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    Biosynthesis of gold nanoparticles using marine bacteria and Box-Behnken design optimization
    (Taylor & Francis Inc, 2019) Camas, Mustafa; Celik, Fatih; Camas, Anil Sazak; Ozalp, Hasan Baris
    Gold nanoparticles are exciting materials because of their potential applications in optics, electronics, biomedical, and pharmaceutical fields. In recent years, environmentally friendly, low-cost biosynthesis methods with bio-applicable features have continued to be developed for the synthesis of gold nanoparticles. In the present study, an actinobacterial strain was isolated from the Petrosia ficiformis (Poiret 1798) sponge, which was collected from a marine environment, and the gold nanoparticle synthesis was performed for the first time from the bacteria type belonging to the Citricoccus genus. The synthesis conditions were optimized using the Box-Behnken experimental design, with a statistical method that included three independent variables (temperature, time, and mixture ratio) to affect the synthesis at three levels (+1, 0, and -1). Accordingly, the conditions proposed for the biosynthesis of gold nanoparticles at the maximum optical density values that are specific for the Citricoccus sp. K1D109 strain were estimated as 35 degrees C temperature, 24 h, and 1/5 mixture ratio (cell-free extract/HAuCl4 center dot 3H(2)O). When recommended conditions were applied, it was determined that the maximum absorbance of the synthesized gold nanoparticles is 1.258 at 545 nm, and their sizes are in the range of 25-65 nm, according to transmission electron microscopy (TEM) data.
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    Fisherman's Shelters around Canakkale Strait, Turkey
    (Mediterranean Coastal Foundation-Medcoast Foundation, 2007) Alparsan, Mustafa; Ozalp, Hasan Baris
    Northwestern Turkey is divided by a complex waterway that connects the Black Sea to the Sea of Marmara and the Aegean Sea. The Turkish Straits, comprising the Canakkale Strait, the Strait of Istanbul and the Sea of Marmara and are unique in many respects. There are 24 fisherman's shelters around the Canakkale Strait and have very different structures. The first duty of the fisherman's shelter is to serve all manner of fishing vessels. The fisherman's shelters, both for fishery and for the acitivites of the ministry to be fulfilled, have a vital status and importance. Such places not only serve as a sheltering, safekeeping and service area for fisherman's vessels, but also as the first point to which the fishermen's catch has been put. Because of these characteristics, the inspectorships done by the Ministry of Agriculture and Rural Affairs are mostly carried out in these areas. Although our fisherman's shelters are sufficient in number, some of them need to be developed their infrastructures and superstructures.
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    Population dynamics of a temperate coral along a depth gradient in the Dardanelles
    (Wiley, 2020) Caroselli, Erik; Ozalp, Hasan Baris; Lavia, Maila; De Witt, Francesca; Raimondi, Francesco; Goffredo, Stefano
    Notwithstanding the importance of analyzing the variations of population dynamic traits along environmental gradients for assessing coral resilience to global climate change, temperate areas are more understudied than tropical regions. In the Mediterranean Sea, some studies are available for its western basin, while there is a lack of information for its eastern basin. This study starts to fill this gap, by determining the demographic characteristics of the solitary zooxanthellate scleractinianBalanophyllia europaeain the Dardanelles (Turkey) along a 1-21 m depth transect. The population dynamics ofB. europaeafrom this site in the eastern Mediterranean Sea was compared with the populations in the NW Mediterranean Sea. In particular, the sites of Calafuria and Palinuro (Tyrrhenian Sea, Italy) were chosen for the comparison since their temperature and latitude, respectively, were similar to that of the Dardanelles population. The population density in the Dardanelles increased exponentially with depth, possibly due to lower current and wave action, and higher salinity at depth. Age structure in the Dardanelles was relatively overrepresented in younger age classes with increasing depth. Nevertheless, this difference in age structure was not strong enough to cause trends with depth for all derived demographic parameters. In comparison with Italian populations, age structure presented a higher frequency of young individuals and were more stable in the Dardanelles, likely due to the less intense wave action. Hosting different clades of symbiotic algae may also contribute to these differences, but molecular studies are necessary to verify this hypothesis.
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    The first record of Cladocora caespitosa (Linnaeus, 1767) (Anthozoa, Scleractinia) from the Marmara Sea
    (Tubitak Scientific & Technological Research Council Turkey, 2011) Ozalp, Hasan Baris; Alparslan, Mustafa
    The Scleractinian Cladocora caespitosa is reported for the first time from the Marmara Sea, Turkey. Five colonies of the elliptical Cladocora caespitosa were discovered at 7 m by scuba divers around Posidonia oceanica beds colonising a substrate of sand and rock. The colonies' characteristics were recorded with underwater apparatus with a close-up device and with a video system. The present paper provides the new updated area of the species on the Turkish coast.
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    Uranium (VI) Biosorption on Marine Sponge, Sarcotragus foetidus (Schmidt, 1862) and Its Statistical Investigation Using Central Composite Design
    (Central Fisheries Research Inst, 2016) Celik, Fatih; Camas, Mustafa; Camas, Anil Sazak; Ozalp, Hasan Baris
    The biosorption of heavy metals, radionuclides, uranium and thorium for the recovery or removal from aqueous systems using various micro-organisms and biological substances has been investigated by several groups of workers. In this paper, the uptake of uranium (VI) from aqueous systems by a marine sponge, Sarcotragus foetidus (Schmidt, 1862) has been investigated. S. foetidus samples were collected from Babakale/Ayvacik/Canakkale in Turkey. Uranium (VI) uptake experiments were carried out by the batch technique. The affecting parameters were analyzed using Central Composite Design (CCD) as the experimental method. Four independent variables such-pH, temperature, contact time, and initial uranium concentration were selected for this study. The influences of initial pH, contact time, and initial uranium concentration on the biosorption process were considered statistically significant as main effects. On the other hand, interaction effects of pH and contact time, as well as pH and initial uranium concentration, were considered as significant parameters on the biosorption process. The optimum condition of biosorption process was found to be at initial pH: 4, temperature: 35 degrees C, initial uranium concentration: 20 mg/L, and contact time: 31 minutes. At these conditions, the biosorption yield of uranium (VI) ions was obtained to be 93.29 +/- 2%.