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    Öğe
    Chitosan Nanoparticles Loaded with Quercetin and Valproic Acid: A Novel Approach for Enhancing Antioxidant Activity against Oxidative Stress in the SH-SY5Y Human Neuroblastoma Cell Line
    (Mdpi, 2024) Canbolat, Fadime; Demir, Neslihan; Yayintas, Ozlem Tonguc; Pehlivan, Melek; Eldem, Asli; Ayna, Tulay Kilicaslan; Senel, Mehmet
    Background: Multiple drug-delivery systems obtained by loading nanoparticles (NPs) with different drugs that have different physicochemical properties present a promising strategy to achieve synergistic effects between drugs or overcome undesired effects. This study aims to develop a new NP by loading quercetin (Que) and valproic acid (VPA) into chitosan. In this context, our study investigated the antioxidant activities of chitosan NPs loaded with single and dual drugs containing Que against oxidative stress. Method: The synthesis of chitosan NPs loaded with a single (Que or VPA) and dual drug (Que and VPA), the characterization of the NPs, the conducting of in vitro antioxidant activity studies, and the analysis of the cytotoxicity and antioxidant activity of the NPs in human neuroblastoma SH-SY5Y cell lines were performed. Result: The NP applications that protected cell viability to the greatest extent against H2O2-induced cell damage were, in order, 96 mu g/mL of Que-loaded chitosan NP (77.30%, 48 h), 2 mu g/mL of VPA-loaded chitosan NP (70.06%, 24 h), 96 mu g/mL of blank chitosan NP (68.31%, 48 h), and 2 mu g/mL of Que- and VPA-loaded chitosan NP (66.03%, 24 h). Conclusion: Our study establishes a successful paradigm for developing drug-loaded NPs with a uniform and homogeneous distribution of drugs into NPs. Chitosan NPs loaded with both single and dual drugs possessing antioxidant activity were successfully developed. The capability of chitosan NPs developed at the nanometer scale to sustain cell viability in SH-SY5Y cell lines implies the potential of intranasal administration of chitosan NPs for future studies, offering protective effects in central nervous system diseases.
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    Öğe
    Synthesis, characterization and antimicrobial activity of water soluble dendritic macromolecules
    (Elsevier France-Editions Scientifiques Medicales Elsevier, 2009) Tulu, Metin; Aghatabay, Naz M.; Senel, Mehmet; Dizman, Cemil; Parali, Tezcan; Dulger, Basaran
    Several families of water soluble dendrimers were synthesized based on poly (propyleneoxide) amines (Jeffamines (R)) (PI). PI-core and branched units were constructed from both methylacrylate and ethylenediamine (P-2-P-9, and generations 0-3 with - NH2, - COOH functionalities). They were characterized by elemental analysis (HA), gel permeation chromatography (GPC) , FT-IR, H-1, and C-13 NMR. The antimicrobial activities of only water soluble compounds (P-1, P-3, P-4, P-6, P-7 and P-9) were evaluated using disk diffusion method in water as well as the minimal inhibitory concentration (MIC) dilution method against 9 bacteria. The obtained results from disk diffusion method are assessed in side-by-side comparison with those of Penicillin-g, Ampicillin, Cefotaxime, Vancomycin, Oflaxacin, and Tetracyclin, well-known antibacterial agents. The results from dilution procedure are compared with Gentamycin as antibacterial and Nystatin as antifungal. The antifungal activities are reported on five yeast cultures namely, Candida albicans, Kluyveromyces fragilis, Rhodotorula rubra, Debaryomyces hansenii, and Hanseniaspora guilliermondii, and the results are referenced with Nystatin, Ketaconazole, and Clotrimazole, commercial antifungal agents. In most cases, the compounds show broad-spectrum (Gram-positive and Gram-negative bacteria) activities that are comparatively higher or equipotent to the antibiotic and antifungall agents in the comparison tests. (C) 2008 Elsevier Masson SAS. All rights reserved.