Yazar "Ozkilinc, H." seçeneğine göre listele

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    A NEW DISEASE OF GRASSPEA (LATHYRUS SATIVUS) CAUSED BY ASCOCHYTA LENTIS var. LATHYRI
    (Springer, 2016) Infantino, A.; Zaccardelli, M.; Costa, C.; Ozkilinc, H.; Habibi, A.; Peever, T.
    Ascochyta lentis var. lathyri is described for the first time infecting grasspea (Lathyrus sativus L.) in Italy. This fungus causes necrotic lesions on leaves and stems of grasspea plants. Fungal isolates obtained from these lesions were able to infect grasspea under controlled environmental conditions and induced symptoms similar to those observed in the field. Moreover, these isolates were not pathogenic to seedlings of nine other leguminous species including lentil. Sequence analysis of the nuclear ribosomal internal transcribed spacer, as well as fast-evolving protein-coding loci chitin synthase, translation elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase, revealed 99.6% to 100% similarity to sequences from Ascochyta lentis Vassiljevsky. Morphological analyses using elliptic fourier analysis and MANOVA demonstrated that conidial dimensions of the grasspea fungus were significantly different from those of A. lentis. Crosses between the grasspea fungus and A. lentis were successful and produced progeny with normal cultural morphology and growth rates. Hybrid status of the progeny was confirmed by segregation of mating type and microsatellite markers. These results indicate that the fungus infecting grasspea is a pathogenic and morphological variant of Ascochyta lentis and should be named Ascochyta lentis var. lathyri.
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    Alternaria Blight Infections of Pistachio Fruits in the Southeastern Anatolia Region of Turkey
    (Int Soc Horticultural Science, 2014) Can, C.; Konukoglu, F.; Ozgun, E.; Kusek, M.; Ozkilinc, H.; Aktan, A.; Citak, M.
    Pistacia vera L. is an important crop species in the Southeastern region of Turkey. Alternaria blight disease caused by Alternaria spp. has become epidemic for the last couple of years. Disease symptoms mainly develop on young fruits and infections continue until harvest. Repeated infections decrease fruit quantity and quality that in turn reduce the yield. Pistachio fruits with Alternaria blight symptoms were collected during 2010-2011 growing seasons. Infected fruits were sampled from Adiyaman, Gaziantep, Sanliurfa, Mardin, Kahramanmaras and Siirt provinces where large pistachio plantations are located. Sampling and pathogen isolations were started in April just after pollination and fruit set, and continued until October, which is the harvest time of pistachio in the Southeastern Anatolia region. Isolates obtained from tissues infected with Alternaria blight were characterized morphologically and analyzed with DNA sequences, the nuclear internal transcribed spacer region, ITS and allergen regions, ALT, for molecular confirmation. Twenty-three Alternaria spp. isolates were obtained and subjected to pathogenicity analysis on 'Ohadi' pistachios. All the Alternaria spp. isolates caused blight symptoms on mature pistachio fruits. Four isolates were found to be highly pathogenic and there was difference among isolates in virulence levels. One highly virulent isolate was used to test reactions of 'Uzun', 'Ohadi' and 'Siirt' cultivars under field conditions, and 'Uzun' was found to be the most tolerant cultivar followed by 'Siirt' and 'Ohadi'.
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    Alternaria blight infections of pistachio fruits in the southeastern anatolia region of Turkey
    (International Society for Horticultural Science, 2014) Can, C.; Konukoglu, F.; Ozgun, E.; Kusek, M.; Ozkilinc, H.; Aktan, A.; Citak, M.
    Pistacia vera L. is an important crop species in the Southeastern region of Turkey. Alternaria blight disease caused by Alternaria spp. has become epidemic for the last couple of years. Disease symptoms mainly develop on young fruits and infections continue until harvest. Repeated infections decrease fruit quantity and quality that in turn reduce the yield. Pistachio fruits with Alternaria blight symptoms were collected during 2010-2011 growing seasons. Infected fruits were sampled from Adiyaman, Gaziantep, Sanliurfa, Mardin, Kahramanmaras and Siirt provinces where large pistachio plantations are located. Sampling and pathogen isolations were started in April just after pollination and fruit set, and continued until October, which is the harvest time of pistachio in the Southeastern Anatolia region. Isolates obtained from tissues infected with Alternaria blight were characterized morphologically and analyzed with DNA sequences, the nuclear internal transcribed spacer region, ITS and allergen regions, ALT, for molecular confirmation. Twenty-three Alternaria spp. isolates were obtained and subjected to pathogenicity analysis on 'Ohadi' pistachios. All the Alternaria spp. isolates caused blight symptoms on mature pistachio fruits. Four isolates were found to be highly pathogenic and there was difference among isolates in virulence levels. One highly virulent isolate was used to test reactions of 'Uzun', 'Ohadi' and 'Siirt' cultivars under field conditions, and 'Uzun' was found to be the most tolerant cultivar followed by 'Siirt' and 'Ohadi'.
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    Contrasting species boundaries between sections Alternaria and Porri of the genus Alternaria
    (Wiley, 2018) Ozkilinc, H.; Rotondo, F.; Pryor, B. M.; Peever, T. L.
    The fungal genus Alternaria comprises a large number of asexual taxa with diverse ecological, morphological and biological modes ranging from saprophytes to plant pathogens. Understanding the speciation processes affecting asexual fungi is important for estimating biological diversity, which in turn affects plant disease management and quarantine enforcement. This study included 106 isolates of Alternaria representing five phylogenetically defined clades in two sister sub-generic groups: section Porri (A.dauci, A.solani and A.limicola) and section Alternaria (A.alternata/tenuissima and A.arborescens). Species in section Porri are host-specific while species in section Alternaria have wider host ranges. For each isolate, DNA sequences of three genes (Alt a1, ATPase, Calmodulin) were used to estimate phylogenies at the population and species levels. Three multilocus haplotypes were distinguished among A.dauci isolates and only one haplotype among A.solani and A.limicola isolates, revealing low or no differentiation within each taxon and strong clonal structure for taxa in this section. In contrast, 37 multilocus haplotypes were found among A.alternata/tenuissima isolates and 21 multilocus haplotypes among A.arborescens isolates, revealing much higher genotypic diversity and multiple clonal lineages within taxa, which is not typical of asexual reproducing lineages. A species tree was inferred using a Yule Speciation model and a strict molecular clock assumption. Species boundaries were well defined within section Porri. However, species boundaries within section Alternaria were only partially resolved with no well-defined species boundaries, possibly due to incomplete lineage sorting. Significant association with host specificity seems a driving force for speciation.
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    Population structure and reproductive mode of Didymella fabae in Syria
    (Wiley, 2015) Ozkilinc, H.; Thomas, K.; Abang, M.; Peever, T. L.
    Didymella fabae is a highly destructive fungal pathogen of faba bean (Vicia faba) that represents a significant yield-limiting biotic constraint in all locations where the crop is grown. However, nothing is known about the population structure of this pathogen anywhere in the world. Population genetic analyses employing 18 sequence-tagged microsatellite (STMS) markers covering eight genetic linkage groups and a PCR-based mating type marker were used to elucidate the genetic structure and reproductive mode of the pathogen in three populations in Syria. High gene diversity within populations but low genetic differentiation among populations was observed and the entire collection of isolates was assigned to a single genetic population using a Bayesian clustering algorithm. Independent analyses were performed based on four unlinked sets of STMS markers to infer reproductive mode. A simulation approach was used to estimate which of the repeated multilocus genotypes were probably the result of asexual reproduction and should be clone-corrected. A 1:1 ratio of mating types could not be rejected in any clone-corrected population, probably due to small sample sizes. Likewise, frequency of clones and sample size, but not marker linkage, had strong effects on multilocus gametic disequilibrium. The null hypothesis of random mating was rejected in the majority of populations for both non-clone-corrected and clone-corrected samples and with four sets of unlinked markers indicating a predominance of asexual reproduction in these populations. This represents the first detailed screening of clonal and genetic composition of D. fabae populations in Syria.
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    Species diversity, mating type assays and aggressiveness patterns of Monilinia pathogens causing brown rot of peach fruit in Turkey
    (Springer, 2020) Ozkilinc, H.; Yildiz, G.; Silan, E.; Arslan, K.; Guven, H.; Altinok, H. H.; Altindag, R.
    Brown rot disease caused by fungal species from theMoniliniagenus has recently been observed as one of the most important limiting factors for yield and quality of peach fruits in Turkey. During June and July of 2018, field trips to different peach orchards were performed in six provinces located in four different geographical regions of Turkey. One hundred and twenty-nine isolates were obtained from the diseased fruits, and of those, 109 were identified asMonilinia fructicola, whereas 20 wereM. laxaaccording to the species-specific molecular markers. Mating types of the isolates were detected by PCR assays using primers designed in this study. Each isolate represented only one of the mating type genes. Overall data sets presented 1:1 ratio of mating types for both species, indicating a possible sexual reproduction. No polymorphisms were detected in partial sequences of mating type genes. The isolates werein vitrotested for their pathogenicity using apple fruits as a host and aggressiveness were evaluated at different time points using lesion sizes.Monilinia laxawas found more aggressive thanM. fructicola. Moreover,M. fructicolaisolates displayed larger colony growth compared toM. laxaisolates. However, no significant correlation was detected between the colony growth rates and virulence. This study is the first to demonstrate brown rot pathogens of peach fruit in Turkey and identifies different aspects of the pathogens, which would be useful in containment of pathogen spread and comparison with other populations of these pathogens worldwide, and suggests a new set of mating type markers for these species.