Yazar "Sezginturk, Mustafa Kemal" seçeneğine göre listele

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    A novel and mass-sensitive quartz tuning fork platform for glial fibrillary acidic protein determination
    (Elsevier, 2025) Ozcan, Burcu; Uludag, Inci; Unal, Mehmet Altay; Ari, Fikret; Sezginturk, Mustafa Kemal; Ozkan, Sibel A.
    Traumatic brain injury (TBI) is a major cause of mortality and disability worldwide, and its diagnosis is still difficult. A unique blood biomarker for numerous neurological disorders, including traumatic brain injury, is glial fibrillary acidic protein (GFAP). Portable, quick, and functional equipments are crucial in fighting against neurological illnesses. Quartz Tuning Fork (QTF) biosensors are attracting great attention due to their seamless integration with miniaturized and portable devices. This study introduces a new, adaptable, affordable frequency analyzer for GFAP detection. A simple and stable process to design a QTF system which has gold surface modified with 3-mercaptopropionic acid (3-MPA) and its biocompatibility with GFAP antigen was investigated. The surface morphology of the QTF system was analyzed during its immobilization procedure using Atomic Force Microscopy (AFM). The constructed QTF sensor system demonstrated high repeatability (the standard deviation: +/- 0.12183 Hz), good reproducibility, linearity, ability to detect antigen concentrations at femtogram levels. It proved that the QTF sensor system decorated with 3-MPA could be a good choice for the sensitive determination of GFAP, according to the statistical data. The modified QTF sensor demonstrated remarkably straightforward and mass-sensitive GFAP detection. The developed QTF-based biosensor achieved an outstanding linear detection range of 1-100 fg mL-1. The responses of the designed QTF-based sensor to commercial human serum also show that it is a successful and promising system for clinical use.
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    Celiac disease biomarker quantification in human fluid microenvironment: A selective and ultrasensitive magnetosensing immunoplatform
    (Elsevier Science Sa, 2025) Aydin, Elif Burcu; Aydin, Muhammet; Sezginturk, Mustafa Kemal
    An innovative magnetosensing strategy for highly sensitive impedimetric determination of anti-tissue transglutaminase antibodies (anti-tTG) was developed and applied successfully in real serum and saliva samples. The transducer surface of the proposed immunosensor consisted of an indium tin electrode (ITO) attached with poly (3-thienylacetic acid) (PTAc)-coated magnetic nanoparticles, which provided a very useful surface for the attachment of the biological molecules. The PTAc-coated magnetic nanoparticles were held by a magnetic field on the electrode surface, and the immunological reaction was carried out on magnetic nanoparticles as a solid platform on which the tissue transglutaminase (tTG) was covalently bound. With the specific capture of anti-tTG on the tTG-immobilized surface, an impedimetric signal was measured, and the electrochemical response of this specific reaction was correlated with the anti-tTG concentration. A linear relationship between the impedimetric signal and the anti-tTG concentration was obtained over a wide range of 0.125-25 U/mL. This magnetobiosensor illustrated a stable quantitative signal to anti-tTG concentrations after 45 minutes of incubation with a limit of detection of 0.034 U/mL and a low relative standard deviation (RSD) of 3.61 %, n = 3. This immunosensor's electrochemical behaviour was thoroughly examined, with consideration given to factors including sensitivity, specificity, repeatability, reproducibility, and storage stability. Lastly, serum and saliva samples were analyzed using the biosensor, and excellent correlation was achieved between the commercial ELISA kit and the proposed immunosensor. As a result, this approach held out a lot of hope for a straightforward, affordable, and user-friendly analytical technique that would enable the label-free measurement of anti-tTG levels.
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    Early and sensitive diagnosis of celiac autoimmune disease by using carboxylic acid functionalized magnetic nanoparticles-assisted biosensing platform
    (Springer Wien, 2025) Aydin, Elif Burcu; Aydin, Muhammet; Sezginturk, Mustafa Kemal
    A novel impedimetric magneto-immunosensor based on iron oxide (Fe3O4) nanoparticles coated with 3-phosphonopropionic acid (3-PPA) (functionalized magnetic beads, or FMBs) was created for the highly sensitive and selective detection of anti-tissue transglutaminase antibody (anti-tTG) in human serum. This label-less immunosensor was introduced by magnetically attaching FMBs onto the working electrode surface with a neodium magnet. The FMBs were utilized as a sensing interface and had carboxylic acid groups for tTG molecules, which could selectively link the target anti-tTG antibody. The FMBs modification steps were carried out in microcentrifuge tubes and concentrated with magnetic force before electrochemical analyses. The specific immuno-interactions on the FMBs surface were characterized by using the electrochemical and microscopic techniques, and in the presence of anti-tTG antibodies, they were captured by tTG-immobilized magnetic beads, and significant increases were observed in impedimetric response. The magneto biosensor response was linearly related to the anti-tTG antibody level in a broad linear range of 0.125-15.62 U/mL and a low detection limit (LOD) of 0.04 U/mL. Additionally, this magneto sensor was stable, repeatable, reproducible, selective, and sensitive for determination of the anti-tTG. The commercial enzyme-linked immunosorbent assay (ELISA) method was employed to compare the responses of the suggested immunosensor in actual samples. The magneto biosensor results were in good agreement with the ELISA reference technique results. Consequently, the biosensor performance in the analysis of serum samples was acceptable.
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    MIP-based sensing strategies for the diagnosis of prostate and lung cancers
    (Elsevier, 2025) Anil, Inci Uludag; Sezginturk, Mustafa Kemal
    Prostate and lung cancer are among the most prevalent and lethal forms of the disease, and, globally, cancer is a primary cause of mortality. Early diagnosis and the application of precise biomarkers are crucial for prolonging patient lifespan and enhancing quality of life. Molecularly imprinted polymers (MIPs) are synthetic substances capable of selectively identifying and binding specific target molecules. Besides their sensitivity to biomolecules, MIPs offer significant advantages in cancer biomarker detection due to their cost-effectiveness and flexible architectures. MIP-based biosensors designed for the detection of prostate and lung cancer-specific biomarkers provide unique solutions that enhance the diagnostic process and improve its accuracy. This study aims to assess the present state of MIP-based biosensors in the detection of biomarkers for prostate and lung cancer, as well as the potential opportunities provided by this technology. This study will present an overview of potential biomarkers for lung and prostate cancer, clarify the fundamental features of MIPs, and discuss their uses in MIPbased biosensors along with prospective future applications. In this context, our review is aimed to contribute to the development of innovative methods used in early cancer diagnosis.