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    Organic-inorganic biohybrid films from wool-keratin/jellyfish-collagen/silica/boron via sol-gel reactions for soft tissue engineering applications
    (IOP Publishing, 2024) Yıldız, Safiye Nur; Sezgin Arslan, Tugba; Arslan, Yavuz Emre
    Therapeutic angiogenesis is pivotal in creating effective tissue-engineered constructs that deliver nutrients and oxygen to surrounding cells. Hence, biomaterials that promote angiogenesis can enhance the efficacy of various medical treatments, encompassing tissue engineering, wound healing, and drug delivery systems. Considering these, we propose a rapid method for producing composite silicon-boron-wool keratin/jellyfish collagen (Si-B-WK/JFC) inorganic-organic biohybrid films using sol-gel reactions. In this approach, reactive tetraethyl orthosilicate and boric acid (pKa ⩾ 9.24) were used as silicon and boron sources, respectively, and a solid-state gel was formed through the condensation reaction of these reactive groups with the keratin/collagen mixture. Once the resulting gel was thoroughly suspended in water, the films were prepared by a casting/solvent evaporation methodology. The fabricated hybrid films were characterized structurally and mechanically. In addition, angiogenic characteristics were determined by the in ovo chick chorioallantoic membrane assay, which revealed an increased vascular network within the Si-B-WK/JFC biohybrid films. In conclusion, it is believed that Si-B-WK/JFC biohybrid films with mechanical and pro-angiogenic properties have the potential to be possessed in soft tissue engineering applications, especially wound healing.
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    Synthesis of Silica-Based Boron-Incorporated Collagen/Human Hair Keratin Hybrid Cryogels with the Potential Bone Formation Capability
    (Amer Chemical Soc, 2021) Cal, Fatma; Sezgin Arslan, Tugba; Derkus, Burak; Kiran, Fadime; Cengiz, Ugur; Arslan, Yavuz Emre
    Tissue engineering and regenerative medicine have evolved into a different concept, the so-called clinical tissue engineering. Within this context, the synthesis of next-generation inorganic-organic hybrid constructs without the use of chemical crosslinkers emerges with a great potential for treating bone defects. Here, we propose a sophisticated approach for synthesizing cost-effective boron (B)- and silicon (Si)-incorporated collagen/hair keratin (B-Si-Col-HK) cryogels with the help of sol-gel reactions. In this approach, collagen and hair keratin were engaged with a B-Si network using tetraethyl orthosilicate as a silica precursor, and the obtained cryogels were characterized in depth with attenuated total reflectance-Fourier transform infrared spectroscopy, solid-state NMR, X-ray diffraction, thermogravimetric analysis, porosity and swelling tests, Brunauer-Emmett-Teller and Barrett-Joyner-Halenda analyses, frequency sweep and temperature-dependent rheology, contact angle analysis, micromechanical tests, and scanning electron microscopy with energy dispersive X-ray analysis. In addition, the cell survival and osteogenic features of the cryogels were evaluated by the MTS test, live/dead assay, immuno/histochemistry, and quantitative real-time polymerase chain reaction analyses. We conclude that the B-Si-networked Col-HK cryogels having good mechanical durability and osteoinductive features would have the potential bone formation capability. © 2021 American Chemical Society.
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    The Use of Decellularized Animal Tissues in Regenerative Therapies
    (Kafkas Univ, Veteriner Fakultesi Dergisi, 2015) Arslan, Yavuz Emre; Hiz, Meliha Merve; Sezgin Arslan, Tugba
    Human adipose-derived mesenchymal stem cells are nonhematopoietic cells found in the adipose tissue that have multipotent characteristics. Human adipose-derived mesenchymal stem cells have ability to differentiate into multiple lineages, including osteogenic, adipogenic and chondrogenic phenotypes. Because of their high degree of plasticity and ease of isolation, they have a great potential for tissue engineering and regenerative medicine applications. Tissue engineering, using cells, soluble matriks-bound factors and supporting scaffolds, is a promising approach for regeneration, repairing and replacement of malfunctioning tissues and organs. Three-dimensional scaffolds are essential to serve as an adhesive substrate for the transplanted cells and a physical support to guide the formation of new tissues or organs. Particularly, the use of extracellular matrices prepared by decellularized whole tissue and organ as three-dimensional constructs have drawn increasing attention as a tissue engineering strategy. In this context, it is expected that investigating the cellular behaviour of human adipose-derived mesenchymal stem cells on decellularized whole tissue and organ will have a positive impact on regenerative medicine. This review offers a current perspective about decellularization of animal tissues, stem cells' behaviors on obtained matrices and potential use of these matrices in human and/or animal clinic.