Yazar "Ozturk, Samil" seçeneğine göre listele

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    Adipose-Derived Stem Cells and Application Areas
    (Cukurova Univ, Fac Medicine, 2015) Kivanc, Mujde; Ozturk, Samil; Gokalp, Sevtap; Ozdemir, Ilhan; Tuglu, Ibrahim
    The use of stem cells derived from adipose tissue as an autologous and self-replenishing source for a variety of differentiated cell phenotypes, provides a great deal of promise for reconstructive surgery. The secret of the human body, stem cells are reserved. Stem cells are undifferentiated cells found in the human body placed in any body tissue characteristics that differentiate and win ever known to cross the tissue instead of more than 200 diseases and thus improve and, rejuvenates the tissues. So far, the cord blood of newborn babies are used as a source of stem cells, bone marrow, and twenty years after tooth stem cells in human adipose tissue, scientists studied more than other sources of stem cells in adipose tissue and discovered that. Increase in number of in vitro studies on adult stem cells, depending on many variables is that the stem cells directly to the desired soybean optimization can be performed.. We will conclude by assessing potential avenues for developing this incredibly promising field. The aim of this paper is to review the existing literature on applications of harvest, purification, characterization and cryopreservation of adipose-derived stem cells (ASCs).
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    Alpha-synuclein levels in multiple sclerosis patients with restless leg syndrome
    (Cukurova Univ, Fac Medicine, 2020) Cakina, Suat; Yucel, Selma; Polat, Cemre Cagan; Ozturk, Samil
    Purpose: The restless legs syndrome is more frequent and critical in Multiple Sclerosis patients, and it harms their general well-being and life quality. Alpha-synuclein is a synuclein protein that can have an impact on the pathway of signaling, affecting the Dopamin 2 receptor and its receptor trafficking. Studies have shown that the decrease in dopamine 2 receptor and Restless Legs Syndrome disease are correlated. This study is aimed to ascertain the alpha-synuclein level in multiple sclerosis patients with restless legs syndrome. Materials and Methods: We took blood samples from 40 multiple sclerosis patients and 20 healthy individuals. Half of the patient group had Multiple Sclerosis with restless leg syndrome. In the study groups, the alpha-synuclein level was determined by quantitative real-time polymerase chain reaction (qRT-PCR) and enzyme-dependent immunosorbent assay (ELISA). Results: Alpha-synuclein gene expression level was found or be significantly lower in restless leg syndrome patients with multiple sclerosis than the Alpha-synuclein gene expression level in the control group. Conclusion: Alpha-synuclein may have an impact on the pathogenesis of the restless leg syndrome of multiple sclerosis disease. Further investigations are required to determine the impact of alpha-synuclein in the pathogenesis of restless leg syndrome in multiple sclerosis disease.
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    Ameliorative Effects of Ulva rigida (C. Agardh, 1823) on Cadmium-induced Nephrotoxicity in Wistar Albino Rats
    (Univ Agriculture, Fac Veterinary Science, 2022) Irkin, Latife Ceyda; Ozturk, Samil
    Many organs and systems, especially the kidney, are damaged by the effect of high-dose cadmium. These functional problems vary depending on the distribution of cadmium in the body, the way it is taken, the dose and the duration. Antioxidant activities of algae are due to the polyphenols, polysaccharides, pigments and vitamins they contain. Antioxidants are effective in protecting the body against damage caused by reactive oxygen species. This study was carried out to determine the therapeutic effects of Ulva rigida extract on kidney tissue fibrosis, inflammatory inflammation and apoptosis in cadmium-induced rats. In the study, 1 mg/kg CdCl2 was injected subcutaneously to the subjects four times a week for one month (G2). Concurrently, 50 mg/kg (G3) and 100 mg/kg (G4) algae extract were given to the subjects by gavage. In staining with hematoxylin-eosin, mononuclear cell infiltration in the kidney tissues, dilatation in the tubules, shrinkage of the glomeruli and degeneration of epithelium occurred with the induction of Cd. Immunohistochemically, it was determined that TGF-beta, TNF-alpha and NF-kB immunoreactivity was high due to tubular cells undergoing inflammation and apoptosis. Histopathological data show that U. rigida extract has a protective role against kidney damage in Cd-induced rats. Algae extract was found to play a protective role against cadmium-induced kidney damage. It was also found to have an important role as a therapeutic agent against oxidative stress.
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    ANTI-APOPTOTIC AND PROLIFERATIVE EFFECT OF MODERATE EXERCISE ON TESTICULAR TISSUE IN STREPTOZOTOCIN-INDUCED RATS
    (Parlar Scientific Publications (P S P), 2022) Ozturk, Samil; Ozdemir, Ilhan; Kamalak, Zeynep; Irkin, Latife Ceyda
    DM causes damage in many tissues and organs including the reproductive system. Severe damage in the male reproductive system is an important complication of DM. The aim of this study was to examine the effects of moderate exercise on testicular tissue in streptozotocin-diabetic (STZ) rats and to investigate the possible positive effects of exercise on the histological findings. Thirty adult rats were assigned to three groups: (a) control, (b) diabetes (40 mg/kg STZ) and (c) diabetes moderate exercise. The moderate exercise group started the exercise program 3 days before the induction of diabetes. After four weeks, the testes tissues of the rats were resected. Histochemical staining of the collected tissue specimens and morphometric tests were performed. Our study showed a reduction in the testis weight in the diabetes groups. The testis tissue of the control group was normal. Despite the atrophic changes, primary spermatocytes and spermatids were available in the testis tubules of the diabetic group. In the moderate exercise group, the architecture of the seminiferous tubules was close to normal in most of the specimens and the microscopic findings were not significantly different from those of the control group. Immunohistochemical results revealed that PCNA and TUNEL positive cells were significantly increased in the diabetic groups compared to the control group (p<0.001). The findings of our study supported that exercise had a significantly favorable effect on infertility, which is a complication of diabetes. Further molecular studies are required on this subject.
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    Antiapoptotic and proliferative effect of bone marrow-derived mesenchymal stem cells on experimental Asherman model
    (Cukurova Univ, Fac Medicine, 2019) Ozturk, Samil; Sonmez, Pinar Kilicaslan; Ozdemir, Ilhan; Topdagi, Yunus Emre; Tuglu, Mehmet Ibrahim
    Purpose: We investigated the effects of stem cell therapy as an alternative to surgical methods and medical treatments in endometrial injuries in Asherman syndrome (AS). Materials and Methods: In this study, AS model was created chemically in rats. The bone marrow-derived mesenchymal stem cells isolated from the tibia and femoral bone of male individuals of the same species (BMDSC) were given to female rats with asherman syndrome and the changes in the endometrium were evaluated by histopathological parameters. Asherman + medium, Asherman + niche, Asherman + BMDSCs, Asherman + BMDSCs + niche were formed in four groups. Results: It was observed that increased endometrial thickness, gland count and vascularization and decreased fibrous areas and apoptotic cell death with regeneration in epithelium and lamina propria in treatment groups. No histopathologic changes were observed in the right uterine horns, which were evaluated as control group.. Conclusion: BMDSCs and Niche applications can contribute to the clinic by reducing the formation of adhesion within the mechanisms causing infertility. These positive results are promising in terms of transporting Asherman studies to the clinic.It has been shown that BMDSCs and Niche may contribute to the clinic by treatment with adhesion molecules in mechanisms that cause infertility.
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    Oxidative stress markers in liver in streptozocin-induced diabetic rats: effects of metformin and sitagliptin
    (Cukurova Univ, Fac Medicine, 2020) Cakina, Suat; Ozturk, Samil
    Purpose: This study aims at investigating the oxidative stress effect of antidiabetic drugs of Metformin (MET) and sitagliptin (STG) in the liver tissue of diabetic rats from streptozotocin (STZ). Materials and Methods: Thirty-five female Wistar rats (3-4 months old, weighing 200 +/- 25 g) were divided into five groups (with seven rats each) and treated as follows: control (Cont), streptozotocin alone (STZ), streptozotocin + metformin (STZ+MET), streptozotocin + sitagliptin (STZ+STG), streptozotocin + metformin + sitagliptin (STZ+MET+STG). Sitagliptin, Metformin, and combined metformin sitagliptin treated subgroups for four weeks. Malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS) levels, and oxidative stress index (OSI) ratio were measured in rat liver tissue. Besides, cells undergoing apoptotic cell death were determined using the TUNEL technique through histopathological evaluation. Results: MDA, TAS, and OSI of STZ+MET+STG administered group decreased compared to STZ. TOS of STZ+MET+STG administered group decreased compared to STZ. Conclusion: In the experimental T2D model in rats, it shows protective effect when sitagliptin is used with metformin against oxidative damage in liver tissue caused by STZ.