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    Heterorhabditis floridensis n. sp. (Rhabditida: Heterorhabditidae) from Florida
    (Magnolia Press, 2006) Nguyen, Khuong B.; Gozel, Ugur; Köppenhöfer, Heather S.; Adams, Byron J.
    In a survey of entomopathogenic nematodes associated with plants and trees in areas adjacent to production citrus groves in Florida, a new species of nematode in the genus Heterorhabditis was found based of morphological and molecular studies. The new nematode is described as Heterorhabditis floridensis n. sp. H. floridensis n. sp. is characterized by males, females, and infective juveniles. For males, the number of papillae in the terminal group of bursa is variable, either with 2 pairs of papillae (40%), with 3 papillae on one side and 2 papillae on the other side (30%), with one pair of papillae (20%), or with three pairs of papillae (10%). SW and GS values are 179 and 50, respectively. Females have a typical vulva pattern, which is different from that of closely related nematode species H. bacteriophora, H. mexicana, and H. indica. For infective juveniles, EP=109 (101-122) ?m, ES=135 (123-142) ?m, tail length=103 (91-113) ?m, and a=27.6 (25-32) are different from those of the above-mentioned three related nematodes. Phylogenetic analysis based on ITS regions show that the new species forms a clade with H. mexicana, H. baujardi and H. indica and differs from these species by several nucleotide autapomorphies. Copyright © 2006 Magnolia Press.
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    Steinernema khoisanae n. sp (Rhabditida:Steinernematidae), a new entomopathogenic nematode from South Africa
    (Brill, 2006) Nguyen, Khuong B.; Malan, Antoinette P.; Gozel, Ugur
    A new species of entomopathogenic nematode, Steinernema khoisanae n. sp. is described from South Africa. The new species is characterised by morphometrics of the infective juvenile with body length 1076 mu m, narrow body diameter of 33 mu m, excretory pore 94 mu m from anterior end, tail 85 mu m long, a = 33, D% = 68, H% = 57, and E% = 111. The lateral field pattern of the new species is 2, 7, 8, 6, 4 and 2. The male of the first generation can be recognised by the spicule and the gubernaculum shape, excretory pore located posteriorly near the end of the pharynx, D% = 88, and SW% = 199. The first generation female can be recognised by the non-protruding vulva and tail bearing a prominent mucron. Steinernema khoisanae n. sp. is characterised genetically by sequences of the internal transcribed spacers and D2/D3 regions of 28S ribosomal DNA, by composition of their sequences and by numerous unique, derived, nucleotide character states. Phylogenetic trees show that S. khoisanae n. sp. and other members of the S. glaseri-group form a monophyletic assemblage.
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    Steinernema texanum n. sp (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Texas, USA
    (Brill, 2007) Nguyen, Khuong B.; Stuart, Robin J.; Andalo, Vanessa; Gozel, Ugur; Rogers, Michael E.
    Steinernema texanum n. sp. is characterised by morphometrics of the infective juvenile with body length = 756 mu m, distance from anterior end to the excretory pore = 59 mu m, tail = 73 mu m, ratio a = 25, H% = 59 and E% = 81. The lateral field pattern of the new species is 2, 7, 2, and is typical for the species. The male of the first generation can be recognised by the spicule and the gubernaculum lengths and shapes, position of the excretory pore, D% = 67 and GS% = 75. The female can be recognised by the vulva with very low epiptygma and two wart-like structures anterior to the tail tip that are always present on the ventral side. Steinernema texanum n. sp. is characterised genetically by the sequence of the ITS region (sequence length = 956 bp, the length of ITS I = 263 bp, ITS2 = 286 bp, composition of its sequence and by 18 autapomorphies) and by sequence of D2D3 regions (sequence length = 860 bp, its composition and 15 autapomorphies). Steinernema texanum n. sp. is closely related to species in the feltiae-group, which include S. akhursti, S. feltiae, S. hebeiense, S. jollieti, S. kraussei, S. kushidai, S. litorale, S. monticolum, S. oregonense, S. sangi, S. silvaticum and S. weiseri. Isolates of the new species were obtained using the Galleria-baiting technique from soil samples taken near Kingsville, Texas, USA.