protective effects in central nervous system diseases.Öğe Determination of antioxidant, antimicrobial and antitumor activity of bryophytes from Mount Ida (Canakkale, Turkey)(Natl Inst Science Communication-Niscair, 2019) Yayintas, Ozlem Tonguc; Yilmaz, Selehattin; Sokmen, MunevverIn this study, active components of bryophytes were extracted from the samples collected from Mount Ida (Canakkale-Turkey). Three different extraction methods (Soxhlet, supercritical fluid extraction-SFE, Soxhlet after SFE) were employed for 2016 samples while direct Soxhlet extraction was carried out for 2017 samples using methanol as extracting solvent. Initially, antioxidant activity tests of the crude extracts were performed for all the samples. Antioxidant properties were determined to be fairly weak or moderate in the majority of the species studied. Marchantia polymorpha (T1), a liverwort, showed high extraction yield and antioxidant activity. Similarly, high activity was detected in Hypnum cupressiforme (T9) and Neckera complanata (T10) species. The antioxidant capacity of the tested species often sheds light on other activities. Antimicrobial properties were also tested for all extracts obtained in 2017. Thuidium tamariscinum (T8) was positive against Acinetobacter haemolyticus ATCC 19002 bactericin; T1 and Isothceum myurum (T11) were positive against Bacillus subtilis TCC6633 bacteria and all other species were negative. Following these results, we have concentrated on the most active species and carried out antitumoral tests. Again, fractioned polar isolate of T1 was the only antiproliferative species against HeLa and A549 lung cancer cells.Öğe Determination of heavy metal pollution with environmental physicochemical parameters in waste water of Kocabas Stream (Biga, Canakkale, Turkey) by ICP-AES(Springer, 2007) Yayintas, Ozlem Tonguc; Yilmaz, Selahattin; Türkoğlu, Muhammet; Dilgin, YusufWaste water pollution of industrial areas can answer for the serious consequences of one of the most important environmental threats to the future. In this study, inductively coupled plasma-atomic emission spectrometry method (ICP-AES) is proposed to determine heavy metals (Pb, Cu, Cd, Cr, Zn, Al, Fe, Ni, Co, Mn) and major elements (Ca, Mg) in waste water of Kocabas Stream. The concentration of metals in the waste water samples taken from 9 different stations (St.) in Biga-Kocabas Stream in November 2004 (autumn period) were determined after simple pretreatment of samples by the proposed ICP-AES method. An analysis of a given sample is completed in about 15 min for ICP-AES the method. The results of heavy metals concentrations in waste water were found between 0.00001-77.69610 mg l(-1) by the ICP-AES technique. The concentrations of Pb, Cd, Cu, Zn, Cr, Al, Fe, Mn, Ni, Co, Mg and Ca 0.00001 (St.3,6,7) - 0.0087 mg l(-1) (St.9), 0.00001 (St.4-7) - 0.0020 mg l(-1) (St.8), 0.00001 (St.1,3-7,9) - 0.0041 mg l(-1) (St.2), 0.0620 (St.2) - 0.2080 mg l(-1) (St.3), 0.0082 (St.6) - 0.2290 mg l(-1) (St.8), 0.3580 (St.2) - 1.7400 mg l(-1) (St.3), 0.2240 (St.1) - 0.6790 mg l(-1) (St.3), 0.0080 (St.1) - 1.5840 mg l(-1) (St.3), 0.0170 (St.3) - 0.0640 mg l(-1) (St.2), 0.0010 (St.1,4,5,8) - 0.0080 mg l(-1) (St.3), 5.0640 (St.9) - 5.2140 mg l(-1) (St.1) and 43.3600 (St.2) - 77.6961 mg l(-1) (St.9), respectively. Also we measured environmental physicochemical parameters such as temperature, salinity, specific conductivity, total dissolved solid (TDS), pH, oxidation and reduction potential (ORP), and dissolved oxygen (DO) in the waste water at sampling stations.Öğe DNA methylation in bryophytes as a biomarker for monitoring environmental pollution(Natl Inst Science Communication-Niscair, 2022) Cicekliyurt, Meliha Merve Hiz; Yayintas, Ozlem TongucRapidly growing industrialization and increased need for transportation have led to environmental pollution, particularly heavy metals. Efficient monitoring would help planning effective strategies to curb such increasing pollution. In this context, we studied the epigenetic changes in the bryophyte Greater Fork-moss, Dicranum majus Turner so as to use to monitor the environmental stress conditions due to accumulation of heavy metals and toxic organic compounds. The hypothesis is that the DNAm (DNA methylation) signatures reflect changes in the environmental conditions, and thus could serve as an alternate monitoring tool to study environmental pollution. The vegetative form of D. majus was collected from two different geographical locations where one was near the main road (MR) and another in the forest area (FS). DNAm rate was found 10.41 +/- 2.009 and 23.37 +/- 2.94 in MR and FS, respectively (P <0.005). The only difference between the two samples were traffic related pollutants. Thus, the reuslts suggest that vehicle pollution induces epigenetic changes in bryophytes, particularly DNA methylation, and could serve as a valuable biomarker to assess pollution risk due to vehicle traffic.Öğe Investigation of Competitive and Noncompetitive Adsorption of Some Heavy Metals Ions on Leucodon sciuroides (Hedw.) Schwagr(Amer Chemical Soc, 2020) Ucarli, Okan; Yayintas, Ozlem Tonguc; Engin, Mehmet Soner; Cay, Seydahmet; Saglikoglu, Gulsen; Yilmaz, SelehattinHeavy metals are an important pollutant group. Adsorption is one of the methods used to remove heavy metals from the environment. Mosses were preferred as bio-indicators because they have the capacity to accumulate many elements by their high surface-to-volume ratio. Leucodon sciuroides (Hedw.) Schwagr. (LS) are mosses that play an important part of the ecosystem and are collected from the Ida Mountain (Kazdag) region of canakkale (Turkey). For the purpose of determining the adsorption capacity of heavy metal ion (Pb2+, Cd2+, Co2+, Ni2+, Zn2+, and Cu2+) analysis conditions, pH, contact time, and adsorbent amounts were determined and the maximum adsorption capacity was calculated with the help of the relevant isotherms. Heavy metal concentrations were determined by inductively coupled plasma-mass spectrometry. It was determined that the optimum adsorption for mosses was 30 min at pH = 6.0 (the pH at which maximum adsorption occurs). The adsorption event shows that some divalent cations fit the Freundlich isotherm and some fit the Langmuir isotherm model. A pseudo-second-order reaction best fits the kinetic data for metal ions. Among the six metal ions studied, the highest adsorption was observed in Pb2+ and Cu2+ cations. According to the competitive adsorption results, the moss has a great advantage in determining the Pb(2+ )and Cu2+ cations industrially as well as other metals and in removing other metal impurities from the environment. Also, LS is exploited as a biosorbent to remove metal ions from aqueous solutions and can be used as a biomarker.Öğe Molecular investigation of nematodes isolated from three economical fish species taken from Canakkale (Turkiye) fish market(Ege Univ, Fac Fisheries, 2023) Aldik, Ruhay; Cakir, Fikret; Yayintas, Ozlem TongucThis study was based on the molecular identification of endoparasites sampled from three economically valuable fish species caught from the waters of the Canakkale (Turkiye). Subjected samples in the study were from chub mackerel (Scomber japonicus Houttuyn, 1782), anchovy (Engraulis encrasicolus Linnaeus, 1758), and bogue (Boops boops Linnaeus, 1758) without gender discrimination. The nematode parasites obtained from the samples were sent to molecular diagnostic laboratories in alcohol and the results were interpreted. As a result of the study, Anisakis typica (Diesing, 1860) Baylis, 1920 and Anisakis pegreffii Campana-Rouget and Biocca, 1955 were isolated from the chub mackerel fish and Anisakis pegreffii species were also isolated from anchovy and bogue. This parasite was the most isolated nematode parasite among all nematodes.Öğe NEW MOSS RECORDS FROM WESTERN PART OF TURKEY(Bangladesh Botanical Soc, 2013) Yayintas, Ozlem TongucTwo species of moss namely, Onchoporus dendrophilus Hedderson & Blockeel and Sphagnum fimbriatum Wilson are reported for the first time from Turkey. Onchoporus dendrophilus is also a new report from Southwest Asia. Diagnostic characters, descriptions and illustrations were studied.Öğe Seasonal changes of antioxidant activity and DNA damage protection potential of Fontinalis antipyretica Hedw. And hypnum cupressiforme Hedw.(Parlar Scientific Publications, 2019) Yayintas, Ozlem Tonguc; Demir, NeslihanIn this study, antioxidant activity was evaluated by selected mosses DPPH, ?-carotene/Linoleic acid test, ABTS, and CUPRAC methods. This is the first time DNA damage protection effect of seasonal differences Fontinalis antipyretica Hedw. and Hypnum cupressiforme Hedw. The amount of total phenolic and flavonoids were high in F. antipyretica methanol extracts in spring season. The amount of phenolics content and flavonoids were high in F. antipyretica methanol extracts in spring season than others. F. antipyretica methanol extracts in winter showed better DPPH free-radical scavenging assay and ?-carotene/Linoleic acid assay. The highest CUPRAC and ABTS antioxidant activity was seen in winter and spring season H. cupressiforme chloroform and methanol extracts, respectively. The protection against DNA damage activity of extracts were studied by agarose gel electrophoresis method. It was observed that spring season chloroform extract of H. cupressiforme completely disintegrate DNA in 1 ?M concentration. © 2019 by PSP.Öğe SEASONAL CHANGES OF ANTIOXIDANT ACTIVITY AND DNA DAMAGE PROTECTION POTENTIAL OF FONTINALIS ANTIPYRETICA HEDW. AND HYPNUM CUPRESSIFORME HEDW.(Parlar Scientific Publications (P S P), 2019) Yayintas, Ozlem Tonguc; Demir, NeslihanIn this study, antioxidant activity was evaluated by selected mosses DPPH, beta-carotene /Linoleic acid test, ABTS, and CUPRAC methods. This is the first time DNA damage protection effect of seasonal differences Fontinalis antipyretica Hedw. and Hypnum cupressifonne Hedw. The amount of total phenolic and flavonoids were high in F. antipyretica methanol extracts in spring season. The amount of phenolics content and flavonoids were high in F. antipyretica methanol extracts in spring season than others. F. antipyretica methanol extracts in winter showed better DPPH free-radical scavenging assay and beta-carotene/Linoleic acid assay. The highest CUPRAC and ABTS antioxidant activity was seen in winter and spring season H. cupressiforme chloroform and methanol extracts, respectively. The protection against DNA damage activity of extracts were studied by agarose gel electrophoresis method. It was observed that spring season chloroform extract of H. cupressiforme completely disintegrate DNA in 1 mu M concentration.Öğe The effect of heavy and alkaline metal accFmflation on the chlorophyll content of Fontinalis antipyretica Hedw. And Marchantia polymorpha L. And Ida Mofntain(Parlar Scientific Publications, 2019) Yayintas, Ozlem Tonguc; Irkin, Latife Ceyda; Yilmaz, SelehattinFontinalis antipyretica Hedw. is specially a pollutant-sensitive indicator species in fresh water and Marchantia polymorpha L. is commonly found on moist clay or silty soils, especially on recently burned land throughout the northern hemisphere. F. antipyretica and M. polymorpha were examined to determine the quantity of metal accumulation. Samples were collected from Ayazma Stream (Ida Mountain) with similar exposure conditions in July 2017. Mosses are suitable species for the uptake of heavy metals because of their morphological and physiological properties. Because of this, studies on the use of mosses as an environmental monitoring have increased in last decades. In the present study each sample were irrigated for 72 hours with solutions 0, 02 M CuCl2, ZnCl2 and Pb(NO3)2. At the end of the experimental period, the chlorophyll concentration of F. antipyretica and M. polymorpha was decreased. There was significant reduction of the chlorophyll content for each sample due to the heavy metals accumulation. © by PSPÖğe THE EFFECT OF HEAVY AND ALKALINE METAL ACCUMULATION ON THE CHLOROPHYLL CONTENT OF FONTINALIS ANTIPYRETICA HEDW. AND MARCHANTIA POLYMORPHA L. FROM IDA MOUNTAIN(Parlar Scientific Publications (P S P), 2019) Yayintas, Ozlem Tonguc; Irkin, Latife Ceyda; Yilmaz, SelehattinFontinalis antipyretica Hedw. is specially a pollutant-sensitive indicator species in fresh water and Marchantia polymorpha L. is commonly found on moist clay or silty soils, especially on recently burned land throughout the northern hemisphere. F. antipyretica and M. polymorpha were examined to determine the quantity of metal accumulation. Samples were collected from Ayazma Stream (Ida Mountain) with similar exposure conditions in July 2017. Mosses are suitable species for the uptake of heavy metals because of their morphological and physiological properties. Because of this, studies on the use of mosses as an environmental monitoring have increased in last decades. In the present study each sample were irrigated for 72 hours with solutions 0, 02 M CuCl2, ZnCl2 and Pb(NO3)2. At the end of the experimental period, the chlorophyll concentration of F. antipyretica and M. polymorpha was decreased. There was significant reduction of the chlorophyll content for each sample due to the heavy metals accumulation.Öğe Trachycystis ussuriensis (Maack & Regel) TJ Kop. A new moss record from Turkey(Gebruder Borntraeger, 2009) Yayintas, Ozlem TongucTrachycystis ussuriensis (Maack & Regel) T.J. Kop. (Mniaccae, Bryopsida) is recorded for the first time in Turkey. The specimen was collected from Aladaglar National Park, Kayseri - Yahyali. The nearest locality to Turkey for this species is Iran.Öğe Voltammetric detection of biosorption of Co, Ni, Zn, Cd, Pb, and Cu on Hypnum cupressiforme Hedw. in aquatic media(Springer Wien, 2022) Yayintas, Ozlem Tonguc; Cay, Seydahmet; Engin, Mehmet Soner; Ucarli, Okan; Saglikoglu, Gulsen; Yilmaz, SelehattinIn this study, the concentration of six heavy metal ions biosorbed at Hypnum cupressiforme Hedw. (HC), moss determined by voltammetric methods. While determining the optimum biosorption conditions, it was found that the amount of biosorbent 0.1 g, the contact time of metal ions-mosses is 30 min. The biosorption event shows that Ni2+ and Cu2+ appropriate the Langmuir isotherm and sequence of biosorption % was explained for covalent radii of these metals. Pb2+, Cd2+, Co2+, Zn2+, appropriate the Freundlich isotherm model and sequence of biosorption % was explained for the ratio of the hydration number to the hydrated radii of these metals. Biosorption results indicated that HC can be used for removing metal ions from aqueous solutions.
