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    A novel method to overcome coat-imposed seed dormancy in Lupinus albus L. and Trifolium pratense L.
    (Hindawi Publishing Corporation, 2014) Tiryaki, Iskender; Topu, Mustafa
    We have developed a novel method to overcome coat-imposed seed dormancy in legume plants. Seeds of Lupinus albus L. and Trifolium pratense L. were stored in a freezer at -80°C for a period of time and then immediately treated with or without hot water at 90°C for 5 seconds. Germination tests were carried out in darkness at 20 ± 1.0 °C with four replications in a completely randomized design. Final germination percentage (FGP), germination rate, and synchrony of seeds were evaluated. The results showed that new approach of freeze-thaw scarification provided high percentage of germinations in white lupin (84.16%) and red clover (74.50%) seeds while control seeds had FGPs of 3.3% and 26.0%, respectively. The immediate thawing of frozen seeds in hot water for 5 seconds was found not only an effective and reliable but also the quickest seed treatment method to prevail against coat-imposed seed dormancy in legume species and may become operationally applicable to other plant species. © 2014 Iskender Tiryaki and Mustafa Topu.
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    Öğe
    Diversity Analysis of Common Vetch (Vicia Sativa L.) Lines and Cultivars Using Pairwise Combinations of Universal Rice Primers
    (International Society of Academicians, 2022) Topu, Mustafa; Tiryaki, İskender
    This study has been conducted to determine genetic diversity of the common vetch lines and cultivars by using pairwise combinations of universal rice primers (URPs). A total number of 37 URP marker pairs were tested and twenty of those provided amplicons in the common vetch genome. The pairs of amplified URP markers provided a total of 83 bands and 62 of them were determined as polymorphic and were scattered to the whole genome. The average polymorphism rate of the primers was calculated as 73.5% while the polymorphism information content (PIC) values have ranged from 0.11 to 0.47 with an average of 0.24. The phylogenetic tree constructed based on UPGMA analysis provided three main clades. Two-dimensional plot of PCA and the UPGMA analysis showed that the URP markers successfully distinguished the genetic material based on their genetic origin. In conclusion, this study revealed that the use of pairwise combinations of URP markers could have a better power to reveal the level of polymorphism in plant genome.