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Öğe 3D HYDROGELS FROM DETERGENT-FREE DECELLULARIZED SPINAL CORD MENINGES REINFORCED WITH HYDROPHILIC SILK FIBROIN FOR REGENERATIVE MEDICINE APPLICATIONS(Mary Ann Liebert, Inc, 2024) Kurt, Tuğçe; Arslan, Yavuz Emre[No abstract available]Öğe 3D-Printable, Self-Stiffening (4D) and Shape Morphing Hydrogel through Single-Step Orthogonal Crosslinking of Phenolic Biopolymers for Dynamic Tissue Engineering(Wiley, 2025) Güngör, Nuriye Nazet; Kurt, Tuğçe; Sarı, Buse; Işık, Melis; Okesola, Babatunde O.; Arslan, Yavuz Emre; Derkuş, BurakParticularly for dynamic, shape-changing, or fibrillar tissues such as muscles and blood vessels, the development of innovative biomaterials is crucial for advancing tissue engineering and regenerative medicine. This study introduces a novel multicomponent hydrogel created from silk fibroin (SF), tyramine-modified hyaluronic acid (HA_Tyr), and tyramine-modified gelatin (G_Tyr). Using an enzymatic orthogonal covalent bonding between phenolic groups, i.e., tyrosine and tyramine moieties of SF, HA_Tyr, and G_Tyr, a dynamically stiffening SF/HA_Tyr/G_Tyr (SHG) multicomponent hydrogel is achieved with enhanced mechanical properties. Utilizing an extrusion-based 3D printing approach, the precise fabrication of constructs with tailored geometries and functionalities is demonstrated. The emerging 3D-printed hydrogels undergo morphologic changes (4D) under 37 °C/phosphate buffer saline (PBS) conditions. The observed morphological change results from the conformational change and folding of SF leading to fibrillation. These multicomponent hydrogels also show significant promise in creating bio-instructive materials that meet the mechanical and functional requirements necessary for in situ tissue engineering. The study highlights the potential of these self-stiffening biomaterials to recover dynamic and fibrillar tissues, supported by both in vitro and pre-clinical chorioallantoic membrane (CAM) model evaluations that underscore their biocompatibility and pro-angiogenic properties.Öğe Clinical tissue engineering approach and biotechnological advances to improve athlete healthcare(Turkish Sports Medicine Association, 2023) Demiray, Elif Beyza; Kurt, Tuğçe; Duman, Zeynep Yağmur; Özdemir, Büşra Nur; Erkovan, Burak; Yiğit, Gaye Su; Arslan, Yavuz EmreSports activities have continued for centuries and have become essential to daily life. Professional athletes participating in various sports competitions have many advantages, such as a promising career and high income. On the other hand, being a professional athlete also has some disadvantages. The most dramatic one is the risk of injury. Even though injuries are a part of sports, they have become a significant problem today due to the long recovery period which in turn overshadows sports competitions. In addition, the performance loss is an extra handicap for the athletes compared to the pre-injury levels. In this case, biomedical and biotechnological sciences are a glimmer of hope for shortening the treatment process and minimizing performance loss in returning to professional sports life. Combinations of scaffolds, biological factors, and cells are utilized based on mentioned approaches to treat such injuries, which are frequently seen nowadays and have become the nightmare of professional athletes. This review discusses various regenerative medicine and biotechnology-based therapeutic methods used in the treatment of spinal cord, cartilage, tendon, and musculoskeletal injuries in athletes. Additionally, wearable technologies, which are used to evaluate physiological signals, monitor health, prevent possible injuries, and create personalized training programs are mentioned, as well.Öğe Decellularization of bovine spinal cord meninges via supercritical CO2 and evaluating the extracellular matrix performance for neural tissue engineering applications(Mary Ann Liebert, Inc, 2024) Kurt, Tuğçe; Özüdoğru, Eren; Cengiz, Uğur; Derkuş, B.; Arslan, Yavuz Emre[No abstract available]Öğe Decellularized tumor matrices as biomimetic cancer niche: a new perspective on cancer research and therapy(Iop Publishing Ltd, 2025) Özüdoğru, Eren; Kurt, Tuğçe; Arslan, Yavuz EmreCancer is among the major causes of mortality, responsible for approximately 15% of all deaths worldwide. Despite remarkable progress in modern medicine, it remains a significant global health challenge. Nevertheless, conventional therapies such as chemotherapy and radiotherapy target healthy and malignant tissues, leading to adverse side effects, including hair loss, fatigue, and nausea, which significantly reduce patients' quality of life. Even more critically, the therapeutic response varies from patient to patient, which reduces the effectiveness of treatment. Therefore, cancer tissue engineering has evolved as a novel interdisciplinary field, aiming to develop structures that mimic the tumor microenvironment to elucidate cancer development mechanisms and devise effective treatment methods. However, producing a fully synthetic biosimilar matrix by assembling all individual ECM components remains unfeasible due to the heterogeneity and complex structure of tumor tissues, as well as the necessity of highly advanced micro- and nanoengineering techniques. Consequently, decellularization techniques have recently been applied to cancer tissues to produce biomimetic tumor models. In this review, we provided a comprehensive overview of the extracellular matrix (ECM) architecture and its role in tumor progression. We also discussed the structural differences between normal and malignant tissues. We briefly reviewed decellularization techniques and analytical approaches for ECM characterization. Emphasizing the cutting-edge research, we categorized developments into three groups: decellularized tumor-derived ECM (dT-ECM), hydrogels, and bioinks. Subsequently, we critically assessed the benefits, limitations, and potential future developments of dT-ECM-based strategies. Finally, we envision that tumor tissue engineering will provide preventive treatment approaches by developing patient-specific predictive and personalized cancer models through integrating advanced biomaterials with artificial intelligence and machine learning.Öğe Rejenere ipek fibroini/hücresizleştirilmiş omurilik meninks kaynaklı doku mühendisliği yapı iskelelerinin üretimi ve karakterizasyonu(Çanakkale Onsekiz Mart Üniversitesi, 2025) Kurt, Tuğçe; Arslan, Yavuz EmreSinir sisteminde meydana gelen yapısal hasarların kalıcı fonksiyon kayıplarına yol açması, bu alanda etkili biyomalzemelerin geliştirilmesini hayati hale getirmiştir. Özellikle omurilik yaralanmalarında rejeneratif kapasitenin sınırlı olması, doku mühendisliği temelli çözümlerin önemini artırmaktadır. Bu çalışmada, hedef dokuya biyokimyasal ve yapısal açıdan benzerlik gösteren özgün bir hidrojel sistemi geliştirilmiştir. Deterjan içermeyen, ultrasonik destekli bir yöntemle hücresizleştirilen spinal kord meninks dokusundan elde edilen hücre dışı matriks (HDM), çözünürlüğü iyileştirilmiş, liyofilize edilerek stabil formda elde edilen suda çözünebilir ipek fibroin (İF) ile birleştirilmiştir. Ortaya çıkan kompozit sistem, HRP/H₂O₂ aracılığıyla enzimatik olarak çapraz bağlanarak üç boyutlu hidrojel yapısına dönüştürülmüştür. Karakterizasyon sürecinde, çift sarmal DNA, hidroksiprolin ve glikozaminoglikan içerikleri kantitatif olarak analiz edilmiş; agaroz jel ve SDS-PAGE teknikleriyle hücresel kalıntılar değerlendirilmiştir. SEM görüntüleme, mikro mekanik ve reolojik testlerle yapısal özellikler; ATR-FTIR, XRD, TGA ve BET analizleriyle kimyasal ve fiziksel nitelikler ortaya konmuştur. Şişme, temas açısı ve in vitro bozunurluk testleriyle fonksiyonel parametreler incelenmiştir. Hücre kültürü deneylerinde nöroblastoma hücre hattı kullanılarak canlılık, proliferasyon ve miyojenik gen ekspresyonu (RT-qPCR) analiz edilmiştir. CAM modeliyle de anjiyojenik potansiyel doğrulanmıştır. Elde edilen sonuçlar, geliştirilen dSCM-İF hidrojel sisteminin sinir doku mühendisliği için biyofonksiyonel bir platform sunduğunu göstermektedir.
