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    BIOCHEMICAL COMPOSITION, AND IN VITRO ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF SALVIA FRUTICOSA, AN ETHNOMEDICINAL PLANT
    (Aloki Applied Ecological Research And Forensic Inst Ltd, 2023) Bozyel, M. E.; Canli, K.; Benek, A.; Simsek, O.; Akata, I; Altuner, E. M.
    Salvia fruticosa (Anatolian sage) is a medicinal plant with a natural distribution in the Central and Eastern Mediterranean. It has ethnomedicinal uses in traditional medicine. The aim of this study was to investigate the biochemical composition and in vitro antimicrobial and antioxidant activities of S. fruticosa. The in vitro antimicrobial activity of S. fruticosa ethanol extract (SFEt) by disk diffusion method against thirty-nine bacterial (including eleven multidrug-resistant strains) and two fungal strains was examined. The determination of antioxidant activity was carried out by the DPPH method. The identification of biochemical composition was by GC-MS. Twenty-three components were identified in SFEt and the main one was D-camphor (20.27%). SFEt has antimicrobial activity against a wide range of microorganisms tested. The highest activity has been demonstrated against Staphylococcus epidermidis DSMZ 20044 (ST12) as a Gram-positive bacteria with a 21 mm zone of inhibition and Proteus vulgaris (MDR6) as a Gram-negative bacteria with a 13 mm zone of inhibition. Also, the data from Streptococcus pneumonia (MDR7) was very striking because a higher activity has been observed than fourteen positive controls. SFEt showed antioxidant activity almost as high as Ascorbic acid. These results have shown that S. fruticosa has a high antimicrobial and antioxidant potential.
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    Synthesis of Silver Nanoparticles Using Porella pinnata L. Extract and Evaluation of Biological Activity
    (Pleiades Publishing Inc, 2024) Simsek, O.; Demir, N.; Erdener, D.; Karakas, I.; Dogru, N. Hacioglu
    Developing an eco-friendly method for producing nanomaterials is an area of significant research and commercial interest owing to its numerous applications in various disciplines. This study used a simple green synthesis approach to produce silver nanoparticles (AgNPs) using Porella pinnata L. aqueous extract. Phytochemical constituents of P. pinnata were identified by Gas Chromatography and Mass Spectroscopy (GC-MS). The optical, structural, and morphological characteristics of the AgNPs were found using UV visible absorption spectroscopy (UV-Vis), Fourier transform infrared spectroscopy (FTIR), Zeta Potential, and scanning electron microscope (SEM). The AgNPs had an average size of 76-84 nm and were mostly spherical. Considering the antimicrobial and antibiofilm activity results, both aqueous extract and AgNP have significant activities against different microorganisms based on the disc diffusion, broth microdilution, and antibiofilm methods. The P. pinnata and AgNPs extracts were significantly inhibited all test culture especially Acinetobacter baumanii ATCC 19606. The agarose gel electrophoresis method showed that extract and AgNP both cleaved DNA by hydrolytic and oxidative. Although both P. pinnata and AgNP extracts have dose-dependent antioxidant activity, AgNP is more effective. Thus, green synthesis AgNPs may be a new alternative therapeutic agent for infection therapy.