Yazar "Sari, Ugur" seçeneğine göre listele

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    An Alternative Cereal: Forgotten a New Plant from The Past
    (Kahramanmaras Sutcu Imam Univ, 2018) Sari, Ugur; Tiryaki, Iskender
    Changing lifestyles and eating habits from the past to the present, and increasing availability of ready-to-eat foods in daily diet along with increasing health problems promted consumers to look for alternative herbal nutrients over time. Such ongoing quests have led to the development of new plant varieties and the reconsidering of existing nutrients in terms of healthy nutrition and diet quality criteria. At the same time, such searches have led to the resurgence of plant species and varieties that have been produced using traditional or primitive agricultural methods in very marginal areas of the world, where cultivation has been abandoned for a variety of reasons, or which are still underdeveloped. Since such plants, which can also be called as fashion-plants, are announced to consumers by the press, those plants are demanded by the consumers and such demand creates new markets. The rediscovery of such plant species, which seem to have been completely forgotten in the past, is expected to become even more popular in the near future if they are addressed by different aspects of today's plants, as compared to the basic plant species and varieties that make up a large part of people's daily diet around the worldwide. As a natural plant of Ethiopia, teff [Eragrostis tef (Zucc.) Trotter] is also considered as one of such fashion-plants and takes place very important role in daily diet of people of the region. The plant has recently been shown as an alternative source of carbohydrates in terms of balanced nutrition in different parts of the world. New scientific studies are causing the plant to become more recognized and to increase its importance. This study has been prepared to introduce many aspects of teff plant which may have an important agricultural potential in near future for our country.
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    Comparative Analysis of Alfalfa Mutants in Response to Drought Stress Applied After First Cutting
    (Springer, 2024) Cetin, Selcuk; Tiryaki, Iskender; Sari, Ugur; Acar, Okan
    The purpose of this study was to determine the agro-morphological, physiological, and transcriptional characteristics of ethyl methane sulfonate (EMS)-mutagenized M3 alfalfa (Medicago sativa L.) mutants grown for 24 days under water deficit conditions following the first cutting at the blooming bud stage. Drought stress generally reduced all agro-morphological parameters measured. Cutting stress significantly reduced total chlorophyll contents of all genotypes, with the exception of mutant X20. Significant changes occurred in the levels of ascorbate peroxidase (APX), lipid peroxidation (TBARS), glutathione reductase (GR), superoxide dismutase (SOD) and crude protein levels. Drought stress considerably elevated GR levels in irrigated control (C1) and unirrigated control (C2) plants. In contrast to the other genotypes studied, mutant X20's crude protein level increased significantly after the first cutting under 24 days of water deficit conditions, compared to the level found before cutting. The expression profiles of Medicago truncatula Response to Desiccation 2 (MtRD2) and Medicago sativa proline dehydrogenase (MsProDH) genes differed based on the plant genotype and duration of drought stress. Drought stress increased MsProDH gene expression in X20, X50, and C2 plants by 4.57, 14.8, and 20.65 times, respectively. The findings of the expression study indicated that the MtRD2 gene may play a key role in stress reduction rather than drought stress per se. The results of this study revealed that cutting and drought are two independent stress variables that should be evaluated separately when evaluating genotype response to both irrigated and unirrigated growing environments in alfalfa.
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    Genetic relationship and nuclear dna content variation in Tef [Eragrostis tef (Zucc.) Trotter] accessions
    (Springer, 2020) Kaya, Caglar; Tiryaki, Iskender; Sari, Ugur; Tuna, Metin
    This study was initiated to reveal genetic relationship of 25 tef (Eragrostis tef (Zucc.) accessions by using 10 SSR markers and to determine DNA content variation by using flow cytometer analysis. Ten markers produced a total of 18 alleles and 11 of those were polymorphic. The mean polymorphism rate was 66.6%. The highest polymorphism information content value was obtained from marker CNLTs370 with 0.69 while markers CNTLs11 and CNTLs133 produced monomorphic bands only. UPGMA analysis divided 25 tef genotypes into three main clades. The accessions PI193511 and PI195934 were distinctly separated from the others. No ploidy differences were determined among the 25 tef accessions. 2C mean nuclear DNA content ranged from 1.406 pg to 1.510 with mean of 1.460 pg. The results of this study indicated that SSR markers successfully determined genetic relationship of 25 tef accession although they had a low rate of polymorphism. This study also revealed that available tef related SSR markers should be optimized before use and their efficiency may vary based on tef genotypes or accessions used.
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    Genetic variation among einkorn genotypes based on gene targeted functional markers and its possible relationship with drought tolerance at seed germination stage
    (Springer, 2022) Yilmaz, Enes Gokhan; Tiryaki, Iskender; Sari, Ugur
    Background Einkorn wheat is one of the first primary genetic resources for discovery of new alleles related to biotic and abiotic stress tolerances for further genetic improvements while it became more popular especially for its native grain status as healthy food resources. Therefore, this study aims to determine germination parameters of 32 local einkorn (Triticum monococcum) genotypes collected from Kastamonu and its vicinity, Turkey under drought stress conditions, and to reveal the genetic relationship of those genotypes based on drought related gene targeted functional markers (GTFMs). Methods and results Germination test was conducted at 20 +/- 0.5 degrees C in darkness with randomized complete block design with 4 replications. Seeds placed on double filter papers in a covered glass petri dishes (80 x 15 mm) were treated with polyethylene glycol (PEG(6000)) with a -0.23 MPa. The final germination percentages (FGPs), speed and span of germination parameters were determined. Drought stress severely worsened all germination parameters measured. The genotypes SG24 and SG12 were the most drought tolerant and sensitive genotypes based on 21.1% and 64.8% of reduction rates in FGPs, respectively. Twelve GTFMs produced a total of 32 bands and 26 of them were polymorphic. The mean polymorphism ratio of the markers and average band per marker were determined as 86.31% and 2.66, respectively. The highest polymorphism information content (PIC) was obtained from AIB1 gene marker (0.875). Conclusions This study revealed that there was a significant genetic variation for drought tolerance levels of local einkorn wheat genotypes at germination stage and drought related GTFMs can be used not only to reveal genetic variation but also to distinguish the drought tolerant genotypes.
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    Improved drought tolerance of EMS mutagenized Alfalfa (Medicago sativa L.) mutants by in vitro screening at germination stage
    (Nature Portfolio, 2022) Tiryaki, Iskender; Sari, Ugur; Cetin, Selcuk; Acar, Okan
    The objectives of this study were to determine drought tolerant novel mutant of alfalfa (Medicago sativa L.) genotypes by screening EMS mutagenized 340675 M-3 seeds at germination stages in the presence of osmotic stress of 35% PEG(6000). Root growth assay provided several drought tolerant candidate mutants. Of those, 4 mutants were further evaluated at water deficit conditions applied for 24 days after the first cutting at flowering bud stage. The results revealed that mutants determined as drought tolerant at germination stage were also tolerant to water deficit conditions. Protein content and superoxide dismutase values were found to be higher in all mutants than controls. Ascorbate peroxides, glutton reductase and lipid peroxidase values varied based on the mutant genotype and duration of drought stress. Drought stress significantly changed transcriptional levels of MtP5CS, MtDehyd, MseIF-2, MtRD2 and MsNAC genes. These results indicated that in vitro screening of alfalfa mutant seeds for osmatic tolerance at germination and early seedling growth stages was successfully able to determine the drought tolerant alfalfa mutants which were also tolerant to water deficit conditions after the first cutting at flowering bud stage.