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    Fibrin clot adherence on cleaned and decontaminated titanium abutment surfaces: An in vitro study
    (Wiley, 2024) Ulusoy, Habibe Ozturk; Ercan, Esra; Ozatik, Orhan; Tunali, Mustafa
    Statement of ProblemOsseointegration is now primarily established, but soft tissue integration is still susceptible to failure and problematic on implant surfaces. So, implant dentistry is increasingly focusing on improving peri-implant soft tissue integration.PurposeThe present study aimed to evaluate the blood fibrin clot formation and adhesion on the abutment after cleaning and decontamination and determine the suitable abutment surface associated with fibrin clot attachment.Materials and MethodsForty-two abutments (14 per group) were used in the present study: a brand-new (BN), contaminated with biofilm (CO) and decontaminated with an enzymatic cleaner and autoclave sterilization (DEC). For a fibrin clot, 9 mL of whole human blood and abutments was centrifuged at 2700 rpm for 12 min. Clots were divided into two parts for histomorphometry and scanning electron microscopy (SEM) analysis. Twelve abutments disconnected from the clot and two not treated with blood were observed under SEM.ResultsResidual debris and biofilm were observed on the abutment surface in the CO group but not in other groups. Healthy and organized fibrin clots formed on all abutments. The fibrin extension areas are distributed uniformly in BN and DEC groups but irregularly in CO. The surface percentage of the fibrin clot extensions was 41.76% +/- 6.73, 26.99% +/- 6.40, and 37.83% +/- 9.72 for the BN, CON, and DEC groups, respectively. The blood clot-attached areas in the CO group were statistically lower than the other groups. No difference was observed between the BN and DEC groups.ConclusionsThis study confirmed that surface contamination could influence blood clot attachment on the abutment surfaces. Cleaning and sterilization can have a favorable effect on soft tissue healing on abutment surfaces.
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    Investigation of Dosage Distributions of Polyvinyl Siloxane Dental Impression Shields for Head and Neck Radiotherapy with Thermoluminances Dosimeters
    (Marmara Univ, Inst Health Sciences, 2023) Ulusoy, Habibe Ozturk; Deniz, Yesim; Aktas, Cagatay; Aygun, Esma Basak Gull
    Objective: This study aimed to assess the effects of denture materials on dose distribution on a head and neck radiotherapy-appropriate model and calculate the thickness of a stent by polyvinyl siloxane dental impression material for shielding scattered radiation from dental restorations. Methods: In the first step of the study, 5mm diameter and 5mm height of cylindrical dental material of titanium, zirconia lithium disilicate were irradiated with 6-Megavoltage photons from a clinical linear accelerator. In the second step, dental materials at the center of polyvinyl siloxane thicknesses of 5, 10, and 20mm were irradiated with 2 Gray and 10 Gray fractional doses. Measurements were made using three thermoluminescent dosimeters positioned laterally. The percentage backscattered dose and percentage dose decrease values were calculated Results: According to the result, dosages scattered from dental materials increased for samples irradiated with 2Gy; a decreased dose was reported for samples irradiated with a 10Gy. 5mm PVS samples provided higher dose attenuation than others. Regardless of dental material, it is seen that the attenuation intensities calculated from TLD-100 dosimeters ranged from 22.7 to 38,62 for 2Gy, and 10.01 to 38,87 for 10Gy. Conclusion: Dental material alters the scattered radiation. In irradiated head and neck cancer patients, a 5mm thick guard is sufficient to prevent radiation diffused from dental materials in clinical usage.