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    Öğe
    Comparison of the growth characteristics in two different Spirulina platensis strains
    (Ege Univ, 2006) Kilic, Cenker; Goksan, Tolga; Ak, Ilknur; Gokpinar, Sevket
    The cultivation of Spirulina platensis, which is often used in microalgal biotechnology, has successfully been carried out in our country as well. In this study, the growth characteristics of two Spirulina strains in straight and spiral forms were investigated. In addition, spectrophotometric measurements, which make the measurements of chlorophyll and dry weight more practical, were studied. It was found that all the parameters except the cell count were similar during the experiment. While the spiral/straight form ratio was 2.43 at the beginning of the experiment, it increased up to 5.53 at the end. Spectrophotometric measurements are of importance in terms of practicality in the monitoring of the growth in the cultures. In this respect, a strong correlation was determined between the absorbance values at 680 nm and the amounts of dry weight and chlorophyll (p<0.05). As a result, though there was not a significant difference between the growth parameters of the both forms, the use of straight one would be better due to the problems encountered during harvesting.
  • [ X ]
    Öğe
    Growth Characteristics of the Alga Haematococcus pluvialis Flotow as Affected by Nitrogen Source, Vitamin, Light and Aeration
    (Central Fisheries Research Inst, 2011) Goksan, Tolga; Ak, Ilknur; Kilic, Cenker
    In the present study, the effects of various inorganic nitrogen sources, light intensities, aeration rates and especially vitamins were investigated in vegetative Haematococcus pluvialis Flotow cultures. The best growth among the nitrogen sources was achieved in NaNO(3) (1.0 g/L) and KNO(3) (0.5 g/L) with cell densities of 25.3 and 26.3x10(4) cells/ml respectively. In vitamin trials, the highest cell numbers were found in 0.1(0.3 mu M), 1.0 (4 mu M) and 0.1 ing/L (0.75 mu M) concentrations for thiamin, biotin and B12 respectively. It was also seen that the use of thiamin alone was sufficient instead of using the vitamin mix. Cell growth was significantly higher in 75 and 150 mu mol photon m(-2) s(-1) illuminations compared to 20 and 40 mu mol photon m(-2) s(-1). As for aeration, optimum airflow rate was 1.0 lpm, while supra-optimum levels ceased the growth, cell diameter and pigment content increased. So, it may play a role as a secondary stress factor on H. pluvialis cells in reddening stage.