Yazar "Keskin, Emre" seçeneğine göre listele

Listeleniyor 1 - 3 / 3
  • Küçük Resim
    Öğe
    Can DNA Barcode Study be Done from a Museum Specimen Fixed in a Formaldehyde Solution? A Case of Emys orbicularis (Linnaeus, 1758)
    (Çanakkale Onsekiz Mart Üniversitesi, 2024) Günay, Ulvi Kerem; Yakın, Batuhan Yaman; Pipilos, Atakan; Keskin, Emre; Tok, Cemal Varol
    DNA barcoding, a molecular taxonomy technique, has been increasingly used by herptile taxonomists in recent years. In DNA barcoding studies with museum specimens, there are difficulties in achieving success in specimens that have been exposed to formaldehyde, which is usually used as a fixative, for a long time and intensively. Here we studied the effect of formaldehyde on the application of the DNA barcode method in Emys orbicularis specimens stored in 4% formaldehyde and 70% ethanol solution since 2008 and 2014. Sanger sequence analysis of tissues taken from samples stored in both ethanol and formaldehyde solution successfully yielded sequences of 623 bp. In conclusion, the use of ethanol solutions should be preferred for mid or long-term sample storage, especially in the context of molecular studies. In cases where the use of formaldehyde is unavoidable, it may be advisable to use extremely low concentrations to increase success in molecular research.
  • [ X ]
    Öğe
    Genetic structuring of Anguis fragilis (L., 1758) inhabiting in the north of 40° north latitude in Turkey
    (Taylor & Francis Ltd, 2013) Keskin, Emre; Hayretdag, Sibel; Cicek, Kerim; Ayaz, Dincer; Tok, Cemal Varol
    The taxonomic situation of Anguis fragilis species is still unclear in Turkey. In order to clarify this situation, we used the DNA sequences of 16S rRNA and cytochrome b genes to analyze the phylogenetic relationship among A. fragilis populations. A total of 13 haplotypes in 16S rRNA dataset and 20 haplotypes in cytochrome b dataset were detected. Kimura 2-parameter genetic distance was found to be 0.012 for 16S rRNA and 0.026 for the cytochrome b dataset. Neighbor joining (NJ) trees were constructed to analyze phylogenetic relationship among specimens and were supported with median joining networks. Results indicate a clear genetic structuring in A. fragilis populations sampled from north of 408 north latitude of Turkey. Both mitochondrial gene sequences successfully detected the intraspecific variation among specimens of different populations. Genetic structuring, correlated with geographic distance, was found to be significant at the specimens sampled from edge populations of peripherally isolated climatic conditions.
  • [ X ]
    Öğe
    Genetic structuring of Pseudopus apodus (Pallas, 1775) (Sauria: Anguidae) in north Anatolia, Turkey
    (Pergamon-Elsevier Science Ltd, 2013) Keskin, Emre; Tok, Cemal Varol; Hayretdag, Sibel; Cicek, Kerim; Ayaz, Dincer
    In this study, mitochondrial 16S rRNA and cytochrome b sequences were used to infer the genetic structure of Pseudopus apodus (Pallas, 1775) populations sampled from 40 North latitude of Turkey. Mean percent G-C contents of 47.13 and 46.66 were determined for 16S rRNA and cytochrome b datasets, respectively. Two haplotypes were found using 16S rRNA dataset, with 6 variable loci and a haplotype diversity of 0.1053, while Cytochrome b dataset consist of 10 haplotypes, with 18 variable loci and a haplotype diversity of 0.1943. Also mean genetic distance was found to be eight times higher in the cytochrome b dataset. Kimura 2-parameter genetic distance matrix was evaluated together with neighbor joining tree and median joining network in order to reveal possible divergence points in both datasets. Results indicated signs of 3 regional populations, including potential cryptic species from Digor or subspecies from Samsun and Sinop. (C) 2013 Elsevier Ltd. All rights reserved.