Yazar "Tekin, Sati Zeynep" seçeneğine göre listele

Listeleniyor 1 - 2 / 2
  • [ X ]
    Öğe
    Adropin Levels in Polycystic Ovary Syndrome Patients
    (Derman Medical Publ, 2017) Sen, Hacer; Erbag, Gokhan; Binnetoglu, Emine; Eroglu, Mustafa; Turkon, Hakan; Tekin, Sati Zeynep; Asik, Mehmet
    Aim: Polycystic ovary syndrome (PCOS) is one of the most commonly observed endocrinopathies in women of reproductive age. Women with PCOS are said to have increased classic risk factors for cardiovascular disease, hypertension, dyslipidemia, diabetes, and obesity, in addition to non-classic risk factors such as an increase in C-reactive protein (CRP), homocysteine, and tumor necrosis factor-a. Adropin is a protein thought to play a role in maintaining energy homeostasis and insulin response. The aim of our study is to investigate the relationship between levels of adropin and insulin resistance in PCOS patients with insulin resistance and an increased risk of diabetes. Material and Method: Fifty-seven female patients (30 patients with PCOS and 27 healthy control subjects) were enrolled in this study. All patient's body mass index and insulin resistance were calculated. The adropin levels were measured using commercial kits based on a competitive plasma EIA (enzyme immunoassay) method. Results: The adropin levels in the patient group were 10.79 ng/L, while the value was 13.02 ng/L in the control group, and the difference was statistically significant (p= 0.04). There was a significant negative correlation between the adropin levels and the insulin, aspartate aminotransferase (AST), triglyseride (TG), and homeostasis model assessment of insulin resistance (HOMA-IR) levels (p= 0.03, p= 0.03, p= 0.04, and p= 0.02, respectively). Discussion: In our study, the adropin level which is associated with insulin resistance, was found to be decreased in patients with PCOS. We think that it would be valuable to conduct new studies for the evaluation of adropin related clinical conditions leading to insulin resistance in patients with PCOS.
  • [ X ]
    Öğe
    Cultured corneas show dendritic spread and restrict herpes simplex virus infection that is not observed with cultured corneal cells
    (Nature Portfolio, 2017) Thakkar, Neel; Jaishankar, Dinesh; Agelidis, Alex; Yadavalli, Tejabhiram; Mangano, Kyle; Patel, Shrey; Tekin, Sati Zeynep
    Herpes simplex virus-1 (HSV-1) causes life-long morbidities in humans. While fever blisters are more common, occasionally the cornea is infected resulting in vision loss. A very intriguing aspect of HSV-1 corneal infection is that the virus spread is normally restricted to only a small fraction of cells on the corneal surface that connect with each other in a dendritic fashion. Here, to develop a comprehensive understanding of the susceptibility of human corneal epithelial (HCE) cells to HSV-1 infection, we infected HCE cells at three different dosages of HSV-1 and measured the outcomes in terms of viral entry, gene and protein expression, viral replication and cytokine induction. In cultured cells, infectivity and cytokine induction were observed even at the minimum viral dosage tested, while a more pronounced dose-restricted infectivity was seen in ex vivo cultures of porcine corneas. Use of fluorescent HSV-1 virions demonstrated a pattern of viral spread ex vivo that mimics clinical findings. We conclude that HCE cell cultures are highly susceptible to infection whereas the cultured corneas demonstrate a higher ability to restrict the infection even in the absence of systemic immune system. The restriction is helped in part by local interferon response and the unique cellular architecture of the cornea.