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  • Küçük Resim
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    A promising disposable biosensor for diagnosis of Rheumatoid Arthritis disease
    (Springer Nature, 2025) Uzman, Ecem; Sezgintürk, Mustafa Kemal
    Rheumatoid Arthritis (RA) is a chronic autoimmune inflammatory disease that causes joint damage. Recently, Anti-citrullinated protein antibodies (ACPA) have been accepted as biomarkers for the diagnosis of RA due to their high specificity. This study aims to develop an immunosensor for the detection of mutated citrullinated vimentin antibody (MCV), which is autoantibody in the pathophysiology of RA from the ACPA family. For this purpose, (3-Aminopropil) trietoksisilan (3-APTES) silane agent was used to form a self-assembled monolayer (SAM) on the indium tin oxide coated polyethylene terephthalate (ITO-PET) electrode due to its sensitivity, practicality and ease of use. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) measurement techniques were used in the immobilization, optimization and characterization stages and real commercial serum samples were tested for the reliability of the immunosensor. This analysis method is the first biosensor based system for the determination of MCV. By the developed immunosensor, the determination range was obtained as 0.01 pg/mL -50 pg/mL MCV with a LOD value of 7 × 10–4 pg/mL MCV. As a result, a reproducible, specific and sensitive immunosensor was fabricated. © Qatar University and Springer Nature Switzerland AG 2024.
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    A single-use electrochemical biosensor system for ultrasensitive detection of Aflatoxin B1 in rice, corn, milk, peanut, chili pepper samples
    (Academic Press Inc Elsevier Science, 2024) Demirbakan, Burçak; Tarımeri Köseer, Nur; Uzman, Ecem; Özay, Özgür; Özay, Hava; Sezgintürk, Mustafa Kemal
    Aflatoxin B1, a common food contaminant in peanuts and corn and a genotoxic carcinogen in humans poses a significant risk for hepatocellular carcinoma, making its detection crucial; this study aims to develop a label-free electrochemical biosensor using a disposable indium tin oxide polyethylene terephthalate (ITO-PET) electrode modified with 3-Aminopropyltriethoxysilane for detecting Aflatoxin B1 in real food samples. Initially, optimization steps for the proposed biosensor were conducted using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) techniques. Characterization steps such as storage capacity, regeneration, and single frequency impedance (SFI) were completed for the proposed disposable biosensor after the optimization steps. The electrochemical biosensor, based on AFB1, exhibited excellent repeatability and reproducibility. It had a broad dynamic detection range from 0.1 fg/mL to 500 fg/mL, with low limits of detection (LOD) and quantitation (LOQ) at 0.19 fg/mL and 0.65 fg/mL, respectively. Finally, the proposed AFB1-based biosensor system was applied to real food samples (rice, chili pepper, milk, corn, and peanuts) for testing and validation.
  • Küçük Resim
    Öğe
    Anti-sitrullinlenmiş protein antikorları için elektrokimyasal biyosensör geliştirilmesi ve uygulaması
    (Çanakkale Onsekiz Mart Üniversitesi, Lisansüstü Eğitim Enstitüsü, 2023) Uzman, Ecem; Sezgintürk, Mustafa Kemal
    Romatoid Artrit (RA) eklem hasarına sebep olan kronik bir otoimmün inflamatuar hastalıktır. Son zamanlarda Anti-sitrullinlenmiş protein antikorları (ACPA), RA teşhisi için yüksek özgüllükleri sebebiyle biyobelirteç olarak kabul edilmiştir. Bu tez kapsamında ACPA ailesinden olan RA patofizyolojisinde otoantikor olan tanımlanan mutasyona uğramış sitruline vimentin (Anti-MCV) antikorları tayini için immünosensör geliştirilmesi amaçlanmıştır. Bu amaçla hassas, pratik ve kullanım kolaylığı sebebiyle indiyum kalay oksit kaplı polietilen tereftalat (ITO-PET) elektrot üzerine 3-APTES silan ajanı kendiliğinden oluşan tek tabaka (SAM) için kullanılmıştır. İmmobilizasyon, optimizasyon ve karakterizasyon aşamalarında elektrokimyasal empedans spektroskopisi (EIS) ve döngüsel voltametri (CV) ölçüm teknikleri kullanılmıştır. İmmunosensörün güvenilirliği için gerçek ticari serum örneklerinde deneme yapılmıştır. Çalışma sonucunda tekrar üretilebilir, spesifik ve hassas bir immünosensör elde edilmiştir.