Yazar "Suner, Selin S." seçeneğine göre listele
Listeleniyor 1 - 5 / 5
Sayfa Başına Sonuç
Sıralama seçenekleri
Öğe Cryogel composites based on hyaluronic acid and halloysite nanotubes as scaffold for tissue engineering(Elsevier Science Bv, 2019) Suner, Selin S.; Demirci, Şahin; Yetiskin, Berkant; Fakhrullin, Rawil; Naumenko, Ekaterina; Okay, Oguz; Ayyala, Ramesh S.We present here preparation of mechanically strong and biocompatible cryogel composites based on hyaluronic acid (HA) and halloysite nanotubes (HNTs) of various compositions, and their applications as scaffold for different cell growing media. Uniaxial compression tests reveal that the incorporation of HNTs into HA cryogels leads to a similar to 2.5-fold increase in their Young moduli, e.g., from 38 +/- 1 to 99 +/- 4 kPa at a HA:HNTs weight ratio of 1:2. Although HA:HNTs based cryogels were found to be blood compatible with 1.37 +/- 0.11% hemolysis ratio at a HA:HNTs weight ratio of 1:2, they trigger thrombogenic activity with a blood clotting index of 17.3 +/- 4.8. Remarkably, HA:HNTs cryogel composites were found to be excellent scaffold materials in the proliferation of rat mesenchymal stem cells (MSC), human cervical carcinoma cells (HeLa), and human colon cancer cells (HCT116). The cell studies revealed that an increased amount of HNT embedding into HA cryogels leads to an increase of MSC proliferation. (C) Elsevier B.V. All rights reserved.Öğe Degradable poly(catechin) nanoparticles as a versatile therapeutic agent(Taylor and Francis Ltd., 2021) Suner, Selin S.; Şahiner, Mehtap; Mohapatra, Subhra; Ayyala, Ramesh S.; Bhethanabotla, Venkat R.; Şahiner, NurettinPoly(catechin) (p(CAT)) nanoparticles (NPs), 173 +/- 4 nm was prepared as a therapeutic agent with hydrolytic degradability affording sustainable CAT release over 20 d at carcinogenic conditions, pH 5.5 and 37.5 degrees C. Cell viability studies on MC38 colon cancer cells revealed the anticancer potential of p(CAT) NPs with 691 mu g/mL IC50 value while being well-tolerated by nonmalignant CCD841 CoN colon cells at 72 h incubation-time. P(CAT) NPs showed effective antioxidant capacity with 241 +/- 7 and 456 +/- 54 mu g/mL of GA equivalency of total phenol content (TPC), and total flavonoid content (TFC) values and 1.19 +/- 0.8 mu mol/g Trolox equivalent antioxidant capacity by 2,2'-Azino-bis-(3-ethylbenzothioazoline-6-sulfonic acid (ABTS(center dot+)) scavenging assay.Öğe HA particles as resourceful cancer, steroidal and antibiotic drug delivery device with sustainable and multiple drug release capability(Taylor & Francis Group, LLC, 2021) Şahiner, Nurettin; Suner, Selin S.; Kurt, Saliha B.; Can, Mehmet; Ayyala, Ramesh S.Hyaluronic acid (HA) particles with divinyl sulfone (DVS) crosslinking at 10% mole ratio (HA macromolecule repeating units) were prepared and demonstrated as versatile drug carriers with sustainable and long-term release capabilities for cancer drugs, corticosteroid, and antibiotics. Two different methods were chosen in drug loading process; encapsulation for cancer drugs, 5-fluorouracil (5FU), mitomycin C (MMC), and doxorubicin (Dox), and dual drug conjugation for anti-inflammatory glucocorticoid dexamethasone (Dex) and antibiotic ciprofloxacin (Cipro) drugs, respectively. It was demonstrated that HA particles prepared during drug encapsulation were attained smaller sizes with 833 ± 46, 867 ± 50, 728 ± 41 nm for 5FU, MMC, and Dox, respectively. Bare and drug loaded HA particles were shown to be blood compatible with the highest hemolytic ratio of 3.1 ± 0.12% for HA-Dex-Cipro conjugates and fairly good blood clotting index with minimum 71.7 ± 6.0% for MMC encapsulated HA particles. Drug release studies from HA particles indicated that depending on the types of cancer drugs, it is possible to gradually release the drug in long-term up to 300 h in linear fashions with the highest release of 9.34 ± 2.25 mg/g for 5FU. Similarly, drug conjugated HA-Dex-Cipro particles were also showed linear dual drug release up to 100 h at physiological conditions, pH 7.4 and 37.5 °C.Öğe Polyelectrolyte Chondroitin Sulfate Microgels as a Carrier Material for Rosmarinic Acid and Their Antioxidant Ability(Mdpi, 2022) Şahiner, Mehtap; Suner, Selin S.; Yılmaz, Aynur S.; Şahiner, NurettinPolyelectrolyte microgels derived from natural sources such as chondroitin sulfate (CS) possess considerable interest as therapeutic carriers because of their ionic nature and controllable degradation capability in line with the extent of the used crosslinker for long-term drug delivery applications. In this study, chemically crosslinked CS microgels were synthesized in a single step and treated with an ammonia solution to attain polyelectrolyte CS-[NH4](+) microgels via a cation exchange reaction. The spherical and non-porous CS microgels were injectable and in the size range of a few hundred nanometers to tens of micrometers. The average size distribution of the CS microgels and their polyelectrolyte forms were not significantly affected by medium pH. It was determined that the -34 +/- 4 mV zeta potential of the CS microgels was changed to -23 +/- 3 mV for CS- [NH4](+) microgels with pH 7 medium. No important toxicity was determined on L929 fibroblast cells, with 76 +/- 1% viability in the presence of 1000 mu g/mL concentration of CS-[NH4](+) microgels. Furthermore, these microgels were used as a drug carrier material for rosmarinic acid (RA) active agent. The RA-loading capacity was about 2.5-fold increased for CS-[R](+) microgels with 32.4 +/- 5.1 mu g/mg RA loading, and 23% of the loaded RA was sustainably release for a long-term period within 150 h in comparison to CS microgels. Moreover, RA-loaded CS-[R](+) microgels exhibited great antioxidant activity, with 0.45 +/- 0.02 mu mol/g Trolox equivalent antioxidant capacity in comparison to no antioxidant properties for bare CS particles.Öğe Preparation of Macroporous Carboxymethyl Cellulose Cryogels and Its Blood Compatibility(Cambridge Univ Press, 2020) Saniner, Nurettin; Suner, Selin S.; Tosunoğlu, MuratSuperporous Carboxy Methyl Cellulose (CMC) cryogels were synthesized by chemical crosslinking of linear CMC with divinyl sulfone (DVS) with different mole ratios of CMC repeating unit down to 2.5%. The morphology of macroporous CMC cryogels was visualized by optic microscope and scanning electron microscope (SEM) images. The swelling capacity and pore volume of CMC cryogels were found to increase with the decrease in the ratio of crosslinker to CMC, and the highest swelling capacity and pore volume values were 10825 +/- 1799% and 22.1 +/- 0.4 mL/g for 2.5% mole ratio of crosslinked CMC cryogels. The blood compatibility of CMC cryogels revealed that blood cells were destroyed with very low hemolysis ratio of 1.09 +/- 1% and also showed less thrombogenic activity with 80.2 +/- 5.1% blood clotting indexes.
