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    Evalution of antibacterial activities of some antiseptics and disinfectants
    (Refik Saydam National Public Health Agency (RSNPHA), 2017) Avci, Derya; Otkun, Müserref
    Objective: In our study, we aimed to investigate the effects and duration of effect of 4 antiseptics and disinfectants frequently used in hospitals on 12 different resistant species of bacteria that cause nosocomial infections which were isolated from inpatients in our hospital. Methods: Three strains each of Acinetobacter baumannii, Pseudomonas aeruginosa, methicillinresistant Staphylococcus aureus and vancomycin-resistant Enterococcus were chosen for the study. Three American Type Culture Collection standard bacteria strains (ATCC) including P.aeruginosa ATCC 27853, Escherichia coli ATCC 25922, and Staphylococcus aureus ATCC 6538 were used as the control group. Antiseptics and disinfectants were evaluated at different concentrations and included povidone iodine (10%), sodium hypochloride (5%), ethyl alcohol and gluteraldehyde (2%) which are routinely used for disinfection in our hospital. Povidone iodine (10%) and gluteraldehyde (2%) were evaluated in 3 forms; undiluted according to the manufacturer's instructions and also in 1/2 and 1/4 diluted forms. Sodium hypochlorite (5%) was examined in undiluted form, and at 1/10 and 1/100 dilution. Ethyl alcohol was prepared at the concentrations of 95%, 70%, and 50% before use. The selected bacteria were contacted with the materials for 1, 2, 3, 10, 30 minutes by using the qualitative suspension test method. Before seeding, a neutralization solution was added to stop the effects of the material. After incubation, the bacteria were assessed for the presence or absence of proliferation. Results: In this study, povidone iodine (10%), which inhibited the growth of all bacteria, was detected the most effective antiseptic at all dilutions after 1 minute of contact. Gluteraldehyde (2%), which inhibited the growth of all bacteria, was the most powerful disinfectant at both 1/2 dilution and in undiluted form after 1 minute of contact. It was determined that surface contact of at least 2 minutes duration is appropriate for 70% ethyl alcohol. When sodium hypochlorite was used in 1/10 dilution, it was suitable to inhibit all bacteria by 1 minute contact, while at least 5 minutes to kill all the bacteria in 1/100 dilution. Conclusion: For protection of patients and healthcare providers, disinfection policies should be created especially for all hospitals. Detecting effective antiseptic and disinfectants for each patients own microorganisms will be both cost and safe for healthcare environment.